The negative feedback regulation of microRNA-146a in human periodontal ligament cells after Porphyromonas gingivalis lipopolysaccharide stimulation

2015 ◽  
Vol 64 (6) ◽  
pp. 441-451 ◽  
Author(s):  
Shao-Yun Jiang ◽  
Dong Xue ◽  
Yu-Feng Xie ◽  
Dong-Wang Zhu ◽  
Yun-Yun Dong ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Negative Feedback ◽  
Periodontal Ligament ◽  
Feedback Regulation ◽  
Periodontal Ligament Cells ◽  
Negative Feedback Regulation ◽  
Human Periodontal Ligament Cells ◽  
Porphyromonas Gingivalis Lipopolysaccharide

scholarly journals Methanandamide diminish the Porphyromonas gingivalis lipopolysaccharide induced response in human periodontal ligament cells

2020 ◽  
Author(s):  
Fengqiu Zhang ◽  
Burcu Özdemir ◽  
Phuong Quynh Nguyen ◽  
Oleh Andrukhov ◽  
Xiaohui Rausch-Fan
Keyword(s):  
Porphyromonas Gingivalis ◽  
Periodontal Ligament ◽  
Cannabinoid Receptors ◽  
Endocannabinoid System ◽  
Periodontal Ligament Cells ◽  
Induced Response ◽  
Human Periodontal Ligament Cells ◽  
Chemotactic Protein ◽  
Improved Stability ◽  
Porphyromonas Gingivalis Lipopolysaccharide

Abstract Background: The endocannabinoid system is involved in the regulation of periodontal tissue homeostasis. Synthetic cannabinoid methanandamide (Meth-AEA) has improved stability and affinity to cannabinoid receptors compared to its endogenous analog anandamide. In the present study, we investigated the effect of methanandamide on the production of pro-inflammatory mediators in primary human periodontal ligament cells (hPdLCs).Methods: hPdLCs were treated with Meth-AEA for 24 h, and the resulting production of interleukin (IL)-6, IL-8, and monocyte chemotactic protein (MCP)-1 was measured in the absence or the presence of Porphyromonas gingivalis lipopolysaccharide (LPS). Additionally, the effect of Meth-AEA on the proliferation/viability of hPdLCs was measured by MTT method. Results: Methanandamide at a concentration of 10 µM significantly inhibited P. gingivalis LPS induced production of IL-6, IL-8, and MCP-1. Basal production of IL-6 and IL-8 was slightly enhanced by 10 µM Meth-AEA. No effect of Meth-AEA on the basal production of MCP-1 was observed. Meth-AEA in concentrations up to 10 µM did not affect the proliferation of hPdLCs, but significantly inhibited it at a concentration of 30 µM. Conclusion: Our study suggests that the inflammatory response in periodontal ligament cells could be influenced by the activation of the cannabinoid system, which might be potentially involved in the progression of periodontal disease.

Activation of MMP-2 by Porphyromonas gingivalis in human periodontal ligament cells

2003 ◽  
Vol 38 (2) ◽  
pp. 115-121 ◽  
Author(s):  
Kassara Pattamapun ◽  
Siriluck Tiranathanagul ◽  
Tussanee Yongchaitrakul ◽  
Jintakorn Kuwatanasuchat ◽  
Prasit Pavasant
Keyword(s):  
Porphyromonas Gingivalis ◽  
Periodontal Ligament ◽  
Periodontal Ligament Cells ◽  
Human Periodontal Ligament Cells

Cytokine production in human periodontal ligament cells stimulated with Porphyromonas gingivalis

2006 ◽  
Vol 41 (6) ◽  
pp. 554-559 ◽  
Author(s):  
T. Yamamoto ◽  
M. Kita ◽  
F. Oseko ◽  
T. Nakamura ◽  
J. Imanishi ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Periodontal Ligament ◽  
Cytokine Production ◽  
Periodontal Ligament Cells ◽  
Human Periodontal Ligament Cells

scholarly journals Effects of methanandamide on human periodontal ligament cells

2019 ◽  
Author(s):  
Fengqiu Zhang ◽  
Burcu Özdemir ◽  
Phuong Quynh Nguyen ◽  
Oleh Andrukhov ◽  
Xiaohui Rausch-Fan
Keyword(s):  
Periodontal Ligament ◽  
Cannabinoid Receptors ◽  
Endocannabinoid System ◽  
Periodontal Ligament Cells ◽  
Periodontal Tissue ◽  
Inflammatory Conditions ◽  
Human Periodontal Ligament Cells ◽  
Chemotactic Protein ◽  
Improved Stability ◽  
Porphyromonas Gingivalis Lipopolysaccharide

Abstract Background Endocannabinoid system is involved in the regulation of periodontal tissue homeostasis. Synthetic cannabinoid methanandamide (Meth-AEA) has an improved stability and affinity to cannabinoid receptors compared to its endogenous analogue anandamide. In the present study, we investigated the effect of methanandamide on the production of pro-inflammatory mediators in primary human periodontal ligament cells (hPdLCs). Methods hPdLCs were treated with Meth-AEA for 24 h and resulting production of interleukin (IL)-6, IL-8, and monocyte chemotactic protein (MCP)-1 was measured under normal condition as well as under inflammatory conditions mimicked by the presence of Porphyromonas gingivalis lipopolysaccharide (LPS). Additionally, the effect of Meth-AEA on the proliferation/viability of hPdLCs was measured by MTT method. Results Methanandamide at concentration of 10 µM significantly inhibited P. gingivalis LPS induced production of IL-6, IL-8, and MCP-1. Basal production of IL-6 and IL-8 was slightly enhanced by 10 µM Meth-AEA. No effect of Meth-AEA on the basal production of MCP-1 was observed. Proliferation/viability of hPdLCs was not affected by Meth-AEA in concentrations up to 10 µM and was significantly inhibited by 30 µM Meth-AEA. Conclusion Our study supports the influence of cannabinoid system on the inflammatory processes in periodontal tissue and its potential involvement in the progression of periodontal disease.

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