Studies on the solubilization of basic polypeptides in reversed micelles by ultracentrifuge measurements

Author(s):  
G. Ebert ◽  
M. Plachky ◽  
M. Seno ◽  
S. Shoji

1985 ◽  
Vol 50 (12) ◽  
pp. 2925-2936 ◽  
Author(s):  
Štěpánka Štokrová ◽  
Jan Pospíšek ◽  
Jaroslav Šponar ◽  
Karel Bláha

Polypeptides (Lys-X-Ala)n and (Lys-X-Gly)n in which X represents residues of isoleucine and norleucine, respectively, and polypeptide (Tle-Lys-Ala)n, were synthesized via polymerization of 1-hydroxysuccinimidyl esters of the appropriate tripeptides to complete previously studied series. Circular dichroism (CD) spectra of the respective polymers were measured as a function of pH and salt concentration of the medium. The results were correlated with those obtained previously with the same series containing different amino acid residues at the X-position. The helix forming ability of the polypeptides (Lys-X-Ala)n with linear X side chain was found to be independent of the length. In the series (Lys-X-Gly)n the unordered conformation was the most probable one except (Lys-Ile-Gly)n. This polymer assumed the β conformation even in low salt solution at neutral pH. An agreement with some theoretical work concerned with the restriction of conformational freedom of amino acid residue branching at Cβ atom with our experimental results is evident.



1979 ◽  
Vol 254 (12) ◽  
pp. 5049-5052 ◽  
Author(s):  
M Yamamoto ◽  
W E Criss ◽  
Y Takai ◽  
H Yamamura ◽  
Y Nishizuka


1990 ◽  
Vol 187 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Bozena TYRAKOWSKA ◽  
Raymond M. D. VERHAERT ◽  
Riet HILHORST ◽  
Cees VEEGER


1989 ◽  
Vol 62 (9) ◽  
pp. 2854-2861 ◽  
Author(s):  
Ayako Goto ◽  
Hiroshi Kishimoto


Langmuir ◽  
2003 ◽  
Vol 19 (15) ◽  
pp. 5984-5990 ◽  
Author(s):  
Helga Füredi-Milhofer ◽  
Alexey Kamishny ◽  
Junko Yano ◽  
Abraham Aserin ◽  
Nissim Garti


1986 ◽  
Vol 33 (2) ◽  
pp. B27-B33 ◽  
Author(s):  
M. Dekker ◽  
K. Van 't Triet ◽  
S.R. Weijers ◽  
J.W.A. Baltussen ◽  
C. Laane ◽  
...  


1990 ◽  
Vol 1036 (1) ◽  
pp. 34-40 ◽  
Author(s):  
Anil K. Singh ◽  
Camille Sandorfy ◽  
Janos H. Fendler




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