PCR detection of amplified 132 bp repeats in Marek's disease virus type 1 (MDV-1) DNA can serve as an indicator for critical genomic rearrangement leading to the attenuation of virus virulence

Virus Genes ◽  
1993 ◽  
Vol 7 (3) ◽  
pp. 277-287 ◽  
Author(s):  
Yechiel Becker ◽  
Eynath Tabor ◽  
Yael Asher ◽  
Irit Davidson ◽  
Mertyn Malkinson ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Type ◽  
Marek's Disease Virus ◽  
Genomic Rearrangement ◽  
Pcr Detection ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Disease Virus Type

Protection studies against Marek's disease using baculovirus‐expressed glycoproteins B and C of Marek's disease virus type 1

1996 ◽  
Vol 25 (1) ◽  
pp. 5-24 ◽  
Author(s):  
H‐K. Jang ◽  
T. Kitazawa ◽  
M. Ono ◽  
Y. Kawaguchi ◽  
K. Maeda ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Type ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Disease Virus Type

scholarly journals Marek's Disease Virus Type 2 (MDV-2)-Encoded MicroRNAs Show No Sequence Conservation with Those Encoded by MDV-1

2007 ◽  
Vol 81 (13) ◽  
pp. 7164-7170 ◽  
Author(s):  
Yongxiu Yao ◽  
Yuguang Zhao ◽  
Hongtao Xu ◽  
Lorraine P. Smith ◽  
Charles H. Lawrie ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Type ◽  
Northern Blotting ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Repeat Region ◽  
Mirna Clusters ◽  
Disease Virus Type

ABSTRACT MicroRNAs (miRNAs) are increasingly being recognized as major regulators of gene expression in many organisms, including viruses. Among viruses, members of the family Herpesviridae account for the majority of the currently known virus-encoded miRNAs. The highly oncogenic Marek's disease virus type 1 (MDV-1), an avian herpesvirus, has recently been shown to encode eight miRNAs clustered in the MEQ and LAT regions of the viral genome. The genus Mardivirus, to which MDV-1 belongs, also includes the nononcogenic but antigenically related MDV-2. As MDV-1 and MDV-2 are evolutionarily very close, we sought to determine if MDV-2 also encodes miRNAs. For this, we cloned, sequenced, and analyzed a library of small RNAs from the lymphoblastoid cell line MSB-1, previously shown to be coinfected with both MDV-1 and MDV-2. Among the 5,099 small RNA sequences determined from the library, we identified 17 novel MDV-2-specific miRNAs. Out of these, 16 were clustered in a 4.2-kb long repeat region that encodes R-LORF2 to R-LORF5. The single miRNA outside the cluster was located in the short repeat region, within the C-terminal region of the ICP4 homolog. The expression of these miRNAs in MSB-1 cells and infected chicken embryo fibroblasts was further confirmed by Northern blotting analysis. The identification of miRNA clusters within the repeat regions of MDV-2 demonstrates conservation of the relative genomic positions of miRNA clusters in MDV-1 and MDV-2, despite the lack of sequence homology among the miRNAs of the two viruses. The identification of these novel miRNAs adds to the growing list of virus-encoded miRNAs.

scholarly journals Improving DNA Vaccine Potency by Linking Marek's Disease Virus Type 1 VP22 to an Antigen

2002 ◽  
Vol 76 (6) ◽  
pp. 2676-2682 ◽  
Author(s):  
Chien-Fu Hung ◽  
Liangmei He ◽  
Jeremy Juang ◽  
Tzyy-Jye Lin ◽  
Morris Ling ◽  
...  
Keyword(s):  
Disease Virus ◽  
Dna Vaccine ◽  
Virus Type ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Vaccine Potency ◽  
Disease Virus Type ◽  
Hsv 1

ABSTRACT We have previously employed an intercellular spreading strategy using herpes simplex virus type 1 (HSV-1) VP22 protein to enhance DNA vaccine potency because DNA vaccines lack the intrinsic ability to amplify in cells. Recently, studies have demonstrated that the protein encoded by UL49 of Marek's disease virus type 1 (MDV-1) exhibits some degree of homology to the HSV-1 VP22 protein and features the property of intercellular transport. We therefore generated a DNA vaccine encoding MDV-1 VP22 linked to a model antigen, human papillomavirus type 16 E7. We demonstrated that compared with mice vaccinated with DNA encoding wild-type E7, mice vaccinated with MDV-1 VP22/E7 DNA exhibited a significant increase in number of gamma-interferon-secreting, E7-specific CD8+-T-cell precursors as well as stronger tumor prevention and treatment effects. Furthermore, our data indicated that the antitumor effect was CD8 dependent. These results suggested that the development of vaccines encoding VP22 fused to a target antigen might be a promising strategy for improving DNA vaccine potency.

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