Distinction between vascular smooth muscle cells and myoepithelial cells in primary monolayer cultures of human breast tissue

1989 ◽  
Vol 25 (3) ◽  
pp. 259-266 ◽  
Author(s):  
O. W. Petersen ◽  
B. Deurs
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Breast Tissue ◽  
Myoepithelial Cells ◽  
Human Breast ◽  
Human Breast Tissue ◽  
Monolayer Cultures ◽  
Primary Monolayer ◽  
Primary Monolayer Cultures

scholarly journals A fibroblast-associated antigen: characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells.

1992 ◽  
Vol 40 (4) ◽  
pp. 475-486 ◽  
Author(s):  
L Rønnov-Jessen ◽  
J E Celis ◽  
B Van Deurs ◽  
O W Petersen
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Stromal Cells ◽  
Breast Tissue ◽  
Muscle Cells ◽  
Human Breast ◽  
Human Breast Tissue ◽  
Coated Pits

Fibroblasts with smooth muscle differentiation are frequently derived from human breast tissue. Immunofluorescence cytochemistry of a fibroblast-associated antigen recognized by a monoclonal antibody (MAb), 1B10, was analyzed with a view to discriminating smooth muscle differentiated fibroblasts from vascular smooth muscle cells. The antigen was detected on the cell surface and in cathepsin D-positive and acridine orange-accumulating vesicular compartments of fibroblasts. Ultrastructurally, the antigen was revealed in coated pits and in endosomal and lysosomal structures. 1B10 recognized three major brands migrating at apparent Mr of 38,000, 45,000, and 80,000, in addition to many minor bands between Mr 45,000 and 97,000, including Mr 52,000. The Mr 45,000 and 38,000 were associated with the cell membrane and Mr 52,000 as well as Mr 38,000 were associated with the lysosomes. The 1B10 immunoreactivity was specific to fibroblasts and smooth muscle differentiated fibroblasts within the context of vascular smooth muscle cells.

scholarly journals Expression of smooth muscle-specific proteins in myoepithelium and stromal myofibroblasts of normal and malignant human breast tissue.

1993 ◽  
Vol 90 (3) ◽  
pp. 999-1003 ◽  
Author(s):  
D. Lazard ◽  
X. Sastre ◽  
M. G. Frid ◽  
M. A. Glukhova ◽  
J. P. Thiery ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Breast Tissue ◽  
Human Breast ◽  
Human Breast Tissue ◽  
Specific Proteins
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