Specific chromosome loss associated with the expression of tumorigenicity in human cell hybrids

1981 ◽  
Vol 7 (6) ◽  
pp. 699-712 ◽  
Author(s):  
Eric J. Stanbridge ◽  
Robert R. Flandermeyer ◽  
David W. Daniels ◽  
Walter A. Nelson-Rees
Keyword(s):  
Human Cell ◽  
Chromosome Loss ◽  
Specific Chromosome ◽  
Cell Hybrids

Chromosome segregation from cell hybrids. VII. Reverse segregation from karyoplast hybrids suggests control by cytoplasmic factors

Genome ◽  
1992 ◽  
Vol 35 (3) ◽  
pp. 537-540 ◽  
Author(s):  
Jennifer A. Marshall Graves ◽  
Iole Barbieri
Keyword(s):  
Chromosome Segregation ◽  
Human Cell ◽  
Chinese Hamster ◽  
Segregation Pattern ◽  
Chromosome Loss ◽  
Human Chromosomes ◽  
Cell Hybrids ◽  
Cytoplasmic Factors

Using human and Chinese hamster established lines as cell parents, we constructed hamster–human cell hybrids and human cell – hamster karyoplast hybrids. The cell hybrids retained one or two sets of hamster chromosomes and lost most of the human chromosomes. The karyoplast hybrids, however, retained a full set of human chromosomes and lost most of the Chinese hamster chromosomes. This reverse segregation pattern implies that cytoplasmic factors are major determinants of the direction of chromosome segregation.Key words: cell hybrids, chromosome loss, cytoplasmic factors, reverse segregation.

Segregation of recessive phenotypes in somatic cell hybrids: role of mitotic recombination, gene inactivation, and chromosome nondisjunction

1981 ◽  
Vol 1 (4) ◽  
pp. 336-346
Author(s):  
C E Campbell ◽  
R G Worton
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Mitotic Recombination ◽  
Chinese Hamster Cell ◽  
Gene Inactivation ◽  
Chromosome Loss ◽  
Resistance Locus ◽  
Chromosome 2 ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Somatic cell hybrids heterozygous at the emetine resistance locus (emtr/emt+) or the chromate resistance locus (chrr/chr+) are known to segregate the recessive drug resistance phenotype at high frequency. We have examined mechanisms of segregation in Chinese hamster cell hybrids heterozygous at these two loci, both of which map to the long arm of Chinese hamster chromosome 2. To follow the fate of chromosomal arms through the segregation process, our hybrids were also heterozygous at the mtx (methotrexate resistance) locus on the short arm of chromosome 2 and carried cytogenetically marked chromosomes with either a short-arm deletion (2p-) or a long-arm addition (2q+). Karyotype and phenotype analysis of emetine- or chromate-resistant segregants from such hybrids allowed us to distinguish four potential segregation mechanisms: (i) loss of the emt+- or chr+-bearing chromosome; (ii) mitotic recombination between the centromere and the emt or chr loci, giving rise to homozygous resistant segregants; (iii) inactivation of the emt+ or chr+ alleles; and (iv) loss of the emt+- or chr+-bearing chromosome with duplication of the homologous chromosome carrying the emtr or chrr allele. Of 48 independent segregants examined, only 9 (20%) arose by simple chromosome loss. Two segregants (4%) were consistent with a gene inactivation mechanism, but because of their rarity, other mechanisms such as mutation or submicroscopic deletion could not be excluded. Twenty-one segregants (44%) arose by either mitotic recombination or chromosome loss and duplication; the two mechanisms were not distinguishable in that experiment. Finally, in hybrids allowing these two mechanisms to be distinguished, 15 segregants (31%) arose by chromosome loss and duplication, and none arose by mitotic recombination.

