Relationship of intracellular free Ca2+ concentration and calcium-activated chloride channels of pulmonary artery smooth muscle cells in rats under hypoxic conditions

2006 ◽  
Vol 26 (2) ◽  
pp. 172-174 ◽  
Author(s):  
Yang Zhao ◽  
Zhang Zhenxiang ◽  
Xu Yongjian ◽  
Li Yaqing ◽  
Ye Tao
Keyword(s):  
Smooth Muscle ◽  
Pulmonary Artery ◽  
Smooth Muscle Cells ◽  
Chloride Channels ◽  
Muscle Cells ◽  
Hypoxic Conditions ◽  
Pulmonary Artery Smooth Muscle ◽  
Relationship Of

Cerebrosides of baifuzi, a novel potential blocker of calcium-activated chloride channels in rat pulmonary artery smooth muscle cells

2007 ◽  
Vol 31 (9) ◽  
pp. 908-915 ◽  
Author(s):  
Shang-bang Gao ◽  
Chang-ming Wang ◽  
Xue-song Chen ◽  
Wei-wei Yu ◽  
Bi-wen Mo ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Pulmonary Artery ◽  
Smooth Muscle Cells ◽  
Chloride Channels ◽  
Muscle Cells ◽  
Pulmonary Artery Smooth Muscle

scholarly journals Hsa_circ_0002062 Promotes the Proliferation of Pulmonary Artery Smooth Muscle Cells by Regulating the Hsa-miR-942-5p/CDK6 Signaling Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Yali Wang ◽  
Xiaoming Tan ◽  
Yunjiang Wu ◽  
Sipei Cao ◽  
Yueyan Lou ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Pulmonary Artery ◽  
Smooth Muscle Cells ◽  
Target Genes ◽  
Rna Binding ◽  
Muscle Cells ◽  
Hypoxic Conditions ◽  
Competing Endogenous Rnas ◽  
Pulmonary Artery Smooth Muscle

Currently, new strategies for the diagnosis and treatment of hypoxia-induced pulmonary hypertension (HPH) are urgently required. The unique features of circRNAs have unveiled a novel perspective for understanding the biological mechanisms underlying HPH and the possibility for innovative strategies for treatment of HPH. CircRNAs function as competing endogenous RNAs (CeRNA) to sequester miRNAs and regulate the expression of target genes. This study aimed to explore the roles of hsa_circ_0002062 on the biological behaviors of pulmonary artery smooth muscle cells (PASMCs) in hypoxic conditions. A number of in vitro assays, such as RNA-binding protein immunoprecipitation (RIP), RNA pull-down, and dual-luciferase assays were performed to evaluate the interrelationship between hsa_circ_0002062, hsa-miR-942-5P, and CDK6. The potential physiological functions of hsa_circ_0002062, hsa-miR-942-5P, and CDK6 in hypoxic PASMCs were investigated through expression modulation. Our experiments demonstrated that hsa_circ_0002062 functions as a ceRNA, acts as a sponge for hsa-miR-942-5P, and consequently activates CDK6, which further promotes pulmonary vascular remodeling. Therefore, we speculate that hsa_circ_0002062 could serve as a candidate diagnostic biomarker and potential therapeutic target for HPH.

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