Proliferation of epithelial cells derived from rat dorsolateral prostate in serum-free primary cell culture and their response to androgen

1988 ◽  
Vol 24 (8) ◽  
pp. 778-786 ◽  
Author(s):  
Nozomu Nishi ◽  
Yuhsi Matuo ◽  
Takahisa Nakamoto ◽  
Fumio Wada
Keyword(s):  
Cell Culture ◽  
Epithelial Cells ◽  
Primary Cell Culture ◽  
Primary Cell ◽  
Serum Free

scholarly journals A method for the isolation of human gastric mucous epithelial cells for primary cell culture: A comparison of biopsy vs surgical tissue

1996 ◽  
Vol 18 (4) ◽  
pp. 269-281 ◽  
Author(s):  
Michael J. Rutten ◽  
Donald R. Campbell ◽  
Cheryl A. Luttropp ◽  
Wendy M. Fowler ◽  
Mitchell A. Hawkey ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cells ◽  
Primary Cell Culture ◽  
Primary Cell ◽  
Gastric Mucous

scholarly journals Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model

2020 ◽  
Vol 2020 ◽  
pp. 1-15 ◽  
Author(s):  
Katarzyna Wojtanowicz-Markiewicz ◽  
Magdalena Kulus ◽  
Sandra Knap ◽  
Ievgenia Kocherova ◽  
Maurycy Jankowski ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cells ◽  
Primary Cell Culture ◽  
Water Movement ◽  
Inorganic Ions ◽  
Primary Cell ◽  
Lag Phase ◽  
Luminal Epithelial Cells

Luminal epithelial cells are the first embryonic-maternal contact site undergoing very specific changes associated with reproductive processes. Cells prepare for embryo development by increasing their volume, with the help of aquaporins that provide a transcellular path of rapid water movement during the secretion and absorption of fluids, as well as connexins enabling the flow of inorganic ions and small molecules. In this work, we have examined how AQPs and Cx’s behave in luminal epithelium primary cell culture. Cells obtained from porcine specimen during slaughter were primarily in vitro cultured for 7 days. Their proliferation patterns were then analyzed using RTCA, with the expression of genes of interest evaluated with the use of immunofluorescence and RT-qPCR. The results of these changes of gene of interest expression were analyzed on each of the seven days of the porcine luminal primary cell culture. Our study showed that the significant changes were noted in the case of Cx43, whose level of protein expression and distribution increases after 120 hours of culture, when the cells enter the lag phase, and maintains an upward trend until the end of the culture. We noted an increase in AQP4, AQP7, AQP8, and AQP11 levels throughout the entire culture period, while the largest differences in expression were found in AQP3, AQP4, and AQP10. The obtained results could become a point of reference for further in vivo and clinical research. Experiments conducted with these proteins showed that they influence the endometrial fluid content during the oestrous cycle and participate in the process of angiogenesis, which intensifies during endometrial development.

Effects of actinomycin D and cycloheximide on the increase in tyrosinase activity of hamster amelanotic

1984 ◽  
Vol 4 (12) ◽  
pp. 1059-1064 ◽  
Author(s):  
A. Słomiñski ◽  
A. Bomirski ◽  
P. W. D. Ścisłowski ◽  
S. Żołnierowicz
Keyword(s):  
Cell Culture ◽  
Primary Cell Culture ◽  
Actinomycin D ◽  
Calf Serum ◽  
Amelanotic Melanoma ◽  
Tyrosinase Activity ◽  
Primary Cell ◽  
Serum Free ◽  
Activation Of Transcription

Tyrosinase activity in the Ab hamster amelanotic melanoma ceils cultured in serum-free Eagle's MEM increased 3 times after 6 h of primary cell culture. This increase was inhibited completely by cycloheximide, while actinomycin D had no effect on this process. After 24 h of culture in MEM with calf serum, further increase of the tyrosinase activity was inhibited by both cycloheximide and actinomycin D. The data presented may indicate that the increase of tyrosinase activity in the primary cell culture of the Ab melanoma is due initially to the unblocking of translation and later to the activation of transcription of the gene controlling the enzyme.

Measuring clinically relevant endpoints in a serum-free, three-dimensional, primary cell culture system of human osteoarthritic articular chondrocytes

2017 ◽  
Vol 357 (2) ◽  
pp. 310-319
Author(s):  
Grace Bundens ◽  
Andrea Buckley ◽  
LaBraya Milton ◽  
Kathryn Behling ◽  
Sarah Chmielewski ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Primary Cell Culture ◽  
Articular Chondrocytes ◽  
Three Dimensional ◽  
Primary Cell ◽  
Cell Culture System ◽  
Serum Free

Primary Cell Culture of Turkey Intestinal Epithelial Cells

1996 ◽  
Vol 40 (1) ◽  
pp. 103 ◽  
Author(s):  
Akbar Ali ◽  
Donald L. Reynolds
Keyword(s):  
Cell Culture ◽  
Epithelial Cells ◽  
Primary Cell Culture ◽  
Intestinal Epithelial Cells ◽  
Primary Cell ◽  
Intestinal Epithelial

The role of ER stress for the pathogenesis of SCA3 in primary cell culture experiment

2007 ◽  
Vol 34 (S 2) ◽  
Author(s):  
C Funke ◽  
J Hübener ◽  
H Wolburg ◽  
T Schmidt ◽  
H Toresson ◽  
...  
Keyword(s):  
Cell Culture ◽  
Er Stress ◽  
Primary Cell Culture ◽  
Primary Cell ◽  
Culture Experiment ◽  
Cell Culture Experiment
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