scholarly journals Production of Procyanidin compound in seeds , callus and in regenerated plants from vitis vinifera L.

2010 ◽  
Vol 4 (2) ◽  
pp. 76-83
Author(s):  
Karem T. K. AL-Hatemy
Keyword(s):  
Vitis Vinifera ◽  
High Performance ◽  
Callus Tissue ◽  
Mother Plant ◽  
Vitis Vinifera L ◽  
Ms Medium ◽  
Grape Seeds ◽  
Extraction And Purification ◽  
Regenerated Plants ◽  
Regenerated Plant

This experiment was conducted in Science and Agriculture College labs, Kufa University, on 2008 to describe methods for extraction and purification of Procyanidin compound from each of grape seeds mother plant(Vitis vinifera L.) ,callus induced from Axillary buds by using MS medium supplemented with 2,4-D at concentration of (1,2,3)mg/l and from leaves of regenerated from callus by using MS medium supplemented with ( IAA )at concentration of 2mg/l and (BA) benzyl adenine at concentration of 0.2 mg/l and from cocoas powdered (control) as well these Procyanidin. The content of Procyanidin were done by using HPLC (high performance liquid chromatography) and compare of the quantitatively of Procyanidin with these seeds in mother plant and callus tissue. This study include alcohol extraction by using mixture of ethanol, methanol, D.W and HCL. The result revealed that, the superiority of Procyanidin in seeds content than the content in callus and regenerated plant and increase the content of these compound in the leaves of regenerated plant with the content in callus tissue.

scholarly journals Effects of post-veraison irrigation on the phenolic composition of Vitis vinifera L. cv. ‘Xinomavro’ grapes

OENO One ◽  
2021 ◽  
Vol 55 (3) ◽  
pp. 173-189
Author(s):  
Seraphim Theocharis ◽  
Nikolaos Nikolaou ◽  
Eleftheria Zioziou ◽  
Maria Kyraleou ◽  
Stamatina Kallithraka ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
High Performance ◽  
Effective Means ◽  
High Performance Liquid Chromatographic ◽  
Vitis Vinifera L ◽  
Continuous Irrigation ◽  
Ultraviolet Absorbance ◽  
Absorbance Detection ◽  
Tandem Mass Spectrometric ◽  
Liquid Chromatographic

‘Xinomavro’ (the second planted red Greek variety behind ‘Agiorgitiko’) generally produces wines that are light in colour but with increased astringency, possibly related to grape flavonoid amount and composition; although irrigation is accepted as an effective means to enhance grape phenolic maturity, its role has not yet been sufficiently studied in the case of Xinomavro. This study aimed to determine the effect of post-veraison irrigation on berry anthocyanin and proanthocyanidin amount and composition, of field-grown Xinomavro vines (Vitis vinifera L.), under the typical summer conditions of Northern Greece. In a 10-year-old Xinomavro vineyard, two post-veraison watering regimes were applied—irrigation starting 20 days after veraison (mid-ripening irrigation, MRI) and irrigation starting immediately after veraison was completed (after veraison irrigation, AVI)—alongside non irrigated vines (NI), and vines irrigated continuously from berry set through harvest (continuous irrigation, CI). Proanthocyanidin composition was determined in both skins and seeds by employing phloroglucinolysis followed by HPLC-UV and MS detection (high-performance liquid chromatographic with tandem mass spectrometric and ultraviolet absorbance detection), and the anthocyanin profile was identified only in the skin extracts by HPLC-UV (high-performance liquid chromatographic with ultraviolet absorbance detection). Post-veraison irrigation increased yield parameters and reduced anthocyanin levels and the proportion of their stable forms (acylated vs. non-acylated, tri-oxygenated and methoxylated on the B-ring vs. di-oxygenated and hydroxylated), compared to NI vines; however, these effects were more pronounced in the case of early post-veraison irrigation (AVI) than late-season irrigation (MRI). Irrigation also increased the mean Degree of Polymerization (mDP) and prodelphinidin percentage of skin tannins and decreased mDP of seed tannins. In the light of the necessity to control the accelerated ripening under the increasingly hotter and drier climatic conditions, late irrigation (MRI) might provide a solution to avoid excessive sugar levels and allow a slightly higher yield without significant reductions in berry phenolic content. The results suggest that the optimisation of the timing of irrigation could provide an effective adaptation strategy to climate change in Mediterranean viticultural areas.

