Improvement of artemisinin accumulation in hairy root cultures of Artemisia annua L by fungal elicitor

1999 ◽  
Vol 20 (2) ◽  
pp. 161-164 ◽  
Author(s):  
C. Liu ◽  
Y. Wang X. Xu ◽  
F. Ouyang ◽  
H. Ye ◽  
G. Li
2007 ◽  
Vol 29 (7) ◽  
pp. 1143-1146 ◽  
Author(s):  
Waraporn Putalun ◽  
Wanwimon Luealon ◽  
Wanchai De-Eknamkul ◽  
Hiroyuki Tanaka ◽  
Yukihiro Shoyama

1998 ◽  
Vol 19 (5) ◽  
pp. 389-392 ◽  
Author(s):  
C. Liu ◽  
Y. Wang ◽  
C. Guo ◽  
F. Ouyang ◽  
H. Ye ◽  
...  

Author(s):  
Fadjar Aju Tofiana ◽  
Immaculata Iwo M ◽  
Rahmana Emran Kartasasmita ◽  
Elfahmi .

<p>ABSTRACT<br />Objective: To find the stigmasterol source of Artemisia annua L. and to study the profile of phytosterol types in A. annua hairy root cultures.<br />Methods: Stigmasterol content determination was done based on stigmasterol content in the methylene chloride extract of A. annua using high<br />performance liquid chromatography in wild-type plant, plant tissue cultures, and hairy root cultures. The optimal resolution was achieved by<br />suitability system, and stigmasterol calibration curve was generated with stigmasterol concentrations series. Phytosterol types in A. annua hairy root<br />cultures were performed by gas chromatography-mass spectrophotometry.<br />Results: Highest stigmasterol content in A. annua is shown by hairy root cultures of A. annua in the 5<br /> week harvesting time, which is 1.5 g/100 g (w/w)<br />extract. The phytosterol profile in A. annua hairy root cultures encompassed stigmasterol 74.6%, β-sitosterol 13.5%, and campesterol 11.9%.<br />th<br />Conclusion: A. annua hairy root cultures in 5-week harvesting time prove its potential as stigmasterol source alternative. The highest phytosterol type<br />in A. annua hairy root cultures is stigmasterol. This is the first report stigmasterol content and its phytosterol profile of A. annua hairy root cultures<br />extract.<br />Keywords: Artemisia annua L., Wild-type plant, Plant tissue cultures, Hairy root cultures, Stigmasterol.</p>


RSC Advances ◽  
2020 ◽  
Vol 10 (65) ◽  
pp. 39434-39446
Author(s):  
Natalia Kobylinska ◽  
Anatolij Shakhovsky ◽  
Olena Khainakova ◽  
Dmytro Klymchuk ◽  
Liliya Avdeeva ◽  
...  

The research was focused on the synthesis of silver nanoparticles (AgNPs) using extracts from the “hairy” root cultures of Artemisia tilesii Ledeb. and Artemisia annua L.


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