The changes of interleukin-2, tumour necrotic factor and gamma-interferon production among patients with Kawasaki disease

1991 ◽  
Vol 150 (3) ◽  
pp. 179-182 ◽  
Author(s):  
C. -Y. Lin ◽  
C. -C. Lin ◽  
B. Hwang ◽  
B. N. Chiang
Keyword(s):  
Kawasaki Disease ◽  
Interleukin 2 ◽  
Gamma Interferon ◽  
Interferon Production ◽  
Tumour Necrotic Factor

scholarly journals Recombinant Mycobacterium bovis BCG secreting functional interleukin-2 enhances gamma interferon production by splenocytes.

1994 ◽  
Vol 62 (6) ◽  
pp. 2508-2514 ◽  
Author(s):  
M A O'Donnell ◽  
A Aldovini ◽  
R B Duda ◽  
H Yang ◽  
A Szilvasi ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Interleukin 2 ◽  
Gamma Interferon ◽  
Interferon Production ◽  
Mycobacterium Bovis Bcg

Inhibitory Effects of Histamine on Interleukin 2 and Gamma Interferon Production by Different Human T Helper Cell Subsets

1988 ◽  
Vol 28 (6) ◽  
pp. 727-733 ◽  
Author(s):  
M. DOHLSTEN ◽  
G. HEDLUND ◽  
H.-O. SJOGREN ◽  
R. CARLSSON
Keyword(s):  
Interleukin 2 ◽  
Gamma Interferon ◽  
T Helper Cell ◽  
Helper Cell ◽  
Interferon Production ◽  
T Helper ◽  
Inhibitory Effects

scholarly journals Phorbol ester replacement of helper cell and interleukin 2 requirements in gamma interferon production.

1982 ◽  
Vol 36 (3) ◽  
pp. 911-914 ◽  
Author(s):  
H M Johnson ◽  
B A Torres
Keyword(s):  
Phorbol Ester ◽  
Interleukin 2 ◽  
Gamma Interferon ◽  
Helper Cell ◽  
Interferon Production

scholarly journals IMMUNOGENIC PROPERTIES OF RECOMBINANT MOZAIC PROTEINS BASED ON ANTIGENS NS4A AND NS4B OF HEPATITIS C VIRUS

2018 ◽  
Vol 63 (3) ◽  
pp. 138-143
Author(s):  
V. V. Koupriyanov ◽  
L. I. Nikolaeva ◽  
A. A. Zykova ◽  
P. I. Makhnovskiy ◽  
R. Y. Kotlyarov ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Recombinant Protein ◽  
Recombinant Proteins ◽  
Core Protein ◽  
Interleukin 2 ◽  
Gamma Interferon ◽  
Interferon Production ◽  
Helper Epitope ◽  
Immunogenic Properties

The aim of the study was to investigate immunogenic properties of mosaic recombinant proteins constructed on the data of hepatitis C virus NS4A and NS4B antigens. Four mosaic recombinant proteins, containing the T and B epitopes of the NS4A and NS4B antigens, were created by genetic engineering methods in the E. coli system. To enhance the immune response they were linked in different variations to the nucleotide sequences of murine interleukin-2 (IL-2), the Neisseria meningiditis lipopeptide, and the T helper epitope of the core protein of hepatitis C virus. The immunogenic properties of these recombinant proteins were analyzed by immunoblotting, ELISA and ELISpot using sera from immunized mice and patients infected with hepatitis C virus. Recombinant proteins specifically reacted with the sera of immunized mice and infected patients in immunoblotting. According to the ELISA data, the predominant formation of antibodies to NS4B was observed when mice were immunized with the recombinant proteins containing both antigens. Analysis of gamma-interferon production by T-lymphocytes upon contact with activated dendritic cells showed in ELISpot that the maximum production of this cytokine was detected when adjuvant components were located at the N- and C-ends of the recombinant protein. The highest level of gamma-interferon production during stimulation with this drug was detected in lymphocytes from the bone marrow and lymph nodes. The recombinant protein containing the T and B epitopes of NS4A and NS4B, murine IL-2 and the lipopeptide Neisseria meningiditis had the greatest immunostimulate effect among the four constructions. This recombinant protein formed nanoparticles of 100-120 nm in size.

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