RTA2 is involved in calcineurin-mediated azole resistance and sphingoid long-chain base release in Candida albicans

2008 ◽  
Vol 66 (1) ◽  
pp. 122-134 ◽  
Author(s):  
X. M. Jia ◽  
Y. Wang ◽  
Y. Jia ◽  
P. H. Gao ◽  
Y. G. Xu ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Azole Resistance ◽  
Chain Base ◽  
Long Chain Base ◽  
Long Chain

scholarly journals Candida albicans sphingolipid C9-methyltransferase is involved in hyphal elongation

Microbiology ◽  
2010 ◽  
Vol 156 (4) ◽  
pp. 1234-1243 ◽  
Author(s):  
Takahiro Oura ◽  
Susumu Kajiwara
Keyword(s):  
Candida Albicans ◽  
Lipid Membrane ◽  
Hyphal Growth ◽  
Pathogenic Fungi ◽  
Wild Type ◽  
Membrane Structures ◽  
Pathogenic Yeast ◽  
Chain Base ◽  
Long Chain Base ◽  
Long Chain

C9-methylated glucosylceramide is a fungus-specific sphingolipid. This lipid is a major membrane component in the cell and is thought to play important roles in the growth and virulence of several fungal species. To investigate the importance of the methyl branch of the long-chain base in glucosylceramides in pathogenic fungi, we identified and characterized a sphingolipid C9-methyltransferase gene (MTS1, C9-MethylTransferase for Sphingolipid 1) in the pathogenic yeast Candida albicans. The mts1 disruptant lacked (E,E)-9-methylsphinga-4,8-dienine in its glucosylceramides and contained (E)-sphing-4-enine and (E,E)-sphinga-4,8-dienine. Reintroducing the MTS1 gene into the mts1 disruptant restored the synthesis of (E,E)-9-methylsphinga-4,8-dienine in the glucosylceramides. We also created a disruptant of the HSX11 gene, encoding glucosylceramide synthase, which catalyses the final step of glucosylceramide synthesis, in C. albicans and compared this mutant with the mts1 disruptant. The C. albicans mts1 and hsx11 disruptants both had a decreased hyphal growth rate compared to the wild-type strain. The hsx11 disruptant showed increased susceptibility to SDS and fluconazole, similar to a previously reported sld1 disruptant that contained only (E)-sphing-4-enine in its glucosylceramides, suggesting that these strains have defects in their cell membrane structures. In contrast, the mts1 disruptant grew similarly to wild-type in medium containing SDS or fluconazole. These results suggest that the C9-methyl group of a long-chain base in glucosylceramides plays an important role in the hyphal elongation of C. albicans independent of lipid membrane disruption.

scholarly journals Long-chain base phosphates modulate pollen tube growth via channel-mediated influx of calcium

2014 ◽  
Vol 79 (3) ◽  
pp. 507-516 ◽  
Author(s):  
Juyou Wu ◽  
Xiaoya Qin ◽  
Shutian Tao ◽  
Xueting Jiang ◽  
Yun-Kuan Liang ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Chain Base ◽  
Long Chain Base ◽  
Long Chain

scholarly journals Intracellular Trafficking Pathway of Yeast Long-chain Base Kinase Lcb4, from Its Synthesis to Its Degradation

2007 ◽  
Vol 282 (39) ◽  
pp. 28485-28492 ◽  
Author(s):  
Soichiro Iwaki ◽  
Takamitsu Sano ◽  
Tomoko Takagi ◽  
Masako Osumi ◽  
Akio Kihara ◽  
...  
Keyword(s):  
Intracellular Trafficking ◽  
Chain Base ◽  
Trafficking Pathway ◽  
Long Chain Base ◽  
Long Chain

scholarly journals Loop 5 region is important for the activity of the long-chain base transporter Rsb1

2016 ◽  
pp. mvw059
Author(s):  
Hiroshi Makuta ◽  
Keisuke Obara ◽  
Akio Kihara
Keyword(s):  
Chain Base ◽  
Long Chain Base ◽  
Long Chain

scholarly journals Accumulation of long-chain bases in yeast promotes their conversion to a long-chain base vinyl ether

2016 ◽  
Vol 57 (11) ◽  
pp. 2040-2050 ◽  
Author(s):  
Fernando Martínez-Montañés ◽  
Museer A. Lone ◽  
Fong-Fu Hsu ◽  
Roger Schneiter
Keyword(s):  
Vinyl Ether ◽  
Chain Base ◽  
Long Chain Base ◽  
Long Chain

Ceramid-1-phosphoethanolamine aus Myxococcus stipitatus/ Ceramide-1-phosphoethanolamines from Myxococcus stipitatus

1988 ◽  
Vol 43 (8) ◽  
pp. 1063-1068 ◽  
Author(s):  
J. Stein ◽  
H. Budzikiewicz
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Component ◽  
Acid Component ◽  
Chain Base ◽  
Long Chain Base ◽  
Ceramide 1 ◽  
Long Chain

AbstractThe structures of six ceramide-1-phosphoethanolamines have been elucidated which differ in the long chain base as well as in the fatty acid component

Characterization of sphingolipid long-chain base kinase in Arabidopsis thaliana

2000 ◽  
Vol 28 (6) ◽  
pp. 747-748 ◽  
Author(s):  
H. Nishiura ◽  
K. Tamura ◽  
Y. Morimoto ◽  
H. Imai
Keyword(s):  
Arabidopsis Thaliana ◽  
Kinase Activity ◽  
Sphingosine Kinase ◽  
Functional Identification ◽  
Chain Base ◽  
Sequence Identity ◽  
Gene Coding ◽  
Long Chain Base ◽  
Long Chain

Sphingolipid long-chain base (LCB) kinase catalyses the phosphorylation of sphingolipid LCB to form LCB 1-phosphate. Based on sequence identity to a murine sphingosine kinase (murine SPHK1a), we isolated and characterized a LCB kinase-like cDNA in Arabidopsis thaliana. The deduced amino acid sequence of the homologous cDNA shows several regions that are highly conserved in LCB kinases from mouse, yeast, human and Caenorhabditis elegans. These regions are not similar to those of other known kinase families. For a functional identification, the homologous cDNA from A. thaliana was expressed in Escherichia coli and LCB kinase activity was measured. The recombinant AtLcbk1 protein was found to utilize ATP and sphinganine. These results indicate the first identification of a gene coding for a LCB kinase in plants.

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