Cloning and characterization of a cDNA encoding glyceral-dehyde-3-phosphate dehydrogenase from the fission yeast Schizosaccharomyces pombe

1996 ◽  
Vol 7 (4) ◽  
pp. 315-322 ◽  
Author(s):  
Ivan Orlandi ◽  
Laura Popolo ◽  
Paola Cavadini ◽  
Marina Vai ◽  
E. Marrè
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast

Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

Characterization of Cu, Zn-superoxide dismutase-deficient mutant of fission yeast Schizosaccharomyces pombe

2002 ◽  
Vol 41 (2) ◽  
pp. 82-88 ◽  
Author(s):  
Norihiro Mutoh ◽  
Chiaki Nakagawa ◽  
Kenichiro Yamada
Keyword(s):  
Superoxide Dismutase ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Deficient Mutant

Isolation and characterization of hrp1 +, a new member of the SNF2/SWI2 gene family from the fission yeast Schizosaccharomyces pombe

1998 ◽  
Vol 257 (3) ◽  
pp. 319-329 ◽  
Author(s):  
Y. H. Jin ◽  
E. J. Yoo ◽  
Y. K. Jang ◽  
S. H. Kim ◽  
M. J. Kim ◽  
...  
Keyword(s):  
Gene Family ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Isolation And Characterization

scholarly journals Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3.

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905 ◽  
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

Physiological characterization of a cadmium-resistant mutant in the fission yeast Schizosaccharomyces pombe

1995 ◽  
Vol 150 (3) ◽  
pp. 233-237 ◽  
Author(s):  
Christian Wunderlich ◽  
Qingshi Zhao ◽  
Martin Zimmermann ◽  
Klaus Wolf
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Resistant Mutant ◽  
Physiological Characterization
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