rtpA, a gene encoding a bacterial two-component sensor kinase, determines pathogenic traits of Pseudomonas tolaasii, the causal agent of brown blotch disease of a cultivated mushroom, Pleurotus ostreatus

Mycoscience ◽  
1998 ◽  
Vol 39 (3) ◽  
pp. 261-271 ◽  
Author(s):  
Hitoshi Murata ◽  
Takanori Tsukamoto ◽  
Akira Shirata
Keyword(s):  
Pleurotus Ostreatus ◽  
Causal Agent ◽  
Sensor Kinase ◽  
Gene Encoding ◽  
Pseudomonas Tolaasii ◽  
Two Component ◽  
Cultivated Mushroom ◽  
Brown Blotch ◽  
Brown Blotch Disease

scholarly journals Pseudomonas costantinii sp. nov., another causal agent of brown blotch disease, isolated from cultivated mushroom sporophores in Finland.

2002 ◽  
Vol 52 (6) ◽  
pp. 1973-1983 ◽  
Author(s):  
Patricia Munsch ◽  
Tapani Alatossava ◽  
Nina Marttinen ◽  
Jean-Marie Meyer ◽  
Richard Christen ◽  
...  
Keyword(s):  
Causal Agent ◽  
Cultivated Mushroom ◽  
Brown Blotch ◽  
Brown Blotch Disease

scholarly journals The Erwinia chrysanthemi 3937 PhoQ Sensor Kinase Regulates Several Virulence Determinants

2006 ◽  
Vol 188 (8) ◽  
pp. 3088-3098 ◽  
Author(s):  
Balakrishnan Venkatesh ◽  
Lavanya Babujee ◽  
Hui Liu ◽  
Pete Hedley ◽  
Takashi Fujikawa ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Regulatory System ◽  
Soft Rot ◽  
Erwinia Chrysanthemi ◽  
Pcr Analysis ◽  
Sensor Kinase ◽  
Virulence Determinants ◽  
Gene Encoding ◽  
Reverse Transcription Pcr ◽  
Two Component

ABSTRACT The PhoPQ two-component system regulates virulence factors in Erwinia chrysanthemi, a pectinolytic enterobacterium that causes soft rot in several plant species. We characterized the effect of a mutation in phoQ, the gene encoding the sensor kinase PhoQ of the PhoPQ two-component regulatory system, on the global transcriptional profile of E. chrysanthemi using cDNA microarrays and further confirmed our results by quantitative reverse transcription-PCR analysis. Our results indicate that a mutation in phoQ affects transcription of at least 40 genes, even in the absence of inducing conditions. Enhanced expression of several genes involved in iron metabolism was observed in the mutant, including that of the acs operon that is involved in achromobactin biosynthesis and transport. This siderophore is required for full virulence of E. chrysanthemi, and its expression is governed by the global repressor protein Fur. Changes in gene expression were also observed for membrane transporters, stress-related genes, toxins, and transcriptional regulators. Our results indicate that the PhoPQ system governs the expression of several additional virulence factors and may also be involved in interactions with other regulatory systems.

Characterization of bacteriophage ϕPto-bp6g, a novel phage that lyses Pseudomonas tolaasii causing brown blotch disease in mushrooms

2012 ◽  
Vol 91 (3) ◽  
pp. 514-519 ◽  
Author(s):  
Hanh Thi Dieu Nguyen ◽  
Soojin Yoon ◽  
Min-Hee Kim ◽  
Young-Kee Kim ◽  
Moon-Young Yoon ◽  
...  
Keyword(s):  
Pseudomonas Tolaasii ◽  
Brown Blotch ◽  
Brown Blotch Disease

scholarly journals Resistance Sources to Brown Blotch Disease (Pseudomonas tolaasii) in a Diverse Collection of Pleurotus Mushroom Strains

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 227 ◽  
Author(s):  
Benjamin Azu Okorley ◽  
Frederick Leo Sossah ◽  
Dan Dai ◽  
Shuai Xu ◽  
Zhenghui Liu ◽  
...  
Keyword(s):  
Pseudomonas Tolaasii ◽  
Resistance Sources ◽  
Controlled Conditions ◽  
Resistant Strains ◽  
Brown Blotch ◽  
Brown Blotch Disease ◽  
Moderately Resistant

Brown blotch disease (BBD) caused by Pseudomonas tolaasii is one of the most devastating diseases of Pleurotus spp. worldwide. Breeding for resistant strains is the most effective method for controlling BBD. To identify resistant germplasm for BBD management, 97 strains comprising 21 P. cf. floridanus, 20 P. ostreatus, and 56 P. pulmonarius were screened by two different methods; namely, inoculation of the pathogen on the mushroom pileus (IMP) and on the spawned substrate (IMSS) under controlled conditions. Out of the 97 strains screened, 22 P. pulmonarius, and four P. cf. floridanus were moderately resistant to BBD using the IMP method. Eleven P. pulmonarius, six P. cf. florida, and one P. ostreatus strains were highly resistant to BBD using the IMSS method. All of the 97 strains showed varying degrees of susceptibility using the IMP method, but eight strains were completely resistant using the IMSS method. Combining these two methods, five strains were highly resistant (four P. pulmonarius and one P. cf. floridanus) and 11 were moderately resistant (eight P. pulmonarius and three P. cf. floridanus). The resistance sources to P. tolaasii identified in P. pulmonarius and P. cf. floridanus could be used for further breeding of Pleurotus spp.

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