Structural mechanisms of RNA recognition: sequence-specific and non-specific RNA-binding proteins and the Cas9-RNA-DNA complex

2014 ◽  
Vol 72 (6) ◽  
pp. 1045-1058 ◽  
Author(s):  
Ting Ban ◽  
Jian-Kang Zhu ◽  
Karsten Melcher ◽  
H. Eric Xu
Keyword(s):  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Rna Recognition ◽  
Recognition Sequence ◽  
Structural Mechanisms ◽  
Dna Complex

scholarly journals RNA recognition motifs of disease-linked RNA-binding proteins contribute to amyloid formation

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sashank Agrawal ◽  
Pan-Hsien Kuo ◽  
Lee-Ya Chu ