Application of arsenazo III for the polarographic detection of proteins

2008 ◽  
Vol 5 (1) ◽  
pp. 115-121 ◽  
Author(s):  
W. Sun ◽  
N. Zhao ◽  
B. Xu ◽  
M. N. Wang ◽  
K. Jiao
Keyword(s):  
Arsenazo Iii ◽  
Polarographic Detection

Passive micromixer integration with a microfluidic chip for calcium assay based on the arsenazo III method

2011 ◽  
Vol 5 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Yuwadee Boonyasit ◽  
Thitima Maturos ◽  
Assawapong Sappat ◽  
Apichai Jomphoak ◽  
Adisorn Tuantranont ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Arsenazo Iii ◽  
Passive Micromixer

Development of an optical fibre reflectance sensor for lead detection based on immobilised arsenazo III

2010 ◽  
Vol 147 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Zeynep Yanaz ◽  
Hayati Filik ◽  
Reşat Apak
Keyword(s):  
Optical Fibre ◽  
Arsenazo Iii ◽  
Lead Detection

Fluorescence and differential light absorption recordings with calcium probes and potential-sensitive dyes in skinned cardiac cells

1982 ◽  
Vol 60 (4) ◽  
pp. 556-567 ◽  
Author(s):  
Alexandre Fabiato
Keyword(s):  
Cardiac Cells ◽  
Arsenazo Iii ◽  
Optical Methods ◽  
Cardiac Cell ◽  
Differential Absorption ◽  
Fluorescence Measurements ◽  
Merocyanine 540 ◽  
Inverted Microscope ◽  
Optimum Wavelength ◽  
Absorption Measurements

This report describes an optical system for microspectrophotometry in a single cardiac cell from which the sarcolemma has been removed by microdissection (skinned cardiac cell). This system is attached to the high power inverted microscope used for the microdissection and includes (a) a single variable wavelength microspectrophotometer used to define the spectrum of a given dye or Ca2+ probe; and (b) a dual wavelength, differential microspectrophotometer used to record differentially between the optimum wavelength and a wavelength separated by 25–30 nm. Results are presented using the following optical methods: (a) fluorescence measurements with chlorotetracycline to monitor the amount of Ca2+ bound to the inner face of the sarcoplasmic reticulum (SR) membrane; (b) differential absorption measurements with arsenazo III to measure changes of myoplasmic [Ca2+]free resulting from Ca2+ release from the SR; (c) fluorescence and (or) differential absorption measurements with the potential-sensitive dyes merocyanine 540, NK 2367, and di-S-C3(5) to monitor changes of charge distribution on the SR membrane during Ca2+ accumulation in the SR, as well as before and during Ca2+-induced release of Ca2+ from the SR. A small and rapid signal is observed which precedes the Ca2+-induced release of Ca2+ from the SR. It is detected as an increase of Ca2+ binding inside the SR with chlorotetracycline and as a "hyperpolarization" with potential-sensitive dyes, while no transient change of myoplasmic [Ca2+]free is detected with arsenazo III. This small and rapid signal preceding the Ca2+ release may be a first hint to an understanding of the mechanism whereby a small increase of [Ca2+]free outside the SR triggers Ca2+ release from the SR.

Application of arsenazo III in the preparation and characterization of an albumin-linked, gadolinium-based macromolecular magnetic resonance contrast agent

2006 ◽  
Vol 157 (2) ◽  
pp. 238-245 ◽  
Author(s):  
Tavarekere N. Nagaraja ◽  
Richard L. Croxen ◽  
Swayamprava Panda ◽  
Robert A. Knight ◽  
Kelly A. Keenan ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Contrast Agent ◽  
Arsenazo Iii ◽  
Magnetic Resonance Contrast Agent ◽  
Magnetic Resonance Contrast ◽  
Resonance Contrast

Complexation of zirconium(IV), thorium(IV) and uranium(VI) malonates with Arsenazo III and Arsenazo M on fiber ion exchangers

2012 ◽  
Vol 67 (6) ◽  
pp. 527-530 ◽  
Author(s):  
V. P. Dedkova ◽  
O. P. Shvoeva ◽  
S. B. Savvin
Keyword(s):  
Arsenazo Iii ◽  
Ion Exchangers

