Effects of cholecystokinin-octapeptide on the human gallbladder both in vivo and in vitro

1986 ◽  
Vol 21 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Toku Takahashi ◽  
Takehira Yamamura ◽  
Yoshio Ishikawa ◽  
Masaru Kantoh ◽  
Joji Utsunomiya
Keyword(s):  
Human Gallbladder ◽  
Cholecystokinin Octapeptide

In vivo and in vitro Effects of Cholecystokinin Octapeptide on the Release of β-Endorphin-Like Immunoreactivity

1983 ◽  
Vol 36 (6) ◽  
pp. 443-448 ◽  
Author(s):  
Mitsuhiro Matsumura ◽  
Akira Yamanoi ◽  
Shigeru Yamamoto ◽  
Shiro Saito
Keyword(s):  
Cholecystokinin Octapeptide ◽  
In Vitro Effects

scholarly journals Pretreatment with Gemcitabine/5-Fluorouracil Enhances the Cytotoxicity of Trastuzumab to HER2-Negative Human Gallbladder Cancer Cells In Vitro and In Vivo

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Wei Wang ◽  
Zhenhua Hu ◽  
Yu Huang ◽  
Huilin Zheng ◽  
Qiang Sun ◽  
...  
Keyword(s):  
Gallbladder Cancer ◽  
In Vitro Study ◽  
Sequential Therapy ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Her2 Gene ◽  
Her2 Positive ◽  
Human Gallbladder ◽  
Phosphorylated Akt

The effects of standard clinical therapies including surgery and chemotherapy are poor in advanced gallbladder cancer (GBC). There are a few reported cases of human epidermal growth factor receptor 2 (HER2)-positive GBC that responded well to trastuzumab. But trastuzumab has not yet been used to treat HER2-negative GBC. In this study, we investigated the cytotoxic effects of different combined therapies with trastuzumab and gemcitabine and/or 5-fluorouracil on HER2-negative GBC cell lines in vitro and in vivo. Trastuzumab alone showed almost no cytotoxicity to GBC cells with originally low HER2 gene amplification. Sequential therapy with chemotherapy followed by trastuzumab showed superiority over reverse sequential chemotherapy (P<0.05), concurrent combined chemotherapy (P<0.05), chemotherapy alone (P<0.05), and trastuzumab alone (P<0.05) in terms of cytotoxicity. Sequential therapy with chemotherapy followed by trastuzumab nearly completely inhibited cell viability in HER2-negative GBC cells. Similar results were observed with regard to apoptosis. Western blot analysis showed that gemcitabine/5-fluorouracil increased the expressions of total and phosphorylated forms of HER2, thus enhancing the cytotoxicity of trastuzumab. In vivo study verified the results of in vitro study by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling assay and immunohistochemical analysis. Moreover, not only the lightest tumor bearing but also the best survival state was detected in sequential therapy with chemotherapy followed by trastuzumab group compared with other groups. Our in vivo and in vitro data suggest that sequential therapy with gemcitabine/5-fluorouracil followed by trastuzumab represents a novel and promising therapeutic strategy against HER2-negative GBC. The upregulation of phosphorylated HER2 and phosphorylated-AKT induced by gemcitabine/5-fluorouracil treatment shows that HER2/AKT pathway is triggered.

scholarly journals Downregulation of stathmin 1 in human gallbladder carcinoma inhibits tumor growth in vitro and in vivo

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jiwen Wang ◽  
Yanli Yao ◽  
Yue Ming ◽  
Sheng Shen ◽  
Nan Wu ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Gallbladder Carcinoma ◽  
Human Gallbladder ◽  
Stathmin 1

Interaction between secretin and cholecystokinin-octapeptide in the exocrine rat pancreas in vivo and in vitro

1992 ◽  
Vol 77 (1) ◽  
pp. 191-204 ◽  
Author(s):  
J Singh ◽  
R Lennard ◽  
GM Salido ◽  
D Wisdom ◽  
CL Render ◽  
...  
Keyword(s):  
Rat Pancreas ◽  
Cholecystokinin Octapeptide

