Anomalous responses to histamine stimulation of guinea-pig oesophageal muscularis mucosae

1. A DNA radioimmunoassay, sensitive in the range 25–1000 ng, has been developed to measure gastric mucosal cell loss. Validity of the assay was based on antibody specificity, absence of interference from gastric contents, parallel tracer displacement by dilutions of gastric and standard DNA, and crossover with colorimetric assay. 2. With this assay, gastric DNA shedding was examined in two animal species and man. In the guinea-pig stomach, DNA loss was 20–100 pg/h and in the canine Pavlov pouch, 260–580pg/h. In the canine Pavlov pouch stimulation of acid secretion by histamine and exposure to exogenous acid increased DNA output. In the normal human stomach DNA loss was 544 k 175 pg/h and in duodenal ulcer patients, 649 k 225 pg/li. 3. DNA radioimmunoassay provides a sensitive and reproducible measure of cell exfoliation from the stomach and may be a useful tool for studying aspects of gastric mucosal defence.

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Effects of Electrical Stimulation of the Superior Cervical Ganglion on Cochlear Blood Flow in Guinea Pig

Acta Oto-Laryngologica ◽

10.3109/00016489309135783 ◽

1993 ◽

Vol 113 (2) ◽

pp. 146-151 ◽

Cited By ~ 31

Author(s):

Tian-Ying Ren ◽

E. Laurikainen ◽

W. S. Quirk ◽

J. M. Miller ◽

A. L. Nuttall

Keyword(s):

Blood Flow ◽

Electrical Stimulation ◽

Guinea Pig ◽

Superior Cervical Ganglion ◽

Cochlear Blood Flow ◽

Cervical Ganglion ◽

Stimulation Of

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Turnover of protein-bound serine phosphate in respiring slices of guinea-pig cerebral cortex. Effects of putative transmitters, tetrodotoxin and other agents

Biochemical Journal ◽

10.1042/bj1320475 ◽

1973 ◽

Vol 132 (3) ◽

pp. 475-482 ◽

Cited By ~ 30

Author(s):

Martin Reddington ◽

Richard Rodnight ◽

Michael Williams

Keyword(s):

Cerebral Cortex ◽

Cyclic Amp ◽

Guinea Pig ◽

Electrical Pulses ◽

Phosphorus Turnover ◽

Serine Phosphate ◽

Stimulation Of

1. The effect of various agents on the turnover of protein-bound phosphorus in respiring slices of cerebral cortex was studied. 2. Confirming previous work turnover was increased by the application of electrical pulses for 10s to the tissue. 3. Turnover was also increased by exposure of the slices for 10min to noradrenaline (0.5mm), 5-hydroxytryptamine (1μm) and histamine (0.1mm). 4. When slices were stimulated by electrical pulses in the presence of histamine the increase in turnover was the sum of the responses given by each agent above, suggesting that different phosphorylating systems were involved. 5. Tetrodotoxin (0.5μm) blocked the increased turnover due to electrical pulses, but not that due to histamine. Tetrodotoxin also prevented the increase in tissue cyclic AMP content caused by the application of electrical pulses. 6. Phosphoprotein turnover was not affected by adenosine, despite the increase in tissue cyclic AMP content given by this agent. 7. Adenosine blocked the phosphoprotein response to histamine, but did not affect the response to electrical pulses. 8. The results are discussed in relation to the hypothesis that the stimulation of protein phosphorus turnover by electrical pulses is secondary to the release of cyclic AMP in the tissue.

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