Factors controlling adventitious bud induction and plant regeneration in matureJuniperus oxycedrus leaves cultured in vitro

1994 ◽  
Vol 30 (4) ◽  
pp. 210-218 ◽  
Author(s):  
M. P. Gomez ◽  
J. Segura
Keyword(s):  
Plant Regeneration ◽  
Adventitious Bud ◽  
Bud Induction ◽  
Adventitious Bud Induction

scholarly journals Basal stem cluster bud induction and efficient regeneration for the Tibetan endemic medicinal plant Swertia conaensis

2021 ◽  
Vol 49 (2) ◽  
pp. 12152
Author(s):  
Yin-Kai XI ◽  
Heng-Yu HUANG
Keyword(s):  
Plant Regeneration ◽  
Medicinal Plant ◽  
Orthogonal Experiment ◽  
Occurrence Rate ◽  
Adventitious Bud ◽  
Ms Medium ◽  
Regeneration System ◽  
Bud Induction ◽  
Endemic Medicinal Plant

The artificial rapid propagation system for Swertia conaensis T. N. Ho et S. W. Liu was explored to screen the appropriate plant regeneration method and to provide an efficient propagation mode, useful for artificial breeding technology or for further research and development of the Tibetan endemic medicinal plant. In this study, the most suitable explant and hormone were chosen according to single factor test. Next, the effects of different hormone combinations on basal stem cluster bud induction, callus induction, adventitious bud occurrence and plant regeneration were investigated by using complete combination and orthogonal experiment. The obtained results showed that the explants suitable for in vitro of S. conaensis were stem tips with leaves, which were regenerated through the method of basal stem cluster bud occurrence in the MS medium with 2.0 mg∙L-1 6-BA, 0.5 mg∙L-1 NAA, but the proliferation coefficient was low, only 3.16 after 40 days of culture. Subsequently, the proliferation coefficient failed to improve, irrespective of change of the concentration ratio of 6-BA and NAA. Therefore, in the orthogonal experiment of adding ZT, the MS medium with 1.0 mg∙L-1 ZT, 0.5 mg∙L-1 NAA and 2.5 mg∙L-1 6-BA induced a large number of callus green and compact, with 86.30% callus occurrence rate. After 40 days of culture, the rate of adventitious bud occurrence was 96.55% and the proliferation coefficient was high (10.37). The rooting rate was 100% in the 1/2MS medium with 0.5 mg∙L-1 NAA. The survival rate of regenerated plants was more than 95%. Indirect organogenesis was more efficient than direct organogenesis in in vitro culture of S. conaensis. In this study, the efficient and stable regeneration system of S. conaensis was achieved through the method of explant to callus to adventitious buds, which provided an effective way to an endangered species.

Factors affecting adventitious bud induction in Pinus elliottii (Engelm.) embryos cultured in vitro

1987 ◽  
Vol 11 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Pedro P�rez-Berm�dez ◽  
Harry E. Sommer
Keyword(s):  
Pinus Elliottii ◽  
Adventitious Bud ◽  
Factors Affecting ◽  
Bud Induction ◽  
Adventitious Bud Induction

ZYGOTIC EMBRYO CULTURE OF TAXUS CHINESIS VAR. MAIREI AND PLANT REGENERATION THROUGH ORGANOGENESIS

1999 ◽  
Vol 47 (4) ◽  
pp. 287-289 ◽  
Author(s):  
Deyu Xie ◽  
Zhongchen Guo
Keyword(s):  
Plant Regeneration ◽  
Embryo Culture ◽  
Adventitious Roots ◽  
Zygotic Embryo ◽  
Adventitious Bud ◽  
Ms Medium ◽  
Adventitious Buds ◽  
Zygotic Embryo Culture ◽  
Bud Induction ◽  
Adventitious Bud Induction

When different media were compared for their effect on zygotic embryo culture, DCR was found more effective than MS medium. From these embryos, adventitious bud induction was achieved by optimizing formular conditions as described in this paper. Adventitious buds were formed from calli derived from hypocotyls of germinated embryos. Finally, adventitious roots were induced and complete plantlets were obtained.

