Characterization of Cu, Zn-superoxide dismutase-deficient mutant of fission yeast Schizosaccharomyces pombe

2002 ◽  
Vol 41 (2) ◽  
pp. 82-88 ◽  
Author(s):  
Norihiro Mutoh ◽  
Chiaki Nakagawa ◽  
Kenichiro Yamada
Keyword(s):  
Superoxide Dismutase ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Deficient Mutant

Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

scholarly journals Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3.

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905 ◽  
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

Cloning and characterization of a cDNA encoding glyceral-dehyde-3-phosphate dehydrogenase from the fission yeast Schizosaccharomyces pombe

1996 ◽  
Vol 7 (4) ◽  
pp. 315-322 ◽  
Author(s):  
Ivan Orlandi ◽  
Laura Popolo ◽  
Paola Cavadini ◽  
Marina Vai ◽  
E. Marrè
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast

Physiological characterization of a cadmium-resistant mutant in the fission yeast Schizosaccharomyces pombe

1995 ◽  
Vol 150 (3) ◽  
pp. 233-237 ◽  
Author(s):  
Christian Wunderlich ◽  
Qingshi Zhao ◽  
Martin Zimmermann ◽  
Klaus Wolf
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Resistant Mutant ◽  
Physiological Characterization
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