Chemical composition, in vitro larvicidal and antileishmanial activities of the essential oil from Citrus reticulata Blanco fruit peel

Numerous studies have investigated the chemical composition and biological activities of essential oils from different Citrus species fruit peel, leaves and flowers. This paper aims to investigate the chemical composition, larvicidal and antileishmanial activities of essential oil from Citrus reticulata fruit peel (CR-EO). CR-EO was obtained by hydrodistillation in a Clevenger-type apparatus and its chemical composition was analyzed by GC-MS and GC-FID. Limonene (85.7%), ɣ-terpinene (6.7%) and myrcene (2.1%) were identified as its major components. CR-EO showed high activity against promastigote forms of Leishmania amazonensis (IC50 = 8.23 µg/mL). CR-EO also exhibited high larvicidal activity against third instar Aedes aegypti larvae at a lethal concentration (LC50 = 58.35 µg/mL) and 100% mortality at 150 µg/mL. This study suggests, for the first time, the potential use of CR-EO against this important mosquito-borne viral disease caused by the genus Aedes.

EVALUACIÓN DE LA COMERCIALIZACIÓN DEL TANGELO (Citrus reticulata x Citrus paradasi) CON MANEJO POST COSECHA EN EL ANEXO DE SANTA ROSA DE CASHINGARI, SATIPO

Los factores que intervienen en la post cosecha del Tangelo son la organización, extensión agrícola, accesibilidad al crédito agrario y la comercialización. El costo de producción promedio del Tangelo por hectárea sin post cosecha es de S/ 4 064,00 y la utilidad por campaña oscila entre S/ 818,00 a S/1 853,00 con una rentabilidad desde 20,54% hasta 44,68%. El costo de post cosecha promedio del Tangelo por hectárea es de S/18 912,00 con una utilidad desde S/ 4 999,00 hasta S/ 7 459,00 con una rentabilidad de 28,93% hasta 36,38% en la primera campaña. Para la segunda campaña la rentabilidad del tangelo con poscosecha se incrementó 61,49%. Durante el tratamiento post cosecha se obtiene: 5% de descarte, 30% de calidad primera, 45% de calidad segunda y 20% calidad tercera. La presentación del producto es en cajas de cartón corrugado de 40, 50 y 60 unidades según la calidad. Con la contrastación estadística se puede afirmar con 95% de seguridad que la rentabilidad es mayor en el Tangelo con post cosecha a partir de la segunda campaña. Se concluye que con la aplicación del tratamiento post cosecha en el Tangelo se logra incrementar la rentabilidad económica del agricultor el cual tendrá impacto en la ampliación de su frontera agrícola, alimentación, educación, vestimenta y salud.

EVALUACIÓN DE ESPECIES DE MOSCAS DE LA FRUTA Y SUS HOSPEDEROS EN LA ZONA DE SATIPO

Las plantas hospederas de mosca de la fruta fueron evaluados de los fundos de la zona de Satipo, en campo y laboratorio. Los resultados muestran que los cítricos están infestadas por Anastrepha fraterculus; mientras que los frutales nativos con otras especies de Anastrepha, como son A. striata, A. leptozona, y A. distincta. Se ha encontrado un díptero amarillo en el caimito que no pertenece a los tephritidae. Se tiene 19 frutales distintos hospederas, siendo los cítricos C. reticulata x C. aurantifolia, Citrus limettioides, Citrus aurantifolia, Citrus reticulata, Citrus reshni, Citrus sinensis, C. reticulata x C. sinensis, C. reticulata x C. paradisi; y los frutales nativos y exóticos, Chrysophillum caimito, Averrhoa carambola, Spondias sp., Psidium guajaba, Manguifera indica, Inga edulis Mart, Anacardiaceae y Matisia cordata. El uvo de monte, guayaba y caimito sufren un daño de entre 83 Y 100%. El zapote y pacae muestran daños de entre 66 a 53%. El mango y carambola muestran daños de 31 a 26%. En los cítricos, la naranja Washington muestra los niveles más altos de infestación con 45%,; las mandarinas entre 27 y 32%, las naranjas entre 14 y 22%, los híbridos entre 20 y 22%, los portainjertos entre 26 y 27%. Las pérdidas económicas provinciales del 14% por moscas de la fruta sólo en naranja Valencia se estima en U.S.$ 2,025,000,00. Palabras clave: Anastrepha, fraterculus, striata, cítricos, Satipo,

Enumeration of Citrus endophytic bacterial communities based on illumine metagenomics technique

