[3H]Formate incorporation into liver nucleic acids of chick. Relationships between orotic acid and vitamin B12

1967 ◽  
Vol 23 (7) ◽  
pp. 521-522 ◽  
Author(s):  
C. M. Caldarera ◽  
B. Barbiroli ◽  
M. Marchetti
Keyword(s):  
Nucleic Acids ◽  
Vitamin B12 ◽  
Orotic Acid

Insulin and incorporation of radioactivity into nucleic acid fraction of isolated diaphragm

1960 ◽  
Vol 199 (4) ◽  
pp. 719-721 ◽  
Author(s):  
Ira G. Wool
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Glucose Concentration ◽  
Orotic Acid ◽  
Acid Fraction ◽  
Rat Diaphragm ◽  
Isolated Rat

Insulin in vitro stimulated the incorporation into the nucleic acid fraction of isolated rat diaphragm of radioactivity from d-glucose-U-C14, adenine-8-C14 and orotic acid-6-C14; insulin had no effect on the incorporation of thymine-2-C14 into muscle nucleic acids. Insulin enhanced the incorporation into nucleic acids of C14 from adenine and orotic acid in the absence of added glucose, and incorporation of adenine-8-C14 was not influenced by glucose concentration over the range 0–600 mg %.

scholarly journals Effect of denervation on the synthesis of ribonucleic acid and deoxyribonucleic acid in rat diaphragm muscle

1968 ◽  
Vol 108 (2) ◽  
pp. 177-183 ◽  
Author(s):  
K L Manchester ◽  
E J Harris
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Orotic Acid ◽  
Deoxyribonucleic Acid ◽  
Diaphragm Muscle ◽  
Unit Mass ◽  
Rat Diaphragm ◽  
The Third

1. The effect of unilateral denervation of rat diaphragm muscle on its content of nucleic acids and their incorporation of precursors was investigated. 2. After denervation the paralysed hemidiaphragm hypertrophies and within 3 days its content of RNA increases considerably. The concentration of DNA/unit mass remains fairly constant. 3. During this period there is some increase in the rate of incorporation of [14C]adenine into RNA, whereas there is some diminution in the rate of incorporation of [14C]orotic acid. 4. Incorporation of [14C]adenine and [3H]thymidine into DNA is much increased in the paralysed tissue, reaching its maximum by about the third day, but returning to normal by the tenth. 5. The significance of these results in relation to the hypertrophy after denervation is discussed.

Effects of orotic acid and methionine on acid-soluble nucleotides of vitamin B12-deficient chicks

1962 ◽  
Vol 55 (1-2) ◽  
pp. 218-220 ◽  
Author(s):  
M. Marchetti ◽  
C.M. Caldarera ◽  
G. Moruzzi
Keyword(s):  
Vitamin B12 ◽  
Orotic Acid

ChemInform Abstract: Vitamin B12-Derivatives - Enzyme Cofactors and Ligands of Proteins and Nucleic Acids

ChemInform ◽  
2011 ◽  
Vol 42 (45) ◽  
pp. no-no
Author(s):  
Karl Gruber ◽  
Barbara Puffer ◽  
Bernhard Kraeutler
Keyword(s):  
Nucleic Acids ◽  
Vitamin B12 ◽  
Enzyme Cofactors

Pyrimidine metabolism in seeds and seedlings of jack pine (Pinus banksiana)

1975 ◽  
Vol 53 (7) ◽  
pp. 673-686 ◽  
Author(s):  
J. Pitel ◽  
D. J. Durzan
Keyword(s):  
Nucleic Acids ◽  
Dna Synthesis ◽  
Pinus Banksiana ◽  
Orotic Acid ◽  
De Novo ◽  
Jack Pine ◽  
Pyrimidine Metabolism ◽  
De Novo Synthesis ◽  
Pine Seedlings ◽  
Low Levels

Jack pine seedlings rapidly converted radioactive thymine-6-3H to dihydrothymine, β-ureidoisobutyric acid, and β-aminoisobutyric acid. Autoradiographs of hypocotyl cells showed radioactivity largely in the cytoplasm. A small percentage of radioactivity from thymine-2-14C was salvaged via uracil for the synthesis of RNA. DNA was not labelled significantly. The occurrence of a mechanism to utilize thymidine-methyl-3H for DNA synthesis was detected at low levels in germinating seedlings but was absent in seeds up to 16 h imbibition. Among pyrimidines tested thymidine was the most specific for the labelling of DNA and radioactivity was detected almost entirely over nuclei.The occurrence of an active pathway for the de novo synthesis of the pyrimidine portions of nucleic acids was demonstrated by the use of orotic acid-4-14C. Native DNA and all major types of RNA were labelled and radioactivity resided in pyrimidines. Synthesis of DNA involved the conversion of pyrimidine ribonucleotides to deoxyribonucleotides.

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