Transient induction of cytochrome P450 1A1 mRNA by culture medium component in primary cultures of adult rat hepatocytes

1993 ◽  
Vol 29 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Thomas A. Kocarek ◽  
Erin G. Schuetz ◽  
Philip S. Guzelian
Keyword(s):  
Cytochrome P450 ◽  
Culture Medium ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Cytochrome P450 1A1 ◽  
Adult Rat ◽  
Medium Component ◽  
Adult Rat Hepatocytes ◽  
Culture Medium Component

Induction and regulation of connexin26 by glucagon in primary cultures of adult rat hepatocytes

1995 ◽  
Vol 108 (8) ◽  
pp. 2771-2780 ◽  
Author(s):  
T. Kojima ◽  
T. Mitaka ◽  
Y. Shibata ◽  
Y. Mochizuki
Keyword(s):  
Culture Medium ◽  
Primary Cultures ◽  
Minor Component ◽  
Rat Hepatocytes ◽  
Primary Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Epidermal Growth ◽  
A Minor ◽  
Junction Proteins

In the adult rat hepatocyte, the gap junction proteins consist of a major component, connexin32 (Cx32) and a minor component, connexin26 (Cx26). Although we recently reported our success in inducing and maintaining Cx32 in adult rat hepatocytes cultured in serum-free L-15 medium supplemented with epidermal growth factor and 2% dimethyl sulfoxide, it was very difficult to induce Cx26 in the primary hepatocytes. In the present study, we found that the addition of 10(−7) M glucagon into the culture medium could dramatically induce Cx26 mRNA and protein. Although the expression of Cx32 mRNA was also influenced by glucagon, the increase of the expression was small. Immunocytochemically, Cx26-positive spots were observed between most adjacent cells and were co-localized with the Cx32-positive spots. We also examined whether 0.5 mM dibutyl cyclic AMP could induce expression of Cx26 in the cells. The effect of dexamethasone on the expression of Cx26 mRNA compared to that of Cx32 mRNA was examined. For the induction and maintenance of Cx26 mRNA, more than 10(−7) M dexamethasone was necessary in this culture. These results suggest that expression of Cx26 in hepatocytes may be regulated by the concentrations of glucagon and glucocorticoid hormones.

scholarly journals Regulation of ATP-citrate lyase in primary cultures of adult rat hepatocytes.

1979 ◽  
Vol 254 (23) ◽  
pp. 12169-12173
Author(s):  
J.T. Spence ◽  
H.C. Pitot ◽  
G. Zalitis
Keyword(s):  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Atp Citrate Lyase ◽  
Adult Rat ◽  
Citrate Lyase ◽  
Adult Rat Hepatocytes

Divergent effects of cycloheximide on the induction of Class II and Class III cytochrome P450 mRNAs in cultures of adult rat hepatocytes

1990 ◽  
Vol 281 (2) ◽  
pp. 204-211 ◽  
Author(s):  
Hans-Joerg Burger ◽  
Erin G. Schuetz ◽  
John D. Schuetz ◽  
Philip S. Guzelian
Keyword(s):  
Cytochrome P450 ◽  
Rat Hepatocytes ◽  
Class Ii ◽  
Class Iii ◽  
Adult Rat ◽  
Adult Rat Hepatocytes

Thymidine uptake in primary cultures of adult rat hepatocytes

1986 ◽  
Vol 14 (1) ◽  
pp. 101-102 ◽  
Author(s):  
ANNA ZAKHAROVA ◽  
HEATHER M. WALLACE
Keyword(s):  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Thymidine Uptake ◽  
Adult Rat ◽  
Adult Rat Hepatocytes
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