scholarly journals Callus culture and micropropagation of Eustoma grandiflorum Shinn.

2014 ◽  
Vol 57 (4) ◽  
pp. 423-430 ◽  
Author(s):  
Lutosława Skrzypczak ◽  
Maria Wesołowska ◽  
Barbara Thiem
Keyword(s):  
North America ◽  
Medicinal Plant ◽  
Callus Culture ◽  
Embryo Culture ◽  
Callus Tissue ◽  
Eustoma Grandiflorum ◽  
Regenerated Plants ◽  
Short Time ◽  
Eustoma Grandiflorum Shinn

Regenerated plants and callus tissue were obtained by embryo culture and micropropagation of explants from shoots and leaves of <em>Eustoma grandiflorum</em> Shinn. (<em>Gentianaceae</em>), a medicinal plant. This species, which does not naturally occur in this country, but is found in the southwestern regions of North America, can be propagated in vitro in a relatively short time. Regenerated plants were obtained in amounts enabling extensive phytochemical analyses to be conducted.

scholarly journals In vitro cultures of Blackstonia perfoliata (L.) Hudson and an assay of the secoiridoid content

2014 ◽  
Vol 61 (3-4) ◽  
pp. 359-368 ◽  
Author(s):  
Lutosława Skrzypczak ◽  
Maria Wesołowska ◽  
Anna Krupińska ◽  
Barbara Thiem
Keyword(s):  
Medicinal Plant ◽  
Callus Tissue ◽  
In Vitro Cultures ◽  
Regenerated Plants

<i>In vitro</i> cultures of <i>Blackstonia perfoliata</i> (L.) Hudson, a popular medicinal plant, were initiated. Regenerated plants and callus tissue were obtained and then analysed for secoiridoids.

Genetic stability and phytochemical profiling of the in vitro regenerated plants of Angelica glauca Edgew.: an endangered medicinal plant of Himalaya

2018 ◽  
Vol 135 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Janhvi Mishra Rawat ◽  
Aakriti Bhandari ◽  
Susmita Mishra ◽  
Balwant Rawat ◽  
Ashok Kumar Dhakad ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Genetic Stability ◽  
Regenerated Plants ◽  
Endangered Medicinal Plant ◽  
Angelica Glauca

scholarly journals Callus Culture of Arceuthobium americanum Nutt. ex Englem. and Explant Response to Varying Auxin and Cytokinin Concentrations

2012 ◽  
Vol 21 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Lyssa L. Martin ◽  
Cynthia Ross Friedman ◽  
Ronald G. Smith
Keyword(s):  
North America ◽  
Callus Culture ◽  
Shoot Organogenesis ◽  
Coniferous Forests ◽  
Western North America ◽  
Laboratory Studies ◽  
Dwarf Mistletoe ◽  
Arceuthobium Americanum ◽  
Infected Area

The lodgepole pine dwarf mistletoe Arceuthobium americanum is a severe pest in coniferous forests of western North America.  To facilitate laboratory studies of this obligate parasite, a modified White’s medium was used for in vitro culture; the concentrations of IAA and Kn were varied to determine optimal IAA/Kn ratios.  It was found that explant health was related to the concentration of IAA (p = 0.008), but not Kn (p = 0.937), and that explants were healthiest at an IAA/Kn ratio of 0.1.  Radicular apices were generated at IAA/Kn ratios of 1.0 or greater, but no shoot organogenesis was observed.  These data suggest that A. americanum is more sensitive to auxins than cytokinins.  Parasites of plants are known to secrete high levels of cytokinins, which stimulate the host to shuttle nutrients to the infected area, and so we suspect that A. americanum has reduced cytokinin sensitivity.   Key words: Callus culture, Arceuthobium americanum, Explant, Response, Cytokinin, Insensitivity   D.O.I. 10.3329/ptcb.v21i1.9557   Plant Tissue Cult. & Biotech. 21(1): 1-10, 2011 (June)

scholarly journals Physiological and biochemical effects of morphactin IT 3233 on callus and tumour tissues of Nicotiana tabacum L. cultured in vitro. IV. IAA oxidase activity. Oat coleoptile biotest

2015 ◽  
Vol 43 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Z. Chirek
Keyword(s):  
Oxidase Activity ◽  
Callus Tissue ◽  
Tissue Growth ◽  
Tumour Tissue ◽  
Iaa Oxidase ◽  
Nicotiana Tabacum L ◽  
Coleoptile Elongation ◽  
Short Time ◽  
Physiological And Biochemical

