Chicken type II collagen induced immune balance of main subtype of helper T cells in mesenteric lymph node lymphocytes in rats with collagen-induced arthritis

2009 ◽  
Vol 59 (5) ◽  
pp. 369-377 ◽  
Author(s):  
Tong Tong ◽  
Wei Zhao ◽  
Ying-Qi Wu ◽  
Yan Chang ◽  
Qing-Tong Wang ◽  
...  
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Type Ii Collagen ◽  
Helper T Cells ◽  
Type Ii ◽  
Collagen Induced Arthritis ◽  
Mesenteric Lymph ◽  
Immune Balance

scholarly journals Dexamethasone Preconditioning Improves the Response of Collagen-Induced Arthritis to Treatment with Short-Term Lipopolysaccharide-Stimulated Collagen-Loaded Dendritic Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Corina Peña ◽  
David Gárate ◽  
Juan Contreras-Levicoy ◽  
Octavio Aravena ◽  
Diego Catalán ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Cd4 T Cells ◽  
Combined Treatment ◽  
Type Ii Collagen ◽  
Cytokine Profile ◽  
Clinical Disease ◽  
Type Ii ◽  
Collagen Induced Arthritis ◽  
Short Term

Background. Pharmacologically modulated dendritic cells (DCs) have been shown to restore tolerance in type II collagen-(CII-) induced arthritis (CIA). We examined the effect of dexamethasone (DXM) administration as a preconditioning agent, followed by an injection of lipopolysaccharide-(LPS-) stimulated and CII-loaded DCs on the CIA course.Methods. After CIA induction, mice pretreated with DXM were injected with 4-hour LPS-stimulated DCs loaded with CII (DXM/4hLPS/CII/DCs).Results. Mice injected with DXM/4hLPS/CII/DCs displayed significantly less severe clinical disease compared to animals receiving 4hLPS/CII/DCs alone or those in which only DXM was administered. Cytokine profile evaluation showed that CD4+ T cells from DXM/4hLPS/CII/DCs and 4hLPS/CII/DCs groups release higher IL-10 levels than those from mice receiving DXM alone or CIA mice. CD4+ T cells from all DC-treated groups showed less IL-17 release when compared to the CIA group. On the contrary, CD4+ T cells from DXM/4hLPS/CII/DCs and 4hLPS/CII/DCs groups released higher IFN-γlevels than those from CIA group.Conclusion. A combined treatment, including DXM preconditioning followed by an inoculation of short-term LPS-stimulated CII-loaded DCs, provides an improved strategy for attenuating CIA severity. Our results suggest that this benefit is driven by a modulation in the cytokine profile secreted by CD4+ T cells.

scholarly journals Mesenteric lymph node stromal cell-derived extracellular vesicles contribute to peripheral de novo induction of Foxp3+ regulatory T cells

2017 ◽  
Vol 47 (12) ◽  
pp. 2142-2152 ◽  
Author(s):  
Maria Pasztoi ◽  
Joern Pezoldt ◽  
Michael Beckstette ◽  
Christoph Lipps ◽  
Dagmar Wirth ◽  
...  
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
Regulatory T Cells ◽  
Extracellular Vesicles ◽  
Mesenteric Lymph Node ◽  
Stromal Cell ◽  
De Novo ◽  
Mesenteric Lymph

Effects of Pentoxyfylline on Mesenteric Lymph Node T-Cells in a Rat Model of Thermal Injury

Shock ◽  
2003 ◽  
Vol 20 (6) ◽  
pp. 517-520 ◽  
Author(s):  
Bharat K. Kotadia ◽  
Thyyar M. Ravindranath ◽  
Mashkoor A. Choudhry ◽  
Farah Haque ◽  
Walid Al-Ghoul ◽  
...  
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
Rat Model ◽  
Mesenteric Lymph Node ◽  
Thermal Injury ◽  
Mesenteric Lymph

Abnormal development of Hymenolepis nana larvae in immunosuppressed mice

1980 ◽  
Vol 54 (2) ◽  
pp. 75-82 ◽  
Author(s):  
S. B. Lucas ◽  
O. Hassounah ◽  
R. Muller ◽  
M. J. Doenhoff
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Natural Infection ◽  
Human Infection ◽  
Abnormal Development ◽  
Hydrocortisone Acetate ◽  
Hymenolepis Nana ◽  
Mesenteric Lymph ◽  
Immunosuppressed Mice