Human cell hybrids: analysis of transformation and tumorigenicity

Science ◽  
1982 ◽  
Vol 215 (4530) ◽  
pp. 252-259 ◽  
Author(s):  
E. Stanbridge ◽  
C. Der ◽  
C. Doersen ◽  
R. Nishimi ◽  
D. Peehl ◽  
...  
Keyword(s):  
Human Cell ◽  
Cell Hybrids

scholarly journals Human Chromosomes 9, 12, and 15 Contain the Nucleation Sites of Stress-Induced Nuclear Bodies

2002 ◽  
Vol 13 (6) ◽  
pp. 2069-2079 ◽  
Author(s):  
Marco Denegri ◽  
Daniela Moralli ◽  
Mariano Rocchi ◽  
Marco Biggiogera ◽  
Elena Raimondi ◽  
...  
Keyword(s):  
Heat Shock ◽  
Human Cell ◽  
Hela Cells ◽  
Heat Shock Factor ◽  
Nuclear Bodies ◽  
Chromosome 9 ◽  
Heat Shock Factor 1 ◽  
Human Chromosomes ◽  
Cell Hybrids ◽  
Nucleation Sites

We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Serine-Arginine splicing factors. In this article, we show that stress-induced SNBs are detectable in human but not in hamster cells. By means of hamster>human cell hybrids, we have identified three human chromosomes (9, 12, and 15) that are individually able to direct the formation of stress bodies in hamster cells. Similarly to stress-induced SNB, these bodies are sites of accumulation of hnRNP A1-interacting protein and heat shock factor 1, are usually associated to nucleoli, and consist of clusters of perichromatin granules. We show that the p13-q13 region of human chromosome 9 is sufficient to direct the formation of stress bodies in hamster>human cell hybrids. Fluorescence in situ hybridization experiments demonstrate that the pericentromeric heterochromatic q12 band of chromosome 9 and the centromeric regions of chromosomes 12 and 15 colocalize with stress-induced SNBs in human cells. Our data indicate that human chromosomes 9, 12, and 15 contain the nucleation sites of stress bodies in heat-shocked HeLa cells.

Identification of a deletion in the mismatch repair gene, MSH2, using mouse-human cell hybrids monosomal for chromosome 2

2003 ◽  
Vol 63 (3) ◽  
pp. 215-218 ◽  
Author(s):  
RE Pyatt ◽  
H Nakagawa ◽  
H Hampel ◽  
M Sedra ◽  
MB Fuchik ◽  
...  
Keyword(s):  
Mismatch Repair ◽  
Human Cell ◽  
Mismatch Repair Gene ◽  
Chromosome 2 ◽  
Repair Gene ◽  
Cell Hybrids

Asynchronous DNA replication and asymmetrical chromosome loss in Chinese hamster-mouse somatic cell hybrids

1976 ◽  
Vol 2 (1) ◽  
pp. 1-10 ◽  
Author(s):  
T. Labella ◽  
G. Colletta ◽  
G. Marin
Keyword(s):  
Dna Replication ◽  
Somatic Cell ◽  
Chinese Hamster ◽  
Chromosome Loss ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Mouse Somatic Cell

Effect of Fission-Neutron Dose Rate on the Induction of a Tumor-Associated Antigen in Human Cell Hybrids (HeLa × Skin Fibroblasts)

1991 ◽  
Vol 128 (1) ◽  
pp. S71 ◽  
Author(s):  
J. L. Redpath ◽  
C. Sun ◽  
W. F. Blakely
Keyword(s):  
Dose Rate ◽  
Human Cell ◽  
Fission Neutron ◽  
Skin Fibroblasts ◽  
Neutron Dose ◽  
Cell Hybrids ◽  
Tumor Associated Antigen ◽  
Neutron Dose Rate

Dissociation of anchorage independence from tumorigenicity in human cell hybrids

1980 ◽  
Vol 26 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Eric J. Stanbridge ◽  
Joyce Wilkinson
Keyword(s):  
Human Cell ◽  
Anchorage Independence ◽  
Cell Hybrids
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