scholarly journals Flavanol Glycoside Content of Grape Seeds and Skins of Vitis vinifera Varieties Grown in Castilla-La Mancha, Spain

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 4001 ◽  
Author(s):  
José Pérez-Navarro ◽  
Guillaume Cazals ◽  
Christine Enjalbal ◽  
Pedro Miguel Izquierdo-Cañas ◽  
Sergio Gómez-Alonso ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
High Performance ◽  
Grape Seed ◽  
Grape Seeds ◽  
Grape Berries ◽  
La Mancha ◽  
Liquid Chromatography Tandem Mass ◽  
Grape Varieties ◽  
Grape Seed Extracts ◽  
Seed Extracts

Glycosylated flavanols (monoglycosides and diglycosides) in skin and seed extracts of Vitis vinifera grapes grown in Castilla-La Mancha (Spain) were investigated using ultra-high-performance liquid chromatography—tandem mass spectrometry (UHPLC-ESI-QQQ-MS/MS). Six grape varieties (Airén, Tempranillo, the recently identified Albillo Dorado, Montonera del Casar, Moribel, and Tinto Fragoso) were studied over two consecutive years (2016 and 2017). A total of twenty monomeric flavanol monoglycosides, four diglycosylated monomers, and three dimeric flavanol monoglycosides were detected in all grape samples. The diversity observed in the composition of glycosylated flavanol in the grape berries suggests a strong influence of variety and grape tissue (skin or seed). Monomeric flavanol glycosides were more abundant in grape seed extracts, in contrast with monoglycosylated dimeric forms. In addition, the glycosylated flavanol content was related to berry color in grape skins, with higher concentrations measured in black grape varieties.

Phenolic composition and physicochemical parameters of Carménère, Cabernet Sauvignon, Merlot and Cabernet Franc grape seeds (Vitis vinifera L.) during ripening

LWT ◽  
2012 ◽  
Vol 48 (1) ◽  
pp. 134-141 ◽  
Author(s):  
Elías Obreque-Slier ◽  
Remigio López-Solís ◽  
Lorena Castro-Ulloa ◽  
Cristian Romero-Díaz ◽  
Álvaro Peña-Neira
Keyword(s):  
Vitis Vinifera ◽  
Physicochemical Parameters ◽  
Vitis Vinifera L ◽  
Cabernet Sauvignon ◽  
Phenolic Composition ◽  
Grape Seeds ◽  
Cabernet Franc

scholarly journals Grape (Vitis vinifera L.) Seed Oil: A Functional Food from the Winemaking Industry

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1360
Author(s):  
Maria E Martin ◽  
Elena Grao-Cruces ◽  
Maria C Millan-Linares ◽  
Sergio Montserrat-de la Paz
Keyword(s):  
Vitis Vinifera ◽  
Seed Oil ◽  
Grape Seed ◽  
Vitis Vinifera L ◽  
Grape Seeds ◽  
Wine Production ◽  
Food Industries ◽  
Grape Seed Oil ◽  
Antioxidant Effects

Wine production is an ancient human activity that generates several by-products, which include some constituents known for their potential in health care and for their role in the food or cosmetic industries. Any variety of grape (Vitis vinifera L.) contains nutrients and bioactive compounds available from their juice or solid parts. Grape seed extract has demonstrated many activities in disease prevention, such as antioxidant effects, which make it a potential source of nutraceuticals. Grape seed is a remarkable winery industry by-product due to the bioactivity of its constituents. Methods for recovery of oil from grape seeds have evolved to improve both the quantity and quality of the yield. Both the lipophilic and hydrophilic chemicals present in the oil of V. vinifera L. make this wine by-product a source of natural nutraceuticals. Food and non-food industries are becoming novel targets of oil obtained from grape seeds given its various properties. This review focuses on the advantages of grape seed oil intake in our diet regarding its chemical composition in industries not related to wine production and the economic and environmental impact of oil production.

