DNA typing of HLA in the patients with moyamoya disease

Takuya K. Inoue; Kiyonobu Ikezaki; Takehiko Sasazuki; Takashi Ono; Nobuhiro Kamikawaji; Toshio Matsushima; Masashi Fukui

doi:10.1007/bf02767027

Assessment of extracranial-intracranial bypass in Moyamoya disease using 3T time-of-flight MR angiography: Comparison with CT angiography

VASA ◽

10.1024/0301-1526/a000363 ◽

2014 ◽

Vol 43 (4) ◽

pp. 278-283 ◽

Cited By ~ 8

Author(s):

Qian Chen ◽

Rongfeng Qi ◽

Xiaoqing Cheng ◽

Changsheng Zhou ◽

Song Luo ◽

...

Keyword(s):

Image Quality ◽

Moyamoya Disease ◽

Mr Angiography ◽

Time Of Flight ◽

Consecutive Series ◽

Tof Mra ◽

Bypass Patency ◽

Significant Difference ◽

Contrast Technique ◽

Vessel Integrity

Background: To evaluate the value of time-of-flight MR angiography (TOF MRA) for the assessment of extracranial-intracranial (EC-IC) bypass in Moyamoya disease in comparison with computed tomography angiography (CTA). Patients and methods: A consecutive series of 23 patients with Moyamoya disease were analyzed retrospectively. Twenty three patients underwent 25 procedures of extracranial-intracranial bypass. Cranial CTA was performed within one week after the surgery to assess bypass patency. Then TOF MRA was scanned within 24 h after CTA on a 3T MRI system. Using 5-point scales (0 = poor to 4 = excellent), two radiologists rated the image quality and vessel integrity of bypass for three segments (extracranial, trepanation, intracranial). Results: Image quality was high in both CTA and TOF MRA (mean quality score 3.84 ± 0.37 and 3.8 ± 0.41), without statistical difference (p = 0.66). Mean scores of TOF MRA with respect to bypass visualization were higher than CTA in the intracranial segment (p = 0.026). No significant difference of bypass visualization regarding the extracranial and trepanation segments was found between TOF MRA and CTA (p = 0.66 and p = 0.34, respectively). For the trepanation segment, TOF MRA showed pseudo lesions in 2 of all 25 cases. Conclusions: 3T TOF MRA, a non-contrast technique not exposing the patients to radiation, proved to be at least equal to CTA for the assessment of EC-IC bypass, and even superior to CTA with respect to the intracranial segment. In addition, readers should be aware of a potential overestimation showing focal pseudo lesions of the bypass at the trepanation segment in TOF MRA.

Download Full-text

Changes in superficial temporal artery blood flow and cerebral hemodynamics of moyamoya disease after extracranial-intracranial bypass surgery

Journal of Cerebral Blood Flow & Metabolism ◽

10.1038/sj.jcbfm.9591524.0120 ◽

2005 ◽

Vol 25 (1_suppl) ◽

pp. S120-S120 ◽

Cited By ~ 1

Author(s):

Shigeru Fujimoto ◽

Kazunori Toyoda ◽

Tooru Inoue ◽

Yoko Yokoyama ◽

Juro Jinnouchi ◽

...

Keyword(s):

Blood Flow ◽

Moyamoya Disease ◽

Bypass Surgery ◽

Superficial Temporal Artery ◽

Temporal Artery ◽

Cerebral Hemodynamics ◽

Artery Blood Flow

Download Full-text

Quantitative tomographic evaluation of cerebral perfusion with perfusion weighted magnetic resonance imaging in the patients with moyamoya disease

Journal of Cerebral Blood Flow & Metabolism ◽

10.1038/sj.jcbfm.9591524.0339 ◽

2005 ◽

Vol 25 (1_suppl) ◽

pp. S339-S339

Author(s):

Yoji Tanaka ◽

Tadashi Nariai ◽

Tsukasa Nagaoka ◽

Kiichi Ishiwata ◽

Michio Senda ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Moyamoya Disease ◽

Cerebral Perfusion ◽

Resonance Imaging ◽

Weighted Magnetic Resonance Imaging

Download Full-text

Moyamoya Disease

10.1055/b-002-91663 ◽

2014 ◽

Keyword(s):

Moyamoya Disease

Download Full-text

Localization of the Br Polymorphism on a 144 bp Exon of the GPIa Gene and Its Application in Platelet DNA Typing

Thrombosis and Haemostasis ◽

10.1055/s-0038-1642498 ◽

1994 ◽

Vol 71 (05) ◽

pp. 651-654 ◽

Cited By ~ 50

Author(s):

Rainer Kalb ◽

Sentot Santoso ◽

Katja Unkelbach ◽

Volker Kiefel ◽

Christian Mueller-Eckhardt

Keyword(s):

Genomic Organization ◽

Fragment Length Polymorphism ◽

Human Platelet ◽

Dna Typing ◽

Rflp Analysis ◽

Serological Typing ◽

Allele Specific ◽

Polymerase Chain ◽

Alloimmune Thrombocytopenia ◽

Rflp Typing

SummaryAlloimmunization against the human platelet alloantigen system Br (HPA-5) is the second most common cause of neonatal alloimmune thrombocytopenia (NAIT) in Caucasian populations. We have recently shown that a single base polymorphism at position 1648 on platelet mRNA coding for GPIa results in an aminoacid substitution at position 505 on the mature GPIa which is associated with the two serological defined Br phenotypes.Since DNA-typing of platelet alloantigens offers possibilities for useful clinical applications, we designed genomic DNA-based restriction fragment length polymorphism (RFLP) typing for Br alloantigens. To establish this technique we analyzed the genomic organization of GPIa adjacent to the polymorphic base. Using the polymerase chain reaction (PCR) of blood cell DNA we have identified two introns (approximately 1.7 and 1.9 kb) flanking a 144 bp coding sequence of the GPIa gene encompassing the polymorphic base 1648. Based on the in- tron sequence, a PCR primer was constructed to amplify a 274 bp fragment which was used for allele-specific RFLP to determine the Br genotypes. The results of RFLP analysis using Mnll endonuclease obtained from 15 donors (2 Br37*, 2 Br^ and 11 Brb/b) correlate perfectly with serological typing by monoclonal antibody-specific immobilization of platelet antigens (MAIPA) assay.

Download Full-text