Nucleotide sequence and in vitro translation of the coat protein gene of cymbidium mosaic virus

Virus Genes ◽  
1993 ◽  
Vol 7 (2) ◽  
pp. 157-170 ◽  
Author(s):  
Koh-Koh Neo ◽  
Sek-Man Wong ◽  
Mian Wu
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
In Vitro Translation ◽  
Cymbidium Mosaic Virus

The Occurrence of Barley Mild Mosaic Virus (BaMMV) in China and the Nucleotide Sequence of its Coat Protein Gene

1999 ◽  
Vol 147 (4) ◽  
pp. 229-234 ◽  
Author(s):  
T. Zheng ◽  
Y. Cheng ◽  
J. P. Chen ◽  
J. F. Antoniw ◽  
M. J. Adams
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Mild Mosaic ◽  
Mild Mosaic Virus

scholarly journals Nucleotide sequence of the tamarillo mosaic virus coat protein gene

1990 ◽  
Vol 18 (23) ◽  
pp. 7166-7166 ◽  
Author(s):  
Robin M. Eagles ◽  
Richard C. Gardner ◽  
Richard L.S. Forster
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals Molecular cloning and nucleotide sequence of coat protein gene of turnip mosaic virus

1990 ◽  
Vol 18 (18) ◽  
pp. 5555-5555 ◽  
Author(s):  
Ling-Jie Kong ◽  
Rong-Xiang Fang ◽  
Zheng-Hua Chen ◽  
Ke-Qiang Mang
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Molecular Cloning ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Turnip Mosaic Virus

scholarly journals Biological, Pathological, and Molecular Characteristics of a New Potyvirus, Dendrobium Chlorotic Mosaic Virus, Infecting Dendrobium Orchid

Plant Disease ◽  
2019 ◽  
Vol 103 (7) ◽  
pp. 1605-1612 ◽  
Author(s):  
Chih-Hung Huang ◽  
Chia-Hsing Tai ◽  
Ruey-Song Lin ◽  
Chung-Jan Chang ◽  
Fuh-Jyh Jan
Keyword(s):  
Amino Acid ◽  
Nucleotide Sequence ◽  
Coat Protein ◽  
Amino Acid Sequence ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Nucleotide Sequence Identity ◽  
Amino Acid Sequence Identity ◽  
Protein Gene ◽  
Sequence Identity

Dendrobium smillieae is one of the popular orchids in Taiwan. This report describes a new potyvirus tentatively named Dendrobium chlorotic mosaic virus (DeCMV) causing chlorotic and mosaic symptoms in D. smillieae. Enzyme-linked immunosorbent assay (ELISA) tests using six antisera against orchid-infecting viruses revealed that only a monoclonal antibody against the potyvirus group reacted positively with crude saps prepared from a symptomatic dendrobium orchid. Potyvirus-like, flexuous, filamentous particles were observed under an electron microscope, measuring approximately 700 to 800 nm in length and 11 to 12 nm in diameter. Sequence analyses revealed that DeCMV coat protein gene shared 59.6 to 66.0% nucleotide sequence identity and 57.6 to 66.0% amino acid sequence identity, whereas the DeCMV complete genome shared 54.1 to 57.3% nucleotide sequence identity and 43.7 to 49.5% amino acid sequence identity with those other known potyviruses. These similarity levels were much lower than the criteria set for species demarcation in potyviruses. Thus, DeCMV can be considered a new potyvirus. The whole DeCMV genome contains 10,041 nucleotides (GenBank accession no. MK241979) and encodes a polyprotein that is predicted to produce 10 proteins by proteolytic cleavage. In a pathogenicity test, results of inoculation assays demonstrated that DeCMV can be transmitted to dendrobium orchids by grafting and mechanical inoculation, as verified by ELISA and western blot analyses using the DeCMV polyclonal antiserum and by reverse transcription polymerase chain reaction using the coat protein gene-specific primers. The inoculated orchids developed similar chlorotic and mosaic symptoms. In conclusion, DeCMV is a novel orchid-infecting potyvirus, and this is the first report of a new potyvirus that infects dendrobium orchids in Taiwan.

Translational in vitro activity of the 3a gene and the coat protein gene derived from brome mosaic virus RNA 3 by site-specific cleavage with RNase H

FEBS Letters ◽  
1989 ◽  
Vol 254 (1-2) ◽  
pp. 66-68 ◽  
Author(s):  
E.V. Smirnyagina ◽  
O.V. Karpova ◽  
N.A. Miroshnichenko ◽  
N.P. Rodionova ◽  
J.G. Atabekov
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Brome Mosaic Virus ◽  
Rnase H ◽  
In Vitro Activity ◽  
Site Specific ◽  
Virus Rna

Nucleotide sequence of coat protein gene of yam mild mosaic virus, isolated in Papua New Guinea

1999 ◽  
Vol 144 (7) ◽  
pp. 1415-1419 ◽  
Author(s):  
S. Fuji ◽  
I. Mitobe ◽  
H. Nakamae ◽  
K. T. Natsuaki
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Papua New Guinea ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
New Guinea ◽  
Mild Mosaic ◽  
Mild Mosaic Virus

scholarly journals The nucleotide sequence of apple mosaic virus coat protein gene has no similarity with other Bromoviridae coat protein genes

1994 ◽  
Vol 75 (10) ◽  
pp. 2847-2850 ◽  
Author(s):  
R. H. Alrefai ◽  
P. J. Shiel ◽  
L. L. Domier ◽  
C. J. D'Arcy ◽  
P. H. Berger ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals GENETIC ENGINEERING OF THE ZUCCHINI YELLOW MOSAIC VIRUS COAT PROTEIN GENE FOR EXPRESSION IN PLANTS.

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1130d-1130
Author(s):  
Guowei Fang ◽  
Rebecca Grumet
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Zucchini Yellow Mosaic Virus ◽  
In Vitro Transcription ◽  
Yellow Mosaic Virus ◽  
35S Promoter ◽  
Plant Expression Vector

Zucchini yellow mosaic virus (ZYMV), a potyvirus, can cause major losses in cucurbit crops. With the goal of genetically engineering resistance to this disease we have engineered the ZYMV coat protein gene into a plant expression vector. The complete coat protein coding sequence, or the conserved core portion of the capsid gene, was attached to the 5' untranslated region of tobacco etch virus (TEV) in the pTL37 vector (Carrington et al., 1987, Nucl. Acid Res. 15:10066) The capsid constructs were successfully expressed by in vitro transcription and translation systems as verified by SDS-PAGE and ZYMV coat protein antibody. The constructs were then subcloned using polymerase chain reaction and attached to the CaMV 35 S transcriptional promoter on the CIBA-GEIGY pCIB710 plasmid. The constructs containing the CaMV 35S promoter, the 5' untranslated leader of TEV, and ZYMV coat protein sequences were then put between the Agrobacterium tumefaciens left and right borders in the pCIB10 vector and transferred to A. tumefaciens strain LBA4404 by triparental mating. These vectors are now being used to transform muskmelon and cucumber; resultant transgenic plants will be tested for ZYMV coat protein expression.

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