Enhanced production of artemisinin by Artemisia annua L hairy root cultures in a modified inner-loop airlift bioreactor

1998 ◽  
Vol 19 (5) ◽  
pp. 389-392 ◽  
Author(s):  
C. Liu ◽  
Y. Wang ◽  
C. Guo ◽  
F. Ouyang ◽  
H. Ye ◽  
...  
Keyword(s):  
Hairy Root ◽  
Artemisia Annua ◽  
Airlift Bioreactor ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Artemisia Annua L ◽  
Enhanced Production

Improvement of artemisinin production by chitosan in hairy root cultures of Artemisia annua L.

2007 ◽  
Vol 29 (7) ◽  
pp. 1143-1146 ◽  
Author(s):  
Waraporn Putalun ◽  
Wanwimon Luealon ◽  
Wanchai De-Eknamkul ◽  
Hiroyuki Tanaka ◽  
Yukihiro Shoyama
Keyword(s):  
Hairy Root ◽  
Artemisia Annua ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Artemisia Annua L

scholarly journals STIGMASTEROL CONTENT OF ARTEMISIA ANNUA L. AND THE PHYTOSTEROL PROFILE

2016 ◽  
pp. 239
Author(s):  
Fadjar Aju Tofiana ◽  
Immaculata Iwo M ◽  
Rahmana Emran Kartasasmita ◽  
Elfahmi .
Keyword(s):  
Hairy Root ◽  
Artemisia Annua ◽  
Wild Type Plant ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Tissue Cultures ◽  
Wild Type ◽  
Harvesting Time ◽  
Artemisia Annua L ◽  
Plant Tissue Cultures

<p>ABSTRACT<br />Objective: To find the stigmasterol source of Artemisia annua L. and to study the profile of phytosterol types in A. annua hairy root cultures.<br />Methods: Stigmasterol content determination was done based on stigmasterol content in the methylene chloride extract of A. annua using high<br />performance liquid chromatography in wild-type plant, plant tissue cultures, and hairy root cultures. The optimal resolution was achieved by<br />suitability system, and stigmasterol calibration curve was generated with stigmasterol concentrations series. Phytosterol types in A. annua hairy root<br />cultures were performed by gas chromatography-mass spectrophotometry.<br />Results: Highest stigmasterol content in A. annua is shown by hairy root cultures of A. annua in the 5<br /> week harvesting time, which is 1.5 g/100 g (w/w)<br />extract. The phytosterol profile in A. annua hairy root cultures encompassed stigmasterol 74.6%, β-sitosterol 13.5%, and campesterol 11.9%.<br />th<br />Conclusion: A. annua hairy root cultures in 5-week harvesting time prove its potential as stigmasterol source alternative. The highest phytosterol type<br />in A. annua hairy root cultures is stigmasterol. This is the first report stigmasterol content and its phytosterol profile of A. annua hairy root cultures<br />extract.<br />Keywords: Artemisia annua L., Wild-type plant, Plant tissue cultures, Hairy root cultures, Stigmasterol.</p>

Improvement of artemisinin accumulation in hairy root cultures of Artemisia annua L by fungal elicitor

1999 ◽  
Vol 20 (2) ◽  
pp. 161-164 ◽  
Author(s):  
C. Liu ◽  
Y. Wang X. Xu ◽  
F. Ouyang ◽  
H. Ye ◽  
G. Li
Keyword(s):  
Hairy Root ◽  
Artemisia Annua ◽  
Fungal Elicitor ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Artemisia Annua L

Enhanced production of falcarinol-type polyacetylenes in hairy roots of cultivated carrot (Daucus carota subsp. sativus)

2021 ◽  
Author(s):  
Frank Dunemann ◽  
Christoph Böttcher
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Higher Plants ◽  
Leaf Tissue ◽  
Hairy Root Cultures ◽  
Scale Production ◽  
Root Cultures ◽  
Tissue Samples ◽  
Positive Effects ◽  
Enhanced Production

Abstract Polyacetylenes (PAs) are a large group of bioactive phytochemicals, which are primarily produced by higher plants of the families Apiaceae and Araliaceae. Especially aliphatic C17-polyacetylenes of the falcarinol-type such as falcarinol (FaOH) and falcarindiol (FaDOH) are known for their numerous positive effects on human health. In this study we investigate the potential of carrot hairy root cultures for production of PAs. Three individual plants of seven differently coloured carrot cultivars were used for the development of hairy root cultures by transformation of root discs with the wild-type Rhizobium rhizogenes strain 15834. A total of 51 individual hairy root (HR) lines were obtained and quantitatively analysed together with root, petiole and leaf tissue samples for FaOH and FaDOH. Among the five tissues sampled from the donor plants, root periderm samples generally exhibited the highest PA levels with FaDOH as prevailing PA and large differences between cultivars. In comparison to periderm tissue, FaOH levels were highly increased in HR lines of all cultivars. In contrast, FaDOH levels were not significantly altered. Considering the low to moderate PA concentration in root and leaf tissues of the orange cultivars there was an up to more than 10-fold increase of the FaOH concentration in HRs of these genotypes. Within this study a reproducible method for Rrhi-mediated transformation of carrot root discs was applied which provides an efficient tool to assess the function of candidate genes involved in the biosynthesis of key PAs in carrot but might be used in future also for the large-scale production of falcarinol-type PAs.

Enhanced production of valepotriates by Agrobacterium rhizogenes induced hairy root cultures of Valeriana wallichii DC

Plant Science ◽  
1998 ◽  
Vol 131 (2) ◽  
pp. 203-208 ◽  
Author(s):  
Suchitra Banerjee ◽  
L Rahman ◽  
G.C Uniyal ◽  
P.S Ahuja
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Enhanced Production

Enhanced production of the alkaloid nicotine in hairy root cultures of Nicotiana tabacum L.

2012 ◽  
Vol 113 (1) ◽  
pp. 121-129 ◽  
Author(s):  
Bo Zhao ◽  
Foster A. Agblevor ◽  
Ritesh K. C. ◽  
John G. Jelesko
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Enhanced Production ◽  
Nicotiana Tabacum L
Sign in / Sign up

Export Citation Format

Share Document