A simplified method for the detection of neomycin phosphotransferase II activity in transformed plant tissues

1987 ◽  
Vol 5 (4) ◽  
pp. 380-386 ◽  
Author(s):  
Raymond E. McDonnell ◽  
Robert D. Clark ◽  
Wendy A. Smith ◽  
Maud A. Hinchee
Keyword(s):  
Plant Tissues ◽  
Neomycin Phosphotransferase ◽  
Neomycin Phosphotransferase Ii ◽  
Simplified Method

A rapid immunoprecipitation assay for neomycin phosphotransferase II expression in transformed bacteria and plant tissues

1990 ◽  
Vol 68 (6) ◽  
pp. 983-987
Author(s):  
Chris L. Baszczynski
Keyword(s):  
Brassica Napus ◽  
Bovine Serum Albumin ◽  
Kanamycin Resistance ◽  
Plant Tissues ◽  
Nptii Gene ◽  
Neomycin Phosphotransferase ◽  
Kanamycin Resistance Gene ◽  
Dot Blot ◽  
Immunoprecipitation Assay ◽  
Neomycin Phosphotransferase Ii

Anti-kanamycin antibodies produced in rabbits, following coupling of the antibiotic to bovine serum albumin, were used to immunoprecipitate radioactively labelled phosphorylated kanamycin from transformed bacterial or plant extracts in a novel assay system, for the detection of neomycin phosphotransferase II (NPTII) activity. Radioactive counts in the immunoprecipitated pellet give a semiquantitative measure of the kanamycin phosphorylation and hence the amount of NPTII activity. This assay is sensitive, uses very small amounts of radioactivity, and is very rapid, allowing many samples to be processed within a few hours. Immunoprecipitated counts from reactions with bacteria carrying a kanamycin resistance gene or from tobacco and Brassica napus plants transformed with NPTII gene-containing vectors were consistently higher than counts from nontransformed controls. Results obtained with this assay correlate well with those from the previously described gel overlay and dot-blot assays, but can be obtained in an appreciably shorter time frame.Key words: anti-kanamycin antibodies, immunoprecipitation, neomycin phosphotransferase II assay, transformation.

scholarly journals Use of the gene encoding neomycin phosphotransferase II to convect linked markers into the vaccinia virus genome

1988 ◽  
Vol 16 (4) ◽  
pp. 1634-1634 ◽  
Author(s):  
Christine A. Franke ◽  
Dennis E. Hruby
Keyword(s):  
Vaccinia Virus ◽  
Virus Genome ◽  
Neomycin Phosphotransferase ◽  
Gene Encoding ◽  
Neomycin Phosphotransferase Ii

A simplified method for determining 1-aminocyclopropane-1-carboxylic acid (ACC) in plant tissues using a mass selective detector

1989 ◽  
Vol 8 (4) ◽  
pp. 297-307 ◽  
Author(s):  
K. C. Hall ◽  
D. M. E. Pearce ◽  
M. B. Jackson
Keyword(s):  
Carboxylic Acid ◽  
Plant Tissues ◽  
Mass Selective Detector ◽  
Selective Detector ◽  
Simplified Method

Fusion proteins with both insecticidal and neomycin phosphotransferase II activity

FEBS Letters ◽  
1988 ◽  
Vol 226 (2) ◽  
pp. 364-370 ◽  
Author(s):  
Herman Höfte ◽  
Saskia Buyssens ◽  
Mark Vaeck ◽  
Jan Leemans
Keyword(s):  
Fusion Proteins ◽  
Neomycin Phosphotransferase ◽  
Neomycin Phosphotransferase Ii

Purification and spectrophotometric assay of neomycin phosphotransferase II

1976 ◽  
Vol 69 (1) ◽  
pp. 230-236 ◽  
Author(s):  
Patricia R. Goldman ◽  
Dexter B. Northrop
Keyword(s):  
Spectrophotometric Assay ◽  
Neomycin Phosphotransferase ◽  
Neomycin Phosphotransferase Ii

Purification and characterization of neomycin phosphotransferase II from genetically modified cottonseed (Gossypium hirsutum)

1995 ◽  
Vol 43 (4) ◽  
pp. 1105-1109 ◽  
Author(s):  
David C. Wood ◽  
Linh V. Vu ◽  
Nancee M. Kimack ◽  
Glennon J. Rogan ◽  
Joel E. Ream ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Genetically Modified ◽  
Neomycin Phosphotransferase ◽  
Purification And Characterization ◽  
Neomycin Phosphotransferase Ii
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