scholarly journals Characterization and localization of the unique Marek's disease virus type 2 ORF873 gene product

2004 ◽  
Vol 5 (3) ◽  
pp. 207 ◽  
Author(s):  
Hyung Kwan Jang
Keyword(s):  
Gene Product ◽  
Disease Virus ◽  
Virus Type ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Disease Virus Type

Protection studies against Marek's disease using baculovirus‐expressed glycoproteins B and C of Marek's disease virus type 1

1996 ◽  
Vol 25 (1) ◽  
pp. 5-24 ◽  
Author(s):  
H‐K. Jang ◽  
T. Kitazawa ◽  
M. Ono ◽  
Y. Kawaguchi ◽  
K. Maeda ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Type ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Disease Virus Type

scholarly journals Marek's Disease Virus Type 2 (MDV-2)-Encoded MicroRNAs Show No Sequence Conservation with Those Encoded by MDV-1

2007 ◽  
Vol 81 (13) ◽  
pp. 7164-7170 ◽  
Author(s):  
Yongxiu Yao ◽  
Yuguang Zhao ◽  
Hongtao Xu ◽  
Lorraine P. Smith ◽  
Charles H. Lawrie ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Type ◽  
Northern Blotting ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Repeat Region ◽  
Mirna Clusters ◽  
Disease Virus Type

ABSTRACT MicroRNAs (miRNAs) are increasingly being recognized as major regulators of gene expression in many organisms, including viruses. Among viruses, members of the family Herpesviridae account for the majority of the currently known virus-encoded miRNAs. The highly oncogenic Marek's disease virus type 1 (MDV-1), an avian herpesvirus, has recently been shown to encode eight miRNAs clustered in the MEQ and LAT regions of the viral genome. The genus Mardivirus, to which MDV-1 belongs, also includes the nononcogenic but antigenically related MDV-2. As MDV-1 and MDV-2 are evolutionarily very close, we sought to determine if MDV-2 also encodes miRNAs. For this, we cloned, sequenced, and analyzed a library of small RNAs from the lymphoblastoid cell line MSB-1, previously shown to be coinfected with both MDV-1 and MDV-2. Among the 5,099 small RNA sequences determined from the library, we identified 17 novel MDV-2-specific miRNAs. Out of these, 16 were clustered in a 4.2-kb long repeat region that encodes R-LORF2 to R-LORF5. The single miRNA outside the cluster was located in the short repeat region, within the C-terminal region of the ICP4 homolog. The expression of these miRNAs in MSB-1 cells and infected chicken embryo fibroblasts was further confirmed by Northern blotting analysis. The identification of miRNA clusters within the repeat regions of MDV-2 demonstrates conservation of the relative genomic positions of miRNA clusters in MDV-1 and MDV-2, despite the lack of sequence homology among the miRNAs of the two viruses. The identification of these novel miRNAs adds to the growing list of virus-encoded miRNAs.

Sign in / Sign up

Export Citation Format

Share Document