Release of Outer Membrane Vesicles from Bordetella pertussis

1999 ◽  
Vol 38 (5) ◽  
pp. 273-278 ◽  
Author(s):  
D. Hozbor ◽  
M.E. Rodriguez ◽  
J. Fernández ◽  
A. Lagares ◽  
N. Guiso ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Bordetella Pertussis ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles

scholarly journals Canonical and Non-canonical Inflammasome Activation by Outer Membrane Vesicles Derived From Bordetella pertussis

2020 ◽  
Vol 11 ◽  
Author(s):  
Maia L. Elizagaray ◽  
Marco Túlio R. Gomes ◽  
Erika S. Guimaraes ◽  
Martín Rumbo ◽  
Daniela F. Hozbor ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Bordetella Pertussis ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Inflammasome Activation

scholarly journals A streamlined method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis

2018 ◽  
Vol 5 (2) ◽  
pp. 43-46
Author(s):  
M Sekhavati ◽  
SD Siadat ◽  
M Noofeli ◽  
A Mohebati Mobarez ◽  
◽  
...  
Keyword(s):  
Outer Membrane ◽  
Bordetella Pertussis ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles

Outer membrane vesicles obtained from Bordetella pertussis Tohama expressing the lipid A deacylase PagL as a novel acellular vaccine candidate

Vaccine ◽  
2011 ◽  
Vol 29 (8) ◽  
pp. 1649-1656 ◽  
Author(s):  
Cristian J.A. Asensio ◽  
María Emilia Gaillard ◽  
Griselda Moreno ◽  
Daniela Bottero ◽  
Eugenia Zurita ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Bordetella Pertussis ◽  
Lipid A ◽  
Vaccine Candidate ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Acellular Vaccine

scholarly journals Extraction of Outer membrane Vesicles from Vaccinal Strain of Bordetella Pertussis as the First Step of a Vaccine Candidate Study Against Pertussis Infection

2020 ◽  
Vol 14 (3) ◽  
pp. 213-226
Author(s):  
Maryam Sadat Soltani ◽  
Fereshteh Eftekhar ◽  
Fereshteh Shahcheraghi ◽  
Mojtaba Noofeli ◽  
Seyed Reza Banihashemi ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Bordetella Pertussis ◽  
Vaccine Candidate ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Pertussis Infection ◽  
Vaccinal Strain

A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain

2020 ◽  
Author(s):  
Mohammad Sekhavati ◽  
Ashraf Mohabati Mobarez ◽  
Seyed Davar Siadat ◽  
Mojtaba Noofeli
Keyword(s):  
Outer Membrane ◽  
Bordetella Pertussis ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Elisa Assay ◽  
Transmission Electron ◽  
Sds Page ◽  
Novel Method ◽  
Centrifugation Technique ◽  
New Generation

Background and Objectives: There are many pertussis outbreaks which is mainly due to the reduction in the immunity of acellular pertussis (aP) vaccines. Therefore, there is a crucial necessity to develop a new generation of pertussis vaccine. Pre- ceding researches have shown that Bordetella pertussis outer membrane vesicles (OMVs) have appropriate specifications, making them a suitable vaccine candidate against pertussis. Materials and Methods: The OMVs were separated by a new serial ultra centrifugation technique. Transmission electron microscopy (TEM) examination, SDS-PAGE, Western blotting and ELISA assay were used to characterize the OMVs. Results: TEM studies showed the size of the extracted OMVs at 40-200 nm. The presence of pertussis toxin, filamentous hemagglutinin, and pertactin was verified using Western blot and ELISA assay. Conclusion: The presented technique is a simple and effective way to obtain OMVs from Bordetella pertussis. So it can be utilized as an appropriate procedure in the development of an OMV-based vaccine against pertussis.  

scholarly journals Evaluation of Outer Membrane Vesicles Obtained from Predominant Local Isolate of Bordetella pertussis as a Vaccine Candidate

2021 ◽  
Vol 25 (6) ◽  
pp. 399-407
Author(s):  
Maryam sadat Soltani ◽  
Mojtaba Noofeli ◽  
Seyed Reza Banihashemi ◽  
Fereshteh Shahcheraghi ◽  
Fereshteh Eftekhar ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Bordetella Pertussis ◽  
Vaccine Candidate ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Local Isolate

Electrophysiological Characterization of Transport Across Outer Membrane Channels from Gram-Negative Bacteria in Their Native Environment

2019 ◽  
Author(s):  
Jiajun Wang ◽  
Rémi Terrasse ◽  
Jayesh Arun Bafna ◽  
Lorraine Benier ◽  
Mathias Winterhalter
Keyword(s):  
Outer Membrane ◽  
Lipid Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Multi Drug Resistance ◽  
Gram Negative Bacteria ◽  
Membrane Channels ◽  
Gram Negative ◽  
Electrophysiological Characterization

Multi-drug resistance in Gram-negative bacteria is often associated with low permeability of the outer membrane. To investigate the role of membrane channels in the uptake of antibiotics, we extract, purify and reconstitute them into artificial planar membranes. To avoid this time-consuming procedure, here we show a robust approach using fusion of native outer membrane vesicles (OMV) into planar lipid bilayer which moreover allows also to some extend the characterization of membrane protein channels in their native environment. Two major membrane channels from <i>Escherichia coli</i>, OmpF and OmpC, were overexpressed from the host and the corresponding OMVs were collected. Each OMV fusion revealed surprisingly single or only few channel activities. The asymmetry of the OMV´s translates after fusion into the lipid membrane with the LPS dominantly present at the side of OMV addition. Compared to conventional reconstitution methods, the channels fused from OMVs containing LPS have similar conductance but a much broader distribution. The addition of Enrofloxacin on the LPS side yields somewhat higher association (<i>k<sub>on</sub></i>) and lower dissociation (<i>k<sub>off</sub></i>) rates compared to LPS-free reconstitution. We conclude that using outer membrane vesicles is a fast and easy approach for functional and structural studies of membrane channels in the native membrane.

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