Polyploidization in leaf callus tissue and in regenerated plants of dihaploid potato

1981 ◽  
Vol 1 (1) ◽  
pp. 77-84 ◽  
Author(s):  
E. Jacobsen
Keyword(s):  
Callus Tissue ◽  
Regenerated Plants ◽  
Dihaploid Potato

scholarly journals Production of Procyanidin compound in seeds , callus and in regenerated plants from vitis vinifera L.

2010 ◽  
Vol 4 (2) ◽  
pp. 76-83
Author(s):  
Karem T. K. AL-Hatemy
Keyword(s):  
Vitis Vinifera ◽  
High Performance ◽  
Callus Tissue ◽  
Mother Plant ◽  
Vitis Vinifera L ◽  
Ms Medium ◽  
Grape Seeds ◽  
Extraction And Purification ◽  
Regenerated Plants ◽  
Regenerated Plant

This experiment was conducted in Science and Agriculture College labs, Kufa University, on 2008 to describe methods for extraction and purification of Procyanidin compound from each of grape seeds mother plant(Vitis vinifera L.) ,callus induced from Axillary buds by using MS medium supplemented with 2,4-D at concentration of (1,2,3)mg/l and from leaves of regenerated from callus by using MS medium supplemented with ( IAA )at concentration of 2mg/l and (BA) benzyl adenine at concentration of 0.2 mg/l and from cocoas powdered (control) as well these Procyanidin. The content of Procyanidin were done by using HPLC (high performance liquid chromatography) and compare of the quantitatively of Procyanidin with these seeds in mother plant and callus tissue. This study include alcohol extraction by using mixture of ethanol, methanol, D.W and HCL. The result revealed that, the superiority of Procyanidin in seeds content than the content in callus and regenerated plant and increase the content of these compound in the leaves of regenerated plant with the content in callus tissue.

scholarly journals In vitro genetic variability of sugarcane cultivars

1969 ◽  
Vol 85 (1-2) ◽  
pp. 49-61
Author(s):  
Asia Yusely Zambrano ◽  
Jhonny R. Demey ◽  
V. González R.
Keyword(s):  
Genetic Variability ◽  
Genetic Stability ◽  
Callus Tissue ◽  
Cell Suspensions ◽  
Regenerated Plants ◽  
High Uniformity ◽  
Genotypic Stability ◽  
Isoenzymatic Pattern

Genotypic stability and uniformity of field plants from sugarcane cultivars grown in vitro (calli and cell suspensions) were evaluated by isoenzymatic peroxidase analysis after regeneration, Phenotypes of field plants exhibited uniformity within cultivars and differences among cultivars. Distribution of PRX isoenzymes was different in the constitution of the calli phenotypes, not only among cultivars but also within the same cultivar. Genotype PR980 showed a 60% heterogeneity coefficient, whereas the call of cultivars PR62258, PR641791, PR692176, V64-10 and V71-51 showed identity coefficient of 100%. The isoenzymatic pattern of PRX for the phenotypes of cell suspensions was different among the four cuitivars used, as well as within cultivars PR980 and V64-10, all of which showed heterogeneity coefficients of 50 and 22%, respectively. Nevertheless, the identity coefficient for regenerated plants of all genotypes was 100%. In sugarcane, the isoenzyme PRX1 is related to differentiated tissue, since it is present only in phenotypes from field and regenerated plants. PRX4 is induced in vitro conditions since it was observed only in callus tissue and ceil suspensions,This study detected high uniformity and genetic stability within the regenerated plants in 100% of the sugarcane cultivars used.

scholarly journals Callus culture and micropropagation of Eustoma grandiflorum Shinn.

2014 ◽  
Vol 57 (4) ◽  
pp. 423-430 ◽  
Author(s):  
Lutosława Skrzypczak ◽  
Maria Wesołowska ◽  
Barbara Thiem
Keyword(s):  
North America ◽  
Medicinal Plant ◽  
Callus Culture ◽  
Embryo Culture ◽  
Callus Tissue ◽  
Eustoma Grandiflorum ◽  
Regenerated Plants ◽  
Short Time ◽  
Eustoma Grandiflorum Shinn

Regenerated plants and callus tissue were obtained by embryo culture and micropropagation of explants from shoots and leaves of <em>Eustoma grandiflorum</em> Shinn. (<em>Gentianaceae</em>), a medicinal plant. This species, which does not naturally occur in this country, but is found in the southwestern regions of North America, can be propagated in vitro in a relatively short time. Regenerated plants were obtained in amounts enabling extensive phytochemical analyses to be conducted.

scholarly journals In vitro cultures of Blackstonia perfoliata (L.) Hudson and an assay of the secoiridoid content

2014 ◽  
Vol 61 (3-4) ◽  
pp. 359-368 ◽  
Author(s):  
Lutosława Skrzypczak ◽  
Maria Wesołowska ◽  
Anna Krupińska ◽  
Barbara Thiem
Keyword(s):  
Medicinal Plant ◽  
Callus Tissue ◽  
In Vitro Cultures ◽  
Regenerated Plants

<i>In vitro</i> cultures of <i>Blackstonia perfoliata</i> (L.) Hudson, a popular medicinal plant, were initiated. Regenerated plants and callus tissue were obtained and then analysed for secoiridoids.

scholarly journals Micropropagation of Catalpa bignonioides Walt. through tissue cultures

2014 ◽  
Vol 58 (2) ◽  
pp. 185-198 ◽  
Author(s):  
Halina Wysokińska ◽  
Lucjan Świątek
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Tissue ◽  
Solid Medium ◽  
Tissue Cultures ◽  
Regenerated Plants ◽  
Further Development

The conditions used in the micropropagation of <em>Catalpa bignonioides </em>Walt. <em>(Bignoniaceae) </em>from callus tissue are described. The multiplication of shoots was best on Schenk and Hildebrandt (SH) solid medium supplemented with 0.5mg dm<sup>-3</sup> indolil-3-acetic acid (IAA) and 2.0 mg dm<sup>-3</sup> benzylaminopurine (BAP). Under these conditions, 11 shoots per culture could be developed within 4 weeks. To develop roots, the shoots were then transferred to basal SH medium without growth regulators. Rooted plantlets were obtained within 2-8 weeks. Regenerated plants were fully capable of further development in soil. Generally, ca 10 plants could be obtained from a fragment of callus within 10-12 weeks.

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