Detection ofClostridium difficile toxin by enzyme immunoassay, tissue culture test and culture

Two techniques for rapid diagnosis, immunofluorescence (IFAT) and enzyme immunoassay (EIA), have been compared with virus isolaion in tissue culture for the detection of respiratory syncytial virus (RSV) in specimens of nasopharyngeal secretions. The specimens were obtained from children under five years of age suffering from acute respiratory iliness, during a period of six months from January to June 1982. Of 471 specimens examined 54 (11.5%) were positive by virus isolation and 180 (38.2%) were positive by immunofluorescence. The bacterial contamination of inoculated tissue cultures unfortunately prevented the isolation of virus from many samples. Specimens from 216 children were tested to compare enzyme immunoassay and immunofluorescence. Of these 60 (27%) were positive by EIA and 121 (56%) were positive by IFAT. Our results suggest that the EIA technique although highly specific is rather insensitive. This may be because by the time these tests were done the originl nasopharyngeal secretions were considerably diluted and contained more mucus fragments than the call suspension used for IFAT. Of the three techniques, IFAT gives the best results although EIA may be useful where IFAT is not possible.

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Rapid, Direct Tissue Culture Test for Toxigenicity of Corynebacterium Diphtheriae

Applied Microbiology ◽

10.1128/aem.25.5.709-712.1973 ◽

1973 ◽

Vol 25 (5) ◽

pp. 709-712 ◽

Cited By ~ 9

Author(s):

Walter Laird ◽

Neal Groman

Keyword(s):

Tissue Culture ◽

Corynebacterium Diphtheriae ◽

Culture Test

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Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture.

Journal of Clinical Microbiology ◽

10.1128/jcm.21.1.88-91.1985 ◽

1985 ◽

Vol 21 (1) ◽

pp. 88-91 ◽

Cited By ~ 7

Author(s):

A Merli ◽

A Canessa ◽

G Melioli

Keyword(s):

Tissue Culture ◽

Toxoplasma Gondii ◽

Enzyme Immunoassay

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Discrepancies in carcinoembryonic antigen measurements: survey and control values vs values for patients.

Clinical Chemistry ◽

10.1093/clinchem/33.4.563 ◽

1987 ◽

Vol 33 (4) ◽

pp. 563-566 ◽

Cited By ~ 3

Author(s):

G G Klee ◽

L A Dodge ◽

G Reynoso

Keyword(s):

Tissue Culture ◽

Carcinoembryonic Antigen ◽

Enzyme Immunoassay ◽

Colon Carcinoma ◽

Proficiency Testing ◽

Analytical Methods ◽

Test Results ◽

And Control ◽

The Relationship ◽

Metastatic Colon Carcinoma

Abstract We analyzed the carcinoembryonic antigen (CEA) test results reported in the College of American Pathologists' (CAP) surveys to determine the relationship between the source of CEA used to manufacture the survey specimens and the discrepancies among analytical methods. With the 1983 survey specimens, which were prepared from metastatic colon carcinoma, laboratories using Roche RIA with Clinetics columns reported results that were only one-half the values reported by laboratories using the Abbott polyclonal enzyme immunoassay. With the 1984 specimens, prepared from a different metastatic colon carcinoma, and the 1985 specimens, prepared from a tissue-culture source of CEA, the Roche results were about one-sixth as large as the Abbott results. These differences are larger than the reported assay differences for patients' specimens. In addition, twofold proportional differences were found when survey and control specimens were tested with different lots of Abbott polyclonal reagent, whereas only random differences were found with 102 patients' specimens. Evidently, assay systems perform differently with proficiency-testing and control specimens than with patients' specimens.

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