Changes in guinea pig lung beta-adrenoceptor function byHaemophilus influenzae and its mediation by specifically stimulated pulmonary macrophages

1986 ◽  
Vol 17 (3-4) ◽  
pp. 401-402 ◽  
Author(s):  
Ferdi Engels ◽  
Ronald S. Oosting ◽  
Frans P. Nijkamp
Keyword(s):  
Guinea Pig ◽  
Beta Adrenoceptor ◽  
Pulmonary Macrophages ◽  
Byhaemophilus Influenzae

Comparative physiology of rodent pulmonary macrophages: in vitro functional responses

1988 ◽  
Vol 64 (5) ◽  
pp. 1953-1959 ◽  
Author(s):  
D. B. Warheit ◽  
M. A. Hartsky ◽  
M. S. Stefaniak
Keyword(s):  
Guinea Pig ◽  
Phagocytic Activity ◽  
Lung Cells ◽  
Comparative Physiology ◽  
Functional Responses ◽  
Inhaled Particles ◽  
Pulmonary Macrophages ◽  
The One ◽  
Formyl Peptides

Since toxicological testing of inhaled materials frequently requires utilization of several species, we have investigated pulmonary macrophage (PM) functional responses and compared the rat model with other rodents. Two strains of rats, three strains of mice, and one strain each of hamster and guinea pig were used in this study. The numbers of recovered cells by bronchoalveolar lavage generally correlated with animal body weight. The one exception was the Syrian Golden hamster from which increased numbers of macrophages were recovered. Cellular differential data obtained from lavaged cytocentrifuge preparations demonstrated that PM's account for greater than 97% of recoverable free lung cells for all species except the guinea pig, which contains a resident population of eosinophils. Cell morphology studies indicated that hamster PM exhibited the highest proportion of ruffled PM and demonstrated the highest phagocytic activity, whereas mouse PM phagocytic activity was significantly reduced compared with the other three species. In addition, chemotaxis studies showed that rat PM migrated best to zymosan-activated, complement-dependent chemoattractants, whereas hamster PM demonstrated an enhanced chemotactic response to N-formyl peptides. The results of these studies suggest that the rat may be the most efficient species for clearing inhaled particles, whereas hamsters and guinea pigs may best respond to bacteria.

scholarly journals Effects of Catecholamine Metabolites on Beta-Adrenoceptor-Mediated Relaxation of Smooth Muscle: Evaluation in Mouse and Guinea-Pig Trachea and Rat Aorta

2020 ◽  
Vol 43 (3) ◽  
pp. 493-502
Author(s):  
Fumiko Yamaki ◽  
Anna Koike ◽  
Hikari Kono ◽  
Xiaoyue Zhang ◽  
Kento Yoshioka ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Rat Aorta ◽  
Beta Adrenoceptor ◽  
Catecholamine Metabolites

Adrenergic-cholinergic interactions on membrane potential of K+ -depolarized ventricular muscle

1984 ◽  
Vol 247 (2) ◽  
pp. H244-H250
Author(s):  
T. Ehara ◽  
T. Mitsuiye
Keyword(s):  
Membrane Potential ◽  
Guinea Pig ◽  
Induced Response ◽  
Channel Blockers ◽  
Ventricular Muscle ◽  
Tyrode Solution ◽  
Channel System ◽  
Beta Adrenoceptor ◽  
Slow Channel ◽  
Rebound Increase

In guinea pig ventricular muscles exposed to K+-rich (27 mM) Tyrode solution containing 0.2 mM Ba, catecholamine elicited a slight depolarization of the resting membrane. Application of acetylcholine (ACh) during this catecholamine-induced response caused a repolarization, and removal of ACh induced a transient enhancement in the depolarization (rebound). These effects of ACh were abolished by atropine. Application of ACh alone and its removal had little effect on the membrane potential. Like the catecholamine-induced depolarization, the rebound depolarization after ACh removal was inhibited by slow channel blockers. Thus the rebound was attributed at least in part to enhanced changes in the catecholamine-sensitive conductance, i.e., a beta-receptor-mediated increase in the slow channel conductance. In driven muscles perfused with normal Tyrode solution, there was a rebound increase in twitch tension when ACh was removed in the presence of catecholamine, and this rebound was accompanied by an "extra" elevation of the action potential plateau. Thus cessation of the stimulation of myocardial muscarinic receptors may transiently lead to an enhanced activity of the beta-adrenoceptor-slow channel system in guinea pig ventricular muscle.

scholarly journals Cyclic AMP-evoked oscillations of intracellular [Ca2+] in guinea-pig hepatocytes

1991 ◽  
Vol 275 (1) ◽  
pp. 277-280 ◽  
Author(s):  
T Capiod ◽  
J Noel ◽  
L Combettes ◽  
M Claret
Keyword(s):  
Angiotensin Ii ◽  
Cyclic Amp ◽  
Guinea Pig ◽  
Intracellular Perfusion ◽  
Beta Adrenoceptor ◽  
Adrenoceptor Agonist ◽  
Patch Pipette

The effects of the beta-adrenoceptor agonist isoprenaline and cyclic AMP (cAMP) on cytosolic free Ca2+ ([Ca2+]i) were studied in the single guinea-pig hepatocyte. In common with InsP3-dependent agonists such as noradrenaline or angiotensin II, isoprenaline (0.5-10 microM) and cAMP (50-100 mM, perfused into the cell via the patch-pipette), were able to generate fast and slow fluctuations of [Ca2+]i. Responses to isoprenaline and cAMP also were observed in the absence of external Ca2+. Isoprenaline-evoked [Ca2+]i rises were not blocked by the intracellular perfusion of heparin, suggesting that these fluctuations are independent of the binding of InsP3 to its receptor.

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