Sulfated cholecystokinin octapeptide in the rat: pontomedullary distribution and modulation of the respiratory pattern

1999 ◽  
Vol 77 (7) ◽  
pp. 490-504 ◽  
Author(s):  
Howard H Ellenberger ◽  
Frank M Smith
Keyword(s):  
Respiratory Rhythm ◽  
Mean Amplitude ◽  
Neonatal Rat ◽  
Respiratory Pattern ◽  
Reticular Nucleus ◽  
Adult Rats ◽  
Cholecystokinin Octapeptide ◽  
Ventral Respiratory Group

We performed anatomical and physiological studies to determine the site and actions of sulfated cholecystokinin octapeptide (CCK8-S) on breathing. Peptide locations were determined by combined immunodetection of CCK8-S- containing synaptic varicosities and retrograde labeling of medullary neurons projecting to the ventral respiratory group. Retrogradely labeled neurons and CCK8-S immunolabeled varicosities overlapped within the nuclei of the solitary tract, ventral respiratory group, and the Kölliker-Fuse nucleus. Additional CCK8-S immunoreactive terminals were located in the rostroventrolateral medullary reticular nucleus, lateral paragigantocellular reticular nucleus, and the caudal pontine reticular nucleus. The respiratory effects of CCK8-S, which binds to CCKA and CCKB receptors, were examined by intravenous injection in adult rats and by bath application in the in vitro neonatal rat brainstem - spinal cord preparation. CCK8-S produced an increase in the mean amplitude of diaphragmatic electromyogram (EMG) of 28 ± 35% (SD) and a decrease in mean respiratory interval of 13 ± 4% in vivo. In vitro, CCK8-S significantly increased inspiratory duration and decreased respiratory interval, primarily by shortening expiratory duration. CCK8-unsulfated, a specific agonist for CCKB receptors, did not produce these effects. CCK8-S effects in the in vitro preparation were partially blocked by the CCK receptor antagonist lorglumide (final bath concentration 600 nM). These results suggest that CCK8-S modulates the respiratory rhythm via CCKA receptors within one or more medullary or pontine respiratory groups in both neonatal and adult rats.Key words: neuropeptide, ventral respiratory group, medulla, pons, respiratory network.

A Generalized Hermite Pyramid for Ultrasonic Image Analysis II. Gallstones

1996 ◽  
Vol 18 (4) ◽  
pp. 305-327
Author(s):  
Y. V. Venkatesh
Keyword(s):  
Image Analysis ◽  
Chemical Composition ◽  
Ultrasonic Imaging ◽  
Decomposition Technique ◽  
Human Gallbladder ◽  
Ultrasonic Image ◽  
Ultrasonic Images

This paper deals with the problem of extracting information regarding the chemical composition of stones in the human gallbladder from in vitro and in vivo B-scan ultrasonic images. The images are subjected to the Hermite pyramid decomposition technique described in Part I (Venkatesh, Y. V., Ultrasonic Imaging, 18, 261–304, 1996). In an attempt to determine the chemical composition of the gallstones, the gradients of the decomposed images are input to an unsupervised classifier. The outputs of the classifier exhibit some interesting patterns that appear to be related to the chemical composition of the gallstones contained in these images.

In Vivo and In Vitro Effects of Cholecystokinin Octapeptide on the Release of Prolactin in Rats

1985 ◽  
Vol 17 (06) ◽  
pp. 293-297 ◽  
Author(s):  
M. Matsumura ◽  
A. Yamanoi ◽  
S. Yamamoto ◽  
H. Mori ◽  
S. Saito
Keyword(s):  
Cholecystokinin Octapeptide ◽  
In Vitro Effects

Cholecystokinin octapeptide excites dorsal horn neurons both in vivo and in vitro

1981 ◽  
Vol 213 (1) ◽  
pp. 231-236 ◽  
Author(s):  
Srdija Jeftinija ◽  
Vjekoslav Miletić ◽  
Mirjana Randić
Keyword(s):  
Dorsal Horn ◽  
Cholecystokinin Octapeptide ◽  
Dorsal Horn Neurons
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