merocyanine 540
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Author(s):  
Róisín Ann Griffin ◽  
Aleona Swegen ◽  
Mark A Baker ◽  
Rachel Ann Ogle ◽  
Nathan Smith ◽  
...  

Abstract Stallions experience transient fluctuations in fertility throughout the breeding season. Considering pregnancy diagnoses cannot be ascertained until ~14 days post-breeding, the timely detection of decreases in stallion fertility would enhance industry economic and welfare outcomes. Therefore, this study aimed to identify the proteomic signatures reflective of short-term fertility fluctuations, and to determine the biological mechanisms governing such differences. Using LC–MS/MS, we compared the proteomic profile of semen samples collected from commercially “fertile” stallions, during high- and low-fertility periods. A total of 1702 proteins were identified, of which, 38 showed a significant change in abundance (p ≤ 0.05). Assessment of intra- and inter-stallion variability revealed that caseins (namely κ-, α-S1-, and α-S2-casein), were significantly more abundant during “high-fertility” periods, while several epididymal, and seminal plasma proteins (chiefly, epididymal sperm binding protein 1 [ELSPbP1], horse seminal plasma protein 1 [HSP-1] and clusterin), were significantly more abundant during “low-fertility” periods. We hypothesised that an increased abundance of caseins offers greater protection from potentially harmful seminal plasma proteins, thereby preserving cell functionality and fertility. In vitro exposure of spermatozoa to casein resulted in decreased levels of lipid scrambling (Merocyanine 540), higher abundance of sperm-bound caseins (α-S1-, α-S2-, and κ-casein), and lower abundance of sperm-bound HSP-1 (p ≤ 0.05). This study demonstrates key pathways governing short-term fertility fluctuations in the stallion, thereby providing a platform to develop robust, fertility assessment strategies into the future.


2021 ◽  
Vol 3 (5) ◽  
pp. 1375-1381
Author(s):  
Anne Nsubuga ◽  
Gabrielle A. Mandl ◽  
John A. Capobianco

Mesoporous silica-coated NaLuF4:Dy3+,Gd3+ radioluminescent nanoparticles were loaded with photosensitizers – Rose Bengal and Merocyanine 540. ABDA, DPBF, NaN3 and EPR assays were used to determine that a type I mechanism was prevalent for this X-PDT nanosystem.


2019 ◽  
Vol 116 (3) ◽  
pp. 82a
Author(s):  
Rupam Dutta ◽  
Arghajit Pyne ◽  
Sangita Kundu ◽  
Pavel Banerjee ◽  
Nilmoni Sarkar
Keyword(s):  

2018 ◽  
Vol 83 ◽  
pp. 165-175 ◽  
Author(s):  
Uzma Jabeen ◽  
Tham Adhikari ◽  
Syed Mujtaba Shah ◽  
Sajid Ullah Khan ◽  
Dinesh Pathak ◽  
...  

2018 ◽  
Vol 64 (5) ◽  
pp. 445-449 ◽  
Author(s):  
Lauro GONZÁLEZ-FERNÁNDEZ ◽  
Beatriz MACÍAS-GARCÍA ◽  
Violeta CALLE-GUISADO ◽  
Luis Jesús GARCÍA-MARÍN ◽  
María Julia BRAGADO

Langmuir ◽  
2017 ◽  
Vol 33 (49) ◽  
pp. 14209-14209 ◽  
Author(s):  
Rupam Dutta ◽  
Arghajit Pyne ◽  
Sangita Kundu ◽  
Pavel Banerjee ◽  
Nilmoni Sarkar
Keyword(s):  

Langmuir ◽  
2017 ◽  
Vol 33 (38) ◽  
pp. 9811-9821 ◽  
Author(s):  
Rupam Dutta ◽  
Arghajit Pyne ◽  
Sangita Kundu ◽  
Pavel Banerjee ◽  
Nilmoni Sarkar
Keyword(s):  

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