Inhibitory effect of phenolic compounds from grape seeds (Vitis vinifera L.) on the activity of angiotensin I converting enzyme

LWT ◽  
2013 ◽  
Vol 54 (1) ◽  
pp. 265-270 ◽  
Author(s):  
Joana Afonso ◽  
Cláudia P. Passos ◽  
Manuel A. Coimbra ◽  
Carlos M. Silva ◽  
Patrício Soares-da-Silva
Keyword(s):  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
Inhibitory Effect ◽  
Vitis Vinifera L ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Grape Seeds ◽  
Angiotensin I Converting Enzyme

scholarly journals BIOCHEMICAL AND PHYSIOLOGICAL CHANGES OF CALLUS GROWTH AND LYCOPENE PIGMENT PRODUCTION FROM TOMATO (Lycopersicon esculentum Mill.) UNDER DROUGHT STRESS

2018 ◽  
pp. 7-21
Author(s):  
Talib Khashan Kareem ◽  
Abbas Tikki Karrar
Keyword(s):  
Drought Stress ◽  
Lycopersicon Esculentum ◽  
Callus Tissue ◽  
Tomato Fruit ◽  
Sugar Content ◽  
Mother Plant ◽  
Ms Medium ◽  
Lycopersicon Esculentum Mill

This experiment was conducted in faculty of Science labs, Kufa University, carried out during 2015 to applied methods for extraction, purification and Quantitative of Lycopene red pigments, from callus tissue and tomato fruits mother plant (Lycopersicon esculentum Mill).This study include of three parts, Firstly; Tomato seeds(Supper queen) hybrid were germinated in free MS medium and callus induction from shoot tip (3cmpieces) by using MS medium supplemented with Dichlorophenoxiactic acid (2,4-D) at different concentration (0.5,1, 1.5mg/l)with benzyl adenine (BA) at concentration of (0.3 mg/l). Secondly; identically callus fresh weight re-cultured in the same MS medium supplemented with high molecular weight polyethylene glycol (PEG) was used as selective agent at level of (5,10,15 and 25%). Thirdly; comparisons study were made between in vitro and in vivo grown plant. Powder of control lycopene used as standard solution. The content of lycopene was done by using high performance liquid chromatography (HPLC), and compare of the quantitatively of lycopene with these content in fruits of mother plant, and callus tissue. Also, include alcohol extraction of Lycopene from tomato fruit by using acetone and hexane mixture. The result showed significant increased (P< 0.05) of lycopene production and the superiority of lycopene content in callus than the content in fruits of mother plant. Antioxidant enzymes activity like Catalase (CAT),Guaiacol peroxidise (POX) and Superoxide dismutase(SOD) were high in callus under drought stress than in fruit of mother plant. However, Proline and total sugar content were at higher levels in callus under drought stress than in fruit of mother plant.

scholarly journals The Study of Antioxidant Components in Grape Seeds

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3736 ◽  
Author(s):  
Lenka Sochorova ◽  
Bozena Prusova ◽  
Tunde Jurikova ◽  
Jiri Mlcek ◽  
Anna Adamkova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitis Vinifera ◽  
Ferulic Acid ◽  
Gallic Acid ◽  
Antioxidant Properties ◽  
Polyphenolic Compounds ◽  
Vitis Vinifera L ◽  
Coumaric Acid ◽  
Grape Seeds ◽  
Chromatographic Techniques

