Control of pepsinogen synthesis and secretion in primary monolayer cultures of canine gastric chief cells

1988 ◽  
Vol 33 (12) ◽  
pp. 1583-1591 ◽  
Author(s):  
J. Defize ◽  
R. H. Hunt
Keyword(s):  
Chief Cells ◽  
Monolayer Cultures ◽  
Primary Monolayer ◽  
Primary Monolayer Cultures

Adaptability of marine teleost renal inorganic sulfate excretion: evidence for glucocorticoid involvement

1989 ◽  
Vol 257 (3) ◽  
pp. R511-R516 ◽  
Author(s):  
J. L. Renfro
Keyword(s):  
Culture Medium ◽  
Brush Border Membrane ◽  
Renal Tubule ◽  
Membrane Vesicles ◽  
Renal Tubules ◽  
Pseudopleuronectes Americanus ◽  
Monolayer Cultures ◽  
Primary Monolayer ◽  
Primary Monolayer Cultures ◽  
Inorganic Sulfate

The marine winter flounder, Pseudopleuronectes americanus, ingests seawater (SW) and excretes most of the resultant divalent ion load by renal tubule secretion. The site of secretion is generally thought to be the proximal tubule. Although sulfate clearance ratios (C ratio-sulfate clearance/polyethylene glycol clearance) have been reported as high as 12 in SW-acclimated animals, the present study shows that net secretion ceases after acclimation to 10% SW (SO4 free). Intravenous infusion of sulfate into the latter induced net secretion within 5 h (C ratio increased from 0.83 +/- 0.23 to 2.12 +/- 0.33). Increased sulfate secretion coincided with an increase in the magnitude of HCO3-SO4 exchange in brush-border membrane vesicles (BBMV) isolated from flounder renal tubules. Treatment of 10% SW-acclimated flounder with 60 micrograms dexamethasone/100 g body wt also caused an increase in the HCO3-SO4 exchange in BBMV to a level comparable to that of BBMV from SW fish. The glucocorticoid effect was further tested in flounder renal tubule primary monolayer cultures. Measurement of unidirectional sulfate fluxes (J) showed that in the presence of cortisol net secretion (serosal-to-mucosal flux) dominated (Js----m = 111 +/- 6.5, Jm----s = 9 +/- 4.3, Jnet = 102 +/- 2.2 nmol.h-1.cm-2). Removal of cortisol from the culture medium significantly reduced net sulfate secretion to one-third of control (Js----m = 39 +/- 13.9, Jm----s = 6 +/- 1.0, Jnet = 33 +/- 14.1 nmol.h-1.cm-2).

scholarly journals RELATIONSHIP OF DONOR AGE TO IN VITRO PRODUCTION OF FOOT-AND-MOUTH DISEASE VIRUS BY MOUSE KIDNEY CELLS

1965 ◽  
Vol 121 (1) ◽  
pp. 69-83 ◽  
Author(s):  
Charles H. Campbell
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Virus Multiplication ◽  
Mouth Disease ◽  
Kidney Cells ◽  
Monolayer Cultures ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Primary Monolayer ◽  
Primary Monolayer Cultures

Multiplication of foot-and-mouth disease virus (FMDV) was compared in kidney cells from 7- to 35-day-old mice representing various degrees of age resistance to this virus. Three types of cell preparations were used: primary monolayer cultures, suspensions of dispersed cells, and suspensions of minced tissue. Virus multiplication in the two types of cell suspensions was related to the age of the donors both in regard to time when multiplication first became evident and to the amount of virus produced. While adsorption rates were similar in the cells from all age groups, virus multiplication began earlier in cells from younger mice and more virus was produced by these cells than by cells from older animals. There was no significant difference in the virus growth rates in the primary monolayer cultures of cells. The results indicate that kidney cells from mice 7 to 35 days old vary in their ability to produce virus in relation to the degree of susceptibility of the cell donors. After propagation of the cells in primary monolayer cultures, however, this difference no longer exists probably because of cell selection under the cultural conditions.

Sign in / Sign up

Export Citation Format

Share Document