Multiplex detection ofPotato virus S, Potato virus X, andPotato virus Y by non-radioactive nucleic acid spot hybridization in potato tissue culture plantlets

2006 ◽  
Vol 83 (6) ◽  
pp. 495-501 ◽  
Author(s):  
Katarzyna L. Janczur ◽  
Mark K. Nakhla ◽  
Amy O. Charkowski
Keyword(s):  
Tissue Culture ◽  
Nucleic Acid ◽  
Potato Virus ◽  
Potato Virus X ◽  
Multiplex Detection ◽  
Potato Tissue ◽  
Spot Hybridization ◽  
Nucleic Acid Spot Hybridization ◽  
Tissue Culture Plantlets

Detection of Huanglongbing (Citrus Greening) Disease by Nucleic Acid Spot Hybridization

2009 ◽  
Vol 64 (9-10) ◽  
pp. 711-716 ◽  
Author(s):  
Kuraba Gopal ◽  
Sundeep Sudarsan ◽  
Venati Gopi ◽  
Latchireddy Naram Naidu ◽  
Maniyaram Ramaiah ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sweet Orange ◽  
Pcr Amplification ◽  
Positive Control ◽  
Infected Tissue ◽  
Citrus Greening Disease ◽  
Pcr Product ◽  
Spot Hybridization ◽  
Total Dna ◽  
Nucleic Acid Spot Hybridization

Polymerase chain reaction (PCR) amplification with primers specific to the rDNA region successfully amplified the 1160-bp DNA fragment from a Huanglongbing (HLB)-infected sweet orange sample with mottling symptoms leaves, but not from healthy sweet orange plants. The PCR product of 1160-bp was used as probe labeled with biotin for detection of the HLB pathogen in the nucleic acid spot hybridization (NASH) test. It was found that the HLB pathogen could be detected up to 1:100 dilution in HLB-infected tissue. Total DNA extracted from HLB-infected tissue was diluted 2-fold as 900 ng in TE buffer and spotted on a nitrocellulose membrane. Strong signals were observed up to 225 ng of DNA dilution, whereas a moderate signal was recorded at 112 ng. No hybridization signal was observed in the healthy samples, while strong signals were observed in the positive control

Typing of herpes simplex virus isolates with monoclonal antibodies and by nucleic acid spot hybridization

1985 ◽  
Vol 12 (1-2) ◽  
pp. 169-177 ◽  
Author(s):  
Thedi Ziegler ◽  
Veijo Hukkanen ◽  
Pertti Arstila ◽  
Petri Auvinen ◽  
Annika Jalava ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Nucleic Acid ◽  
Spot Hybridization ◽  
Simplex Virus ◽  
Nucleic Acid Spot Hybridization ◽  
Virus Isolates
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