Development of selection marker-free transgenic potato plants with enhanced tolerance to oxidative stress

2008 ◽  
Vol 51 (6) ◽  
pp. 401-407 ◽  
Author(s):  
Raza Ahmad ◽  
Yun-Hee Kim ◽  
Myoung Duck Kim ◽  
Minh-Ngoc Phung ◽  
Won-Il Chung ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transgenic Potato ◽  
Selection Marker ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Marker Free

scholarly journals Spread of Recombinant DNA by Roots and Pollen of Transgenic Potato Plants, Identified by Highly Specific Biomonitoring Using Natural Transformation of an Acinetobacter sp

2003 ◽  
Vol 69 (8) ◽  
pp. 4455-4462 ◽  
Author(s):  
Johann de Vries ◽  
Martin Heine ◽  
Klaus Harms ◽  
Wilfried Wackernagel
Keyword(s):  
Recombinant Dna ◽  
Natural Transformation ◽  
High Sensitivity ◽  
Transgenic Potato ◽  
Kanamycin Resistance ◽  
Selection Marker ◽  
Potato Plants ◽  
Gene Coding ◽  
Transgenic Potato Plants ◽  
Field Plots

ABSTRACT Transgenic potato plants with the nptII gene coding for neomycin phosphotransferase (kanamycin resistance) as a selection marker were examined for the spread of recombinant DNA into the environment. We used the recombinant fusion of nptII with the tg4 terminator for a novel biomonitoring technique. This depended on natural transformation of Acinetobacter sp. strain BD413 cells having in their genomes a terminally truncated nptII gene (nptII′; kanamycin sensitivity) followed by the tg4 terminator. Integration of the recombinant fusion DNA by homologous recombination in nptII′ and tg4 restored nptII, leading to kanamycin-resistant transformants. DNA of the transgenic potato was detectable with high sensitivity, while no transformants were obtained with the DNA of other transgenic plants harboring nptII in different genetic contexts. The recombinant DNA was frequently found in rhizosphere extracts of transgenic potato plants from field plots. In a series of field plot and greenhouse experiments we identified two sources of this DNA: spread by roots during plant growth and by pollen during flowering. Both sources also contributed to the spread of the transgene into the rhizospheres of nontransgenic plants in the vicinity. The longest persistence of transforming DNA in field soil was observed with soil from a potato field in 1997 sampled in the following year in April and then stored moist at 4°C in the dark for 4 years prior to extract preparation and transformation. In this study natural transformation is used as a reliable laboratory technique to detect recombinant DNA but is not used for monitoring horizontal gene transfer in the environment.

scholarly journals Generation of double-virus-resistant marker-free transgenic potato plants

2009 ◽  
Vol 19 (5) ◽  
pp. 543-548 ◽  
Author(s):  
Yunfeng Bai ◽  
Zhihua Guo ◽  
Xiaoqi Wang ◽  
Dongmei Bai ◽  
Weifeng Zhang
Keyword(s):  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Marker Free

Enhanced tolerance to methyl viologen-induced oxidative stress and high temperature in transgenic potato plants overexpressing the CuZnSOD, APX and NDPK2 genes

2010 ◽  
Vol 140 (2) ◽  
pp. 153-162 ◽  
Author(s):  
Myoung Duck Kim ◽  
Yun-Hee Kim ◽  
Suk-Yoon Kwon ◽  
Dae-Jin Yun ◽  
Sang-Soo Kwak ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Temperature ◽  
Methyl Viologen ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants

Sucrose synthase activity does not restrict glycolysis in roots of transgenic potato plants under hypoxic conditions

Planta ◽  
1999 ◽  
Vol 210 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Sophia Biemelt ◽  
Mohammad Reza Hajirezaei ◽  
Michael Melzer ◽  
Gerd Albrecht ◽  
Uwe Sonnewald
Keyword(s):  
Sucrose Synthase ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Hypoxic Conditions ◽  
Transgenic Potato Plants

scholarly journals Metabolism of Herbicides and Other Chemicals in Human Cytochrome P450 Species and in Transgenic Potato Plants Co-Expressing Human CYP1A1, CYP2B6 and CYP2C19

2001 ◽  
Vol 26 (1) ◽  
pp. 28-40 ◽  
Author(s):  
Hideyuki INUI ◽  
Noriaki SHIOTA ◽  
Yukiko MOTOI ◽  
Yoshiko IDO ◽  
Tomomi INOUE ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Human Cytochrome ◽  
Transgenic Potato Plants

Transgenic potato plants expressing the nptII-gus marker genes affect survival and development of the Colorado potato beetle

Plant Science ◽  
2002 ◽  
Vol 162 (3) ◽  
pp. 373-380 ◽  
Author(s):  
Sébastien De Turck ◽  
Philippe Giordanengo ◽  
Anas Cherqui ◽  
Corinne Ducrocq-Assaf ◽  
Brigitte S Sangwan-Norreel
Keyword(s):  
Colorado Potato Beetle ◽  
Transgenic Potato ◽  
Marker Genes ◽  
Potato Plants ◽  
Potato Beetle ◽  
Survival And Development ◽  
Transgenic Potato Plants
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