Human oral epithelial cell culture I. Improved conditions for reproducible culture in serum-free medium

1990 ◽  
Vol 26 (6) ◽  
pp. 589-595 ◽  
Author(s):  
Dolphine Oda ◽  
Eileen Watson
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Serum Free Medium ◽  
Free Medium ◽  
Epithelial Cell Culture ◽  
Serum Free ◽  
Oral Epithelial Cell ◽  
Oral Epithelial ◽  
Human Oral Epithelial Cell

Evaluation of Buccal Methyl-β-Cyclodextrin Toxicity on Human Oral Epithelial Cell Culture Model

2005 ◽  
Vol 94 (6) ◽  
pp. 1300-1309 ◽  
Author(s):  
Laïla Boulmedarat ◽  
Amélie Bochot ◽  
Sylviane Lesieur ◽  
Elias Fattal
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Cell Culture Model ◽  
Epithelial Cell Culture ◽  
Oral Epithelial Cell ◽  
Culture Model ◽  
Oral Epithelial ◽  
Human Oral Epithelial Cell

Islet Cell Culture in Defined Serum-Free Medium*

Endocrinology ◽  
1990 ◽  
Vol 126 (4) ◽  
pp. 1895-1903 ◽  
Author(s):  
SAMUEL A. CLARK ◽  
WILLIAM L. CHICK
Keyword(s):  
Cell Culture ◽  
Islet Cell ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Islet Cell Culture

Effects of vitamin A on growth and differentiation of human tracheobronchial epithelial cell cultures in serum-free medium

1989 ◽  
Vol 93 (1) ◽  
pp. 133-142
Author(s):  
D.P. Chopra ◽  
M.M. Klinger ◽  
J.K. Sullivan
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Vitamin A ◽  
Cell Cultures ◽  
Cell Multiplication ◽  
Serum Free Medium ◽  
Free Medium ◽  
Plasma Membrane Vesicles ◽  
Serum Free ◽  
Epithelial Cell Cultures

Differentiating epithelial cell cultures from human tracheobronchial epithelium have been propagated in serum-free medium. The major objective of this study was to examine the trophic effects of vitamin A on cell multiplication and morphology of the tracheal cell cultures. The cellular responses were analyzed in terms of growth kinetics, morphological and ultrastructural alterations and secretion of glycoconjugates. Cell cultures in control medium exhibited characteristics of epithelial cells including microvilli on cell surfaces, desmosomes between cells, and numerous secretory vesicles in the cytoplasm. Vitamin A at 10(−6) M and 10(−7) M inhibited cell replication and enhanced the secretion of [3H]glucosamine-labeled glycoconjugates. Further, vitamin A increased the production of plasma membrane vesicles and acquisition by the cells of a highly secretory ultrastructure. This in vitro model of human epithelial cells will be important in the investigation of various aspects of growth and differentiation.

A DEFINED SERUM-FREE MEDIUM FOR DIVERSE CELL CULTURE APPLICATIONS

1992 ◽  
pp. 155-157
Author(s):  
A. Handa-Corrigan; ◽  
A. Garcia de Castro ◽  
M. Chadd ◽  
S. Zhang
Keyword(s):  
Cell Culture ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free
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