Inhibitory effects of tunicamycin on cell-cell adhesion and cell reaggregation of the cellular slime mold,Polysphondylium pallidum

1996 ◽  
Vol 109 (2) ◽  
pp. 193-199 ◽  
Author(s):  
Nobuyuki Nakata ◽  
Hiroshi Ochiai
Keyword(s):  
Cell Adhesion ◽  
Slime Mold ◽  
Cellular Slime Mold ◽  
Inhibitory Effects ◽  
Polysphondylium Pallidum ◽  
Cellular Slime ◽  
Cell Cell

scholarly journals Identification and purification of an endogenous receptor for the lectin pallidin from Polysphondylium pallidum.

1982 ◽  
Vol 93 (2) ◽  
pp. 383-389 ◽  
Author(s):  
D K Drake ◽  
S D Rosen
Keyword(s):  
Cell Adhesion ◽  
Cell Surface ◽  
Marine Sponges ◽  
Cellular Slime Mold ◽  
Extracellular Medium ◽  
Polysphondylium Pallidum ◽  
Food Substance ◽  
Apparent Homogeneity ◽  
Cellular Slime ◽  
Cell Cell

We report the identification and purification of an endogenous carbohydrate-containing receptor of pallidin, the cell surface lectin implicated in mediating cell-cell adhesion in the cellular slime mold Polysphondylium pallidum. The receptor is identified in an aqueous extract of crude P. pallidum membranes as a potent inhibitor of the hemagglutination activity of pallidin. The inhibitor is purified to apparent homogeneity by affinity precipitation with pallidin followed by fractionation of the solubilized precipitate on Sepharose 4B. The hemagglutination inhibitor (HAI) is metabolically radiolabeled, indicating that it is a biosynthetic product of the amoebae and not an ingested food substance. The HAI is released into the extracellular medium by living, differentiated amoebae. This release is markedly facilitated by the addition of D-galactose, a specific saccharide that binds to pallidin. Hence, the HAI appears to have an in situ association with pallidin at the cell surface. Exogenously added HAI promotes the agglutination of differentiated amoebae in a gyrated suspension at very low concentrations. The results are consistent with a model of cell-cell adhesion in which the HAI is a multivalent, extracellular aggregation factor that is recognized by pallidin molecules on adjacent cells. The HAI would then be analogues to the aggregation factors identified in marine sponges.

Antisense RNA inactivation of gene expression of a cell-cell adhesion protein (gp64) in the cellular slime mold Polysphondylium pallidum

1996 ◽  
Vol 109 (5) ◽  
pp. 1009-1016
Author(s):  
S. Funamoto ◽  
H. Ochiai
Keyword(s):  
Gene Expression ◽  
Cell Adhesion ◽  
Antisense Rna ◽  
Resistant Cell ◽  
Cell Contact ◽  
Cellular Slime Mold ◽  
Adhesion Protein ◽  
Cell Adhesion Protein ◽  
Polysphondylium Pallidum ◽  
Cell Cell

The gp64 protein of Polysphondylium pallidum has been shown to mediate EDTA-stable cell-cell adhesion. To explore the functional role of gp64, we made an antisense RNA expression construct designed to prevent the gene expression of gp64; the construct was introduced into P. pallidum cells and the transformants were characterised. The antisense RNA-expressing clone L3mc2 which had just been harvested at the growth phase tended to re-form in aggregates smaller in size than did the parental cells in either the presence or absence of 10 mM EDTA. In contrast, 6.5-hour starved L3mc2 cells remained considerably dissociated from each other after 5 minutes gyrating, although aggregation gradually increased by 50% during a further 55 minutes gyrating in the presence of 10 mM EDTA. Correspondingly, L3mc2 lacked specifically the cell-cell adhesion protein, gp64. We therefore conclude that the gp64 protein is involved in forming the EDTA-resistant cell-cell contact. In spite of the absence of gp64, L3mc2 exhibited normal developmental processes, a fact which demonstrates that another cell-cell adhesion system exists in the development of Polysphondylium. This is the first report in which an antisense RNA technique was successfully applied to Polysphondylium.

Induction of encystment of Polysphondylium pallidum amoeba

1977 ◽  
Vol 23 (5) ◽  
pp. 518-521 ◽  
Author(s):  
Joseph Lonski ◽  
Nicholas Pesut
Keyword(s):  
Slime Mold ◽  
Cellular Slime Mold ◽  
Polysphondylium Pallidum ◽  
Cellular Slime

The induction of microcyst formation could be triggered in washed amoebae of the cellular slime mold Polysphondylium pallidum (strain-2) by the addition of 2 mM ethionine. Methionine at a ratio of 2:1 with ethionine would inhibit microcyst induction by ethionine. The involvement of polyamines in morphogenesis was also shown. Putrescine (0.02 to 0.1 M) induced the formation of microcysts, whereas spermidine (2 to 4 mM) was capable of causing a fourfold reduction in 0.05 M putrescine-induced microcysts but incapable of inhibiting microcyst induction by 0.08 M putrescine. Glycerol (0.5 M or 0.4 M) was also found to be an effective inducer of microcysts.

scholarly journals Spatial patterns in the fruiting bodies of the cellular slime mold Polysphondylium pallidum

1988 ◽  
Vol 38 (2) ◽  
pp. 73-81 ◽  
Author(s):  
Edward C. Cox ◽  
Fred W. Spiegel ◽  
Gerard Byrne ◽  
James W. McNally ◽  
Leslie Eisenbud
Keyword(s):  
Spatial Patterns ◽  
Slime Mold ◽  
Cellular Slime Mold ◽  
Fruiting Bodies ◽  
Polysphondylium Pallidum ◽  
Cellular Slime

Synthesis of macromolecules during microcyst germination in the cellular slime mold Polysphondylium pallidum

1978 ◽  
Vol 65 (2) ◽  
pp. 251-259 ◽  
Author(s):  
Herbert L. Ennis ◽  
Diane Pennica ◽  
Janet M. Hill
Keyword(s):  
Slime Mold ◽  
Cellular Slime Mold ◽  
Polysphondylium Pallidum ◽  
Cellular Slime
Sign in / Sign up

Export Citation Format

Share Document