IAA oxidase activity in callus and tumour tissue of tobacco subjected to the action of morphactin IT 3233 for shorter and longer periods was determined. Control tumour tissue shows an activity higher by about 40 per cent as compared with that of callus tissue. Morphactin applied for a short time (24-h incubation) does not change the activity of the enzyme. When application is prolonged, a considerable enhancement (up to 140%) of the enzyme activity in callus tissue is observed in dependence on the morphactin concentration. In tumour tissues the activity is stimulated by 45 per cent as compared to control. Oat coleoptile elongation growth induced by IAA is limited to 40 per cent when morphactin is added in the concentrations used for tobacco tissue cultures. The possibility of the morphactin action on tissue growth via IAA metabolism is discussed.

A MICROPROPAGATION TECHNIQUE OF HEMIDESMUS INDICUS (ASCLEPIADACEAE),A VALUABLE MEDICINAL PLANT

2018 ◽  
Vol 24 (1) ◽  
Author(s):  
SUSANTA KUMAR MAITY
Keyword(s):  
Medicinal Plant ◽  
Leaf Shape ◽  
Basal Medium ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Hemidesmus Indicus ◽  
Regenerated Plants ◽  
Nodular Callus ◽  
In Vitro Clonal Propagation

An efficient protocol was established for in vitro clonal propagation of Hemidesmus indicus (Anantamul) belongs to the family Asclepiadaceae, a widely used medicinal plant through callus culture in using nodal segment. Yellowish nodular callus was observed from nodal segments on MS basal medium supplemented with 0.5 mg/L BAP + 0.2 mg/L NAA within four weeks of culture. Large number of shoots (11.4±0.2) and roots (8.2±0.4) were obtained when the callus was sub cultured on MS medium with 0.2 mg/L BAP. The regenerated plantlets were acclimatized by transferring them to soil. The survival rate of plantlets was found to be 90%. Regenerated plants were morphologically comparable having normal leaf shape and growth.

EFFECTS OF DIFFERENT PHYSIOCHEMICAL FACTORS ON REGENERATION OF CYMBOPOGON POLYNEUROS STAPF VIA CALLUS CULTURE AND SUBSEQUENT CHROMOSOMAL STABILITY

1999 ◽  
Vol 47 (3) ◽  
pp. 195-198
Author(s):  
Anath Bandhu Das
Keyword(s):  
Callus Culture ◽  
High Frequency ◽  
Potassium Salt ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Naphthalene Acetic Acid ◽  
Leaf Base ◽  
Regenerated Plants ◽  
The Individual

In vitro regeneration of Cymbopogon polyneuros Stapf was obtained through callus culture using leaf base, node, and root as explants. Callus was induced from different explants with 2–5 mg/1 α-naphthalene acetic acid (NAA) and 1–2 mg/1 kinetin in Murashige and Skoog's (MS) basal medium. High frequency shoots were noticed from leaf-base callus supplemented with 3.5 mg/1 6-benzylaminopurine (BA), L-arginine, adenine, and a low level of NAA (0.2 mg/1). About 80–85 shoot buds were obtained from ca. 200 mg of callus per culture. The individual shoots produced root in the presence of 0.5–3 mg/1 indole 3-butyric acid or its potassium salt. Regenerated plants were cytologically and phenotypically stable. Regenerants were transplanted into soil and subsequently transferred to the field.

scholarly journals In vitro genetic variability of sugarcane cultivars

1969 ◽  
Vol 85 (1-2) ◽  
pp. 49-61
Author(s):  
Asia Yusely Zambrano ◽  
Jhonny R. Demey ◽  
V. González R.
Keyword(s):  
Genetic Variability ◽  
Genetic Stability ◽  
Callus Tissue ◽  
Cell Suspensions ◽  
Regenerated Plants ◽  
High Uniformity ◽  
Genotypic Stability ◽  
Isoenzymatic Pattern