ChemInform Abstract: KOMPLEXBLDG. VON URANYL- UND LANTHANIONEN MIT ARSENAZO III IN PERCHLORSAUREN LOESUNGEN

1970 ◽  
Vol 1 (25) ◽  
pp. no-no
Author(s):  
A. E. KLYGIN ◽  
D. M. ZAVRAZNOVA ◽  
N. S. KOLJADA
Keyword(s):  
Arsenazo Iii

scholarly journals A heart mitochondrial Ca2+-dependent pore of possible relevance to re-perfusion-induced injury. Evidence that ADP facilitates pore interconversion between the closed and open states

1990 ◽  
Vol 266 (1) ◽  
pp. 33-39 ◽  
Author(s):  
M Crompton ◽  
A Costi
Keyword(s):  
Arsenazo Iii ◽  
Heart Mitochondria ◽  
Ionophore A23187 ◽  
Relative Permeabilities ◽  
Pulsed Flow ◽  
Pore Closure ◽  
Open States ◽  
Matrix Free ◽  
Pore Number

The permeability properties of a putative Ca2(+)-activated pore in heart mitochondria, of possible relevance to re-perfusion-induced injury, have been investigated by a pulsed-flow solute-entrapment technique. The relative permeabilities of [14C]mannitol, [14C]sucrose and arsenazo III are consistent with permeation via a pore of about 2.3 nm diameter. Ca2+ removal with EGTA induced pore closure, and the mitochondria became ‘resealed’. The permeability of the unresealed mitochondria during resealing was markedly stimulated by 200 microM-ADP, and the relative permeabilities to solutes of different size were stimulated equally, indicating an increase in open-pore number, rather than an increase in pore dimensions. This is paradoxical, since ADP also stimulated the rate of resealing. The rate of EGTA-induced resealing was also stimulated by the Ca2+ ionophore A23187, which indicates that the rate of removal of matrix free Ca2+ is limiting for pore closure. An explanation for the paradox is suggested in which ADP facilitates pore interconversion between the closed and open states in permeabilized mitochondria, and pore closure in Ca2(+)-free mitochondria occurs much faster than previously thought.

scholarly journals The effects of Mg2+ and adenine nucleotides on the sensitivity of the heart mitochondrial Na+-Ca2+ carrier to extramitochondrial Ca2+. A study using arsenazo III-loaded mitochondria

1987 ◽  
Vol 244 (3) ◽  
pp. 533-538 ◽  
Author(s):  
L H Hayat ◽  
M Crompton
Keyword(s):  
Adenine Nucleotides ◽  
Mitochondrial Protein ◽  
Arsenazo Iii ◽  
Heart Mitochondria ◽  
Small Decrease ◽  
Regulatory Site ◽  
Normal Heart ◽  
Partial Inhibition ◽  
Regulatory Sites

The technique of reversible Ca2+-induced permeabilization [Al Nasser & Crompton (1986) Biochem. J. 239, 19-29, 31-40] has been applied to the preparation of heart mitochondria loaded with the Ca2+ indicator arsenazo III (2 nmol of arsenazo III/mg of mitochondrial protein). The loaded mitochondria (‘mitosomes’) were used to study the control of the Na+-Ca2+ carrier by extramitochondrial Ca2+ mediated by putative regulatory sites. The Vmax. of the Na+-Ca2+ carrier and the degree of regulatory-site-mediated inhibition were similar to normal heart mitochondria. Ca2+ occupation of the sites in mitosomes yields partial inhibition, which is half-maximal with 0.8 microM external free Ca2+. The inhibition consists of a small decrease in Vmax. and a relatively large increase in apparent Km for internal Ca2+. Mg2+ also appears to interact with the sites, but this is largely abolished by ATP and ADP (but not AMP) under conditions in which the free [Mg2+] is maintained constant. The results indicate that the regulatory sites are effective in controlling the Na+-Ca2+ carrier at physiological concentrations of adenine nucleotides, Mg2+, intra- and extra-mitochondrial free Ca2+.

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