The Seed-specific Oleosin Promoter of Brassica juncea: Cloning, Characterization and Identification of the Novel and Divergent Antisense Open Reading Frame

Hepatitis C virus contains a second open reading frame within the core gene, designated core+1/ARF. Here we demonstrate for the first time expression of core+1/ARF protein in the context of a bicistronic JFH1-based replicon and report the production of two isoforms, core+1/L (long) and core+1/S (short), with different kinetics.

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pDP4, a novel glycoprotein secreted by mature granulocytes, is regulated by transcription factor PU.1

Blood ◽

10.1182/blood-2003-08-2688 ◽

2004 ◽

Vol 103 (11) ◽

pp. 4294-4301 ◽

Cited By ~ 24

Author(s):

Frank Rosenbauer ◽

Katharina Wagner ◽

Pu Zhang ◽

Klaus-Peter Knobeloch ◽

Atsushi Iwama ◽

...

Keyword(s):

Transcription Factor ◽

Open Reading Frame ◽

The Novel ◽

Chromosome 14 ◽

Reading Frame ◽

Lymphoid Development ◽

C Terminus ◽

Extracellular Glycoprotein ◽

Deficient Mice

Abstract The transcription factor PU.1 (Spi-1) is a well-characterized regulator of myeloid and lymphoid development. However, its role in mature functional cells is poorly studied. Here we report the characterization of the novel murine gene pDP4 (PU.1 difference product 4), which is absent from fetal livers of PU.1-deficient mice. pDP4 is transcribed as a single 3.2-kb mRNA with a 1518-base pair open reading frame encoded by 5 exons on chromosome 14. pDP4 expression is strongest in small intestine and bone marrow, in which it is expressed predominately in mature neutrophils. Interestingly, however, pDP4 expression is markedly down-regulated in neutrophils of the peripheral blood and peritoneum. The pDP4 gene encodes a secreted 57-kDa glycoprotein with an olfactomedin-like C-terminus. PU.1 binds to a functional site within the pDP4 promoter, and pDP4 expression in myeloid cells is strictly dependent on PU.1 and the presence of this site. In conclusion, we have identified a novel PU.1-regulated extracellular glycoprotein of the olfactomedin-like family with a possible role in neutrophilic trafficking. (Blood. 2004;103:4294-4301)

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Chicken Astrovirus (CAstV) Molecular Studies Reveal Evidence of Multiple Past Recombination Events in Sequences Originated from Clinical Samples of White Chick Syndrome (WCS) in Western Canada

Viruses ◽

10.3390/v12101096 ◽

2020 ◽

Vol 12 (10) ◽

pp. 1096

Author(s):

Victor Palomino-Tapia ◽

Darko Mitevski ◽

Tom Inglis ◽

Frank van der Meer ◽

Emily Martin ◽

...

Keyword(s):

Phylogenetic Analyses ◽

Point Mutations ◽

Open Reading Frame ◽

Clinical Samples ◽

Western Canada ◽

The Novel ◽

Genome Sequences ◽

Sequence Comparisons ◽

Reading Frame ◽

Detection Software

In this study, we aimed to molecularly characterize 14 whole genome sequences of chicken astrovirus (CAstV) isolated from samples obtained from white chick syndrome (WCS) outbreaks in Western Canada during the period of 2014–2019. Genome sequence comparisons showed all these sequences correspond to the novel Biv group from which no confirmed representatives were published in GenBank. Molecular recombination analyses using recombination detection software (i.e., RDP5 and SimPlot) and phylogenetic analyses suggest multiple past recombination events in open reading frame (ORF)1a, ORF1b, and ORF2. Our findings suggest that recombination events and the accumulation of point mutations may have contributed to the substantial genetic variation observed in CAstV and evidenced by the current seven antigenic sub-clusters hitherto described. This is the first paper that describes recombination events in CAstV following analysis of complete CAstV sequences originated in Canada.

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