Genotypic stability and uniformity of field plants from sugarcane cultivars grown in vitro (calli and cell suspensions) were evaluated by isoenzymatic peroxidase analysis after regeneration, Phenotypes of field plants exhibited uniformity within cultivars and differences among cultivars. Distribution of PRX isoenzymes was different in the constitution of the calli phenotypes, not only among cultivars but also within the same cultivar. Genotype PR980 showed a 60% heterogeneity coefficient, whereas the call of cultivars PR62258, PR641791, PR692176, V64-10 and V71-51 showed identity coefficient of 100%. The isoenzymatic pattern of PRX for the phenotypes of cell suspensions was different among the four cuitivars used, as well as within cultivars PR980 and V64-10, all of which showed heterogeneity coefficients of 50 and 22%, respectively. Nevertheless, the identity coefficient for regenerated plants of all genotypes was 100%. In sugarcane, the isoenzyme PRX1 is related to differentiated tissue, since it is present only in phenotypes from field and regenerated plants. PRX4 is induced in vitro conditions since it was observed only in callus tissue and ceil suspensions,This study detected high uniformity and genetic stability within the regenerated plants in 100% of the sugarcane cultivars used.

scholarly journals Time of In Vitro Anther Culture May Moderate Action of Copper and Silver Ions that Affect the Relationship between DNA Methylation Change and the Yield of Barley Green Regenerants

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1064
Author(s):  
Piotr T. Bednarek ◽  
Renata Orłowska
Keyword(s):  
Anther Culture ◽  
De Novo ◽  
Silver Ions ◽  
Dna Demethylation ◽  
Induction Medium ◽  
Green Plants ◽  
Ion Concentrations ◽  
Regenerated Plants ◽  
Short Time

Plant anther culture allows for the regeneration of uniform and homozygous double haploids. However, off-type regenerants may appear as a result of so-called tissue culture-induced variation (TCIV). In addition, the presence of Cu2+ and Ag+ ions in the culture medium might influence the number of green plants. The regenerants were obtained via anther cultures of barley under varying Cu2+ and Ag+ ion concentrations in the induction medium during distinct time conditions. DArTseqMet markers were evaluated based on regenerants and donor plants and delivering data on DNA demethylation (DM) and de novo methylation (DNM) and changes in methylation (Delta). The number of green regenerated plants per 100 anthers (GPs) was evaluated. The Cu2+ and Ag+ ion concentrations moderated relationships between Delta and the number of green plants conditional on time of tissue cultures. Depending on the ions, moderated moderation is valid within the different time of anther culture. When the highest concentration of copper is analyzed, plant regeneration is possible under short ‘Time’ (21 days) of anther culture wherein Delta is negative or under elongated Time when Delta is positive. Under 21 days of culture, the highest concentration of silver ions and when Delta is negative, some regenerants could be evaluated. However, under high Ag+ concentration when Time of culture is long and Delta positive, the highest number of green plants could be obtained.

scholarly journals In vitro propagation of Boerhaavia diffusa L.: An important medicinal plant of family Nyctagimaceae

2019 ◽  
Vol 79 (01) ◽  
Author(s):  
Ashu Pandey ◽  
Oshin Verma ◽  
Suresh Chand
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
Liquid Medium ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Half Strength ◽  
Boerhaavia Diffusa ◽  
Important Medicinal Plant

Boerhaavia diffusa L., also known as santhi, or punarnava is an important medicinal plant, belonging to the family Nyctaginaceae. This species is said to be distributed throughout Malwa plateau in central India, as per ayurvedic literature, but due to extensive commercial exploitation, the species has become vulnerable. For callus induction, leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4- dichlorophenoxy acetic acid (2, 4-D 2.26 µM-9.04 µM) and N 6-benzyladenine (BA 1.11 µM-4.44 µM), either alone or in combinations. Calli formed within 10-12 days of culture, followed by shoots regeneration within 20-25 days. Direct organogenesis was achieved from nodal explants in MS media fortified with 2,4-D (2.26 µM-9.04 µM) along with BA (1.11 µM-4.44 µM) within 20 days. Multiple shooting was observed during subculture of in vitro regenerated shoots when 2,4-D was replaced with α-naphthalene acetic acid (NAA). Rooting was achieved in MS medium fortified with 2.85 µM IAA, within 7-10 days and also on half strength MS medium containing 2.85 µM Indole-3-acetic acid (IAA). For hardening, regenerated plants, with roots (3-4cm) were initially maintained on half-strength MS liquid medium (MS1/2) without growth regulators followed by quarter strength MS (MS1/4) liquid medium for 10 days. For acclimatization sterile mixture of soil, sand and manure (2:1:1) was used. Survival rate of regenerated plants was nearly 100%.

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