The paper deals with the study of antioxidant properties of extracts from vine seeds (Vitis vinifera L.) using spectrometric and chromatographic techniques. Ten vine varieties (Cerason, Laurot, Kofranka, Gewürztraminer, Hibernal, Blaufrankisch, Zweigeltrebe, Erilon, Palava, and Welschriesling) obtained from the years 2015, 2016, and 2017 were selected for the study. The antioxidant activity was determined spectrophotometrically using four fundamentally different methods; the content of total polyphenolic compounds was determined using the Folin–Ciocalteu method. In 2015, the content of 14 antioxidants (gallic acid, caffeic acid, coumaric acid, coutaric acid, ferulic acid, fertaric acid, trans-piceid, trans-piceatannol, rutin, quercetin-3-β-d-glucoside, quercitrin, myricetin, catechin, and epicatechin) were studied. The results of the study show the high content of antioxidant components in grape seeds and the differences in content in individual varieties and in individual years.

scholarly journals Identification and Quantification of Flavanol Glycosides in Vitis vinifera Grape Seeds and Skins during Ripening

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2745 ◽  
Author(s):  
Marie Zerbib ◽  
Guillaume Cazals ◽  
Christine Enjalbal ◽  
Cédric Saucier
Keyword(s):  
Vitis Vinifera ◽  
High Performance ◽  
Multiple Reaction Monitoring ◽  
Reaction Monitoring ◽  
Quadrupole Mass ◽  
Grape Seeds ◽  
Grape Berries ◽  
Grape Skins ◽  
Grape Varieties ◽  
First Time

Monomeric and dimeric flavanol glycosides were analyzed in Vitis vinifera grapes and seeds during ripening. An analytical method using ultra-high performance liquid chromatography coupled with a triple quadrupole mass spectrometry (UHPLC-ESI-QQQ-MS/MS) in multiple reaction monitoring (MRM) mode was employed. Three grape varieties (Merlot, Syrah and Tannat) were chosen and grape berries were sampled at different stages of development. Ten monoglycosylated and six diglycosylated flavanol monomers were detected. Twelve monoglycosylated and three diglycosylated flavanol dimers were also detected for all three grape varieties. All diglycosides were detected for the first time in Vitis vinifera grapes, though some of these compounds were only detected in skins or seeds. Furthermore, the evolution of all these compounds was studied, and a decrease in monomeric (epi) catechin monoglycosides was observed during ripening for Tannat, Merlot and Syrah grape skins. The dimers would appear to accumulate in skin tissues up to mid-summer (after veraison) and decrease when grape berries reached maturity.

scholarly journals Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

2012 ◽  
Vol 17 (3) ◽  
Author(s):  
Ewa Olchowik ◽  
Karol Lotkowski ◽  
Saidmukhtar Mavlyanov ◽  
Nodira Abdullajanova ◽  
Maksim Ionov ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Condensed Tannins ◽  
Antioxidant Activities ◽  
Erythrocyte Membranes ◽  
Vitis Vinifera L ◽  
Grape Seeds ◽  
High Concentration ◽  
Hypotonic Stress ◽  
Hydrolysable Tannins ◽  
Rhus Typhina

AbstractErythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activities of polyphenol-rich plant extracts containing hydrolysable tannins from sumac leaves (Rhus typhina L.) and condensed tannins from grape seeds (Vitis vinifera L.) showed that at the 5–50 μg/ml concentration range they reduced to the same extent hemolysis and glutathione, lipid and hemoglobin oxidation induced by erythrocyte treatment with 400 μM ONOO− or 1 mM HClO. However, extract (condensed tannins) from grape seeds in comparison with extract (hydrolysable tannins) from sumac leaves stabilized erythrocytes in hypotonic NaCl solutions weakly. Our data indicate that both hydrolysable and condensed tannins significantly decrease the fluidity of the surface of erythrocyte membranes but the effect of hydrolysable ones was more profound. In conclusion, our results indicate that extracts from sumac leaves (hydrolysable tannins) and grape seeds (condensed tannins) are very effective protectors against oxidative damage in erythrocytes.

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