Callus culture and micropropagation of Eustoma grandiflorum Shinn.

Regenerated plants and callus tissue were obtained by embryo culture and micropropagation of explants from shoots and leaves of <em>Eustoma grandiflorum</em> Shinn. (<em>Gentianaceae</em>), a medicinal plant. This species, which does not naturally occur in this country, but is found in the southwestern regions of North America, can be propagated in vitro in a relatively short time. Regenerated plants were obtained in amounts enabling extensive phytochemical analyses to be conducted.