ABSTRACTObservations on the course of Hymenolepis nana infection in immunosuppressed mice are presented. Treatment of the host with hydrocortisone acetate caused superinfection of the bowel with worms and the development of normal cysticercoids in the mesenteric lymph node and liver. Natural infection of mice deprived of their T-cells by pre-adult thymectomy and administration of antithymocyte serum resulted in superinfection and widespread metastasis of aberrant cysticercoids that were greatly enlarged and without scolices, causing death after about five months. The significance of these findings and their possible relevance to human infection with H. nana are discussed.

scholarly journals Crucial roles of B7-H1 and B7-DC expressed on mesenteric lymph node dendritic cells in the generation of antigen-specific CD4+Foxp3+ regulatory T cells in the establishment of oral tolerance

Blood ◽  
2010 ◽  
Vol 116 (13) ◽  
pp. 2266-2276 ◽  
Author(s):  
Tomohiro Fukaya ◽  
Hideaki Takagi ◽  
Yumiko Sato ◽  
Kaori Sato ◽  
Kawori Eizumi ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Lymph Node ◽  
Regulatory T Cells ◽  
Mesenteric Lymph Node ◽  
Oral Tolerance ◽  
Family Members ◽  
T Cell Response ◽  
Mesenteric Lymph ◽  
B7 Family

Abstract Oral tolerance is a key feature of intestinal immunity, generating systemic tolerance to fed antigens. However, the molecular mechanism mediating oral tolerance remains unclear. In this study, we examined the role of the B7 family members of costimulatory molecules in the establishment of oral tolerance. Deficiencies of B7-H1 and B7-DC abrogated the oral tolerance, accompanied by enhanced antigen-specific CD4+ T-cell response and IgG1 production. Mesenteric lymph node (MLN) dendritic cells (DCs) displayed higher levels of B7-H1 and B7-DC than systemic DCs, whereas they showed similar levels of CD80, CD86, and B7-H2. MLN DCs enhanced the antigen-specific generation of CD4+Foxp3+ inducible regulatory T cells (iTregs) from CD4+Foxp3− T cells rather than CD4+ effector T cells (Teff) relative to systemic DCs, owing to the dominant expression of B7-H1 and B7-DC. Furthermore, the antigen-specific conversion of CD4+Foxp3− T cells into CD4+Foxp3+ iTregs occurred in MLNs greater than in peripheral organs during oral tolerance under steady-state conditions, and such conversion required B7-H1 and B7-DC more than other B7 family members, whereas it was severely impaired under inflammatory conditions. In conclusion, our findings suggest that B7-H1 and B7-DC expressed on MLN DCs are essential for establishing oral tolerance through the de novo generation of antigen-specific CD4+Foxp3+ iTregs.

scholarly journals Stepwise chromatin and transcriptional acquisition of an intraepithelial lymphocyte program

2020 ◽  
Author(s):  
Mariya London ◽  
Angelina M. Bilate ◽  
Tiago B. R. Castro ◽  
Daniel Mucida
Keyword(s):  
T Cells ◽  
Transcription Factor ◽  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Trajectory Analysis ◽  
Mouse Genetics ◽  
Site Specific ◽  
Intestinal Lamina Propria ◽  
Mesenteric Lymph ◽  
Gut Epithelium

AbstractMesenteric lymph node (mLN) T cells undergo tissue adaptation upon migrating to intestinal lamina propria (LP) and intraepithelial (IE) compartments, ensuring appropriate balance between tolerance and resistance. By combining mouse genetics with single-cell and chromatin analyses, we addressed the molecular imprinting of gut epithelium on T cells. Transcriptionally, conventional and regulatory (Treg) CD4+ T cells from mLN, LP and IE segregate based on the gut layer they occupy; trajectory analysis suggests a stepwise loss of CD4-programming and acquisition of an intraepithelial profile. Treg fate–mapping coupled with RNA– and ATAC–sequencing revealed that the Treg program shuts down before an intraepithelial program becomes fully accessible at the epithelium. Ablation of CD4 lineage–defining transcription factor ThPOK results in premature acquisition of an IEL profile by mLN Tregs, partially recapitulating epithelium imprinting. Thus, coordinated replacement of circulating lymphocyte program with site–specific transcriptional and chromatin changes is necessary for tissue imprinting.

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