Disease-resistant transgenic adzuki bean plants obtained through an efficient transformation system

2012 ◽  
Vol 63 (12) ◽  
pp. 1090 ◽  
Author(s):  
Huatao Chen ◽  
Xin Chen ◽  
Heping Gu ◽  
Xingxing Yuan ◽  
Hongmei Zhang ◽  
...  
Keyword(s):  
Soybean Mosaic Virus ◽  
Shoot Elongation ◽  
Zeatin Riboside ◽  
Medium Composition ◽  
Adventitious Bud ◽  
Adzuki Bean ◽  
Transformation System ◽  
Bean Plants ◽  
Bud Induction

An efficient regeneration and transformation system was established and optimised for adzuki bean (Vigna angularis (Willd.) Ohwi & Ohashi). 6-Benzylaminopurine at 5 mg L–1 was used to increase adventitious bud induction frequency. The highest frequency of shoot elongation was 92.8% when using a medium composition of MS salts combined with 0.1 mg L–1 of IAA, 0.5 mg L–1 of GA3, 1.0 mg L–1 of zeatin-riboside, 50 mg L–1 of aspartic acid, and 50 mg L–1 of glutamic acid. In vitro rooting was 100% when shoots were cultured on the solid MS medium supplemented with 1.0 mg L–1 of NAA. Reproducible transformation of epicotyl explants was developed using the A. tumefaciens EHA105 strain. Using a concentration of 40 mg L–1 of acetosyringone, 20 mm MES, and 5 mg L–1 of 6-benzylaminopurine in the co-cultivation medium, a transformation efficiency of 12.6% was attained. Using this transformation protocol, we obtained transgenic adzuki bean plants resistant to soybean mosaic virus by introducing the V. angularis VaPR3 gene.

scholarly journals Plant Regeneration from in Vitro Culture of Embryonic Axis Explants in Common and Tepary Beans

1992 ◽  
Vol 117 (2) ◽  
pp. 332-336 ◽  
Author(s):  
Mohamed F. Mohamed ◽  
Paul E. Read ◽  
Dermot P. Coyne
Keyword(s):  
Plant Regeneration ◽  
Common Bean ◽  
Basal Medium ◽  
Continuous Light ◽  
Embryonic Axis ◽  
Naphthaleneacetic Acid ◽  
Tepary Bean ◽  
Bud Induction ◽  
Multiple Buds

A new in vitro protocol was developed for multiple bud induction and plant regeneration from embryonic axis explants of four common bean (Phaseolus vulgaris L.) and two tepary bean (P. acutifolius A. Gray) lines. The explants were prepared from two embryo sizes, 3 to 4 mm and 5 to 7 mm, corresponding to pods collected after 15 and 25 days from flowering, respectively. The embryonic axis was cultured on Gamborg's B5 basal medium with 0, 5, 10, or 20 μm BA in combinations with 0, 1, or 2 μm NAA. The cultures were maintained at 24 to 25C under continuous light or incubated in darkness for 2 weeks followed by continuous light before transfer to the secondary B5 medium (0 or 2 μm BA or 2 μm BA plus 4 μm GA3). Adventitious roots or a single shoot with roots formed on the explants cultured on media without plant growth regulators. Multiple buds were induced on all BA media, but more were produced with 5 or 10 μm for most lines. Dark incubation greatly enhanced multiple bud initiation. Shoot buds were not produced on media containing NAA alone or in combinations with BA. On the secondary medium, six to eight shoots per explant for common bean and up to 20 shoots per explant from tepary bean were observed after 3 weeks. Mature, fertile plants were produced from these shoots. Chemical names used: benzyladenine (BA); 1-naphthaleneacetic acid (NAA); gibberellic acid (GA3).

scholarly journals HISTOLOGICAL EXAMINATION OF IN VITRO ADVENTITIOUS BUD DEVELOPMENT ON HYPOCOTYLS OF FRASER FIR

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1102a-1102
Author(s):  
Carole H. Saravitz ◽  
Frank A. Blazich ◽  
Henry V. Amerson
Keyword(s):  
Growth Regulators ◽  
Induction Medium ◽  
Adventitious Bud ◽  
Naphthaleneacetic Acid ◽  
Abies Fraseri ◽  
Fraser Fir ◽  
Cell Clusters ◽  
Cell Divisions ◽  
Bud Induction

Hypocotyls of Fraser fir (Abies fraseri (Pursh) Poir.) were excised from seeds germination 9 days and placed on bud induction medium containing 10 mg/liter benzyladenine (BA) and 0.01 mg/liter naphthaleneacetic acid (NAA) or medium without growth regulators. After 3 days on medium containing growth regulators, cell divisions were localized in epidermal and subepidermal layers of the hypocotyl while similar cell divisions were not observed in control-treated hypocotyls. Cell clusters consisting of two to five cells were present after 7 days in hypocotyls placed on bud induction medium. In control-treated hypocotyls, stomata continued to develop and cells within the cortex became vacuolated during the first 2 weeks in culture. All hypocotyls were transferred to secondary medium after 3 weeks. Cell clusters continued to enlarge into meristemoids in hypocotyls initially placed on bud induction medium. Gradually, meristemoids developed into buds and cataphylls were observed covering bud meristems.

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