Citrus is a valuable crop in Pakistan because it is rich in vitamin C and antioxidants. Huanglongbing (HLB) has an influence on citrus production around the world caused by a bacterium “ Candidatus liberibacter asiaticus ” (CLas), africanus and americanus. The structure and diversity of bacterial species in various ecosystems can be quickly examined using NGS. This approach is considerably quicker and more precise than outdated methods. Healthy or citrus greening infected leaf samples of Grapefruit, Citrus aurantifolia , and Citrus reticulata Blanco was used for diversity analysis. In this study high throughput, NGS technique was used to access the population of both cultivable and non-cultivable bacterial endophytes from citrus leaves, by using PCR amplicons of 16S rDNA sequences (V5–V7 regions) with Illumina Hi seq. As a result, a total number of 68,722 sequences were produced from the test samples. According to the NGS-based diversity classification, the most common genera of exploited bacterial endophytes were Proteobacteria, Firmicutes, Bacteroides, Cyanobacteria, and Actinobacteria. Citrus aurantifolia and Citrus paradisi showed almost equal diversity, whereas Citrus reticulata Blanco had a higher proportion of Proteobacteria and Cyanobacteria in their leaves. To determine alpha diversity (AD), additional data was analyzed using statistical indices such as Shannon, Chao1, and Simpson. According to the inverse Simpson diversity index, the abundance of the microbial population in six different citrus samples was 0.48, 0.567, and 0.163, respectively. The metagenomics of microbiota in plant tissues was successfully recorded by NGS technology, which can help us learn more about the interactions between plants and microbes. This research is the first step toward a better understanding of 16SrRNA-based metagenomics from citrus in Pakistan using Illumina (Hi seq) Technology.

Effect of a Carotenoid Extract from Citrus reticulata By-Products on the Immune-Oxidative Status of Broilers

Although carotenoids generally possess antimicrobial and antioxidant properties, the in vivo synergistic action of carotenoid blends derived from plant-based by-products has not been thoroughly studied. Therefore, the carotenoid characterization and antimicrobial potential of Citrus reticulata extract as well as the impact of this carotenoid-rich extract (CCE) dietary supplementation on the performance, meat quality, and immune-oxidative status of broiler chickens were determined. One hundred and twenty one-day-old hatched chicks (Ross 308) were allocated to two dietary groups, with four replicate pens of 15 birds each. Birds were fed either a basal diet (CON) or the basal diet supplemented with 0.1% CCE (25 mg carotenoid extract included in 1 g of soluble starch) for 42 d. β-Cryptoxanthin, β -Carotene, Zeaxanthin, and Lutein were the prevailing carotenoid compounds in the Citrus reticulata extract. The CCE feed additive exerted inhibitory properties against both Gram-positive (Staphylococcus aureus) and negative (Klebsiella oxytoca, Escherichia coli, and Salmonella typhimurium) bacteria. Both the broiler performance and meat quality did not substantially differ, while the breast muscle malondialdehyde (MDA) concentration tended to decrease (p = 0.070) in the CCE-fed broilers. The inclusion of CCE decreased the alanine aminotransferase and MDA concentration, and the activity of glutathione peroxidase, while the activity of superoxide dismutase was increased in the blood. Catalase and NADPH oxidase 2 relative transcript levels were significantly downregulated in the livers of the CCE-fed broilers. Additionally, Interleukin 1β and tumor necrosis factor (TNF) relative transcript levels were downregulated in the livers of the CCE- fed broilers, while TNF and interferon γ (IFNG) tended to decrease in the spleens and bursa of Fabricius, respectively. The present study provided new insights regarding the beneficial properties of carotenoids contained in Citrus reticulata in broilers’ immune-oxidative status. These promising outcomes could be the basis for further research under field conditions.

Network pharmacology study of Citrus reticulata and Pinellia ternata in the treatment of non-small cell lung cancer

It has been recognized that Citrus reticulata and Pinellia ternata have a good therapeutic effect on NSCLC. However, the potential mechanism of C. reticulata and P. ternata in the treatment of NSCLC based on network pharmacology analysis is not clear. The “Drug-Component-Target-Disease” network was constructed by Cytoscape, and the protein interaction (PPI) network was constructed by STRING. Our study indicated that 18 active ingredients of C. reticulata and P. Ternata were screened from the TCMSP database, and 56 target genes of C. reticulata and P. Ternata for the treatment of NSCLC were identified, and we constructed the “Drug-Component-Target-Disease” network. In this study, we screened 56 PPI core genes to establish a PPI network. We concluded that the network pharmacology mechanism of the effect of C. reticulata and P. Ternata on NSCLC may be closely related to the protein expressed by TP53, ESR1, FOS, NCOA3 and MAPK8, and these may play the therapeutic roles by regulating the IL-17 signaling pathway, antigen processing and presentation, microRNAs in cancer and endocrine resistance.