Optimal mode of operation for temperature induction of bacteriophage λQ− mutant in Escherichia coli

1998 ◽  
Vol 19 (1) ◽  
pp. 7-10
Author(s):  
B.-Y. Chen ◽  
H. C. Lim

1998 ◽  
Vol 19 (1) ◽  
pp. 7
Author(s):  
B.-Y. Chen ◽  
H. C. Lim


1998 ◽  
Vol 9 (3) ◽  
pp. 237-243
Author(s):  
V. I. Bimatov ◽  
I. A. Druzhinin ◽  
I. V. Druzhinina ◽  
E. E. Libin ◽  
S. V. Chakhlov


2002 ◽  
Vol 57 (14) ◽  
pp. 2799-2809 ◽  
Author(s):  
Ben H.L Betlem ◽  
Pieter Mulder ◽  
Brian Roffel




2021 ◽  
Vol 680 (1) ◽  
pp. 012032
Author(s):  
Yi Zhang ◽  
Feng Zhang ◽  
Youchun Li ◽  
Jianguo Mo ◽  
Lv Tang


2020 ◽  
Vol 129 (1D) ◽  
pp. 61-65
Author(s):  
Van Thanh Dang ◽  
Anh Thi Hoang ◽  
Gia Cat Tuong Tran ◽  
Thi Huyen Tran Pham ◽  
Le Thi Ha Thanh ◽  
...  

Catechol 1,2-dioxygenase (CAT1) is a key enzyme for the ortho-cleavage pathway involved in the degradation of dibenzofuran, a dioxin derivative, which is a highly toxic environmental pollutant. The present study aims to investigate appropriate culture conditions for enhancing the expression of the cat1 gene encoding CAT1 enzyme from Burkholderia cepacia DF4 in Escherichia coli M15. The optimized culture conditions for gene expression are cell density at the time of induction, shaking speed, induction temperature, induction time, and inducer concentration. The highest level for CAT1 was obtained at the IPTG concentration of 1.2 mM, 10 hours after induction at 35 °C, shaking speed 200 rpm with cell density at OD600 0.7.



2019 ◽  
Vol 180 (1) ◽  
pp. 16-20
Author(s):  
Н. Гегамян ◽  
N. S. Gegamyan ◽  
Л. Киселев ◽  
L. Kiselev ◽  
В. Киселев ◽  
...  


1995 ◽  
Vol 40 (2) ◽  
pp. 87-97 ◽  
Author(s):  
Bor-Yann Chen ◽  
Chan-Shing Lin ◽  
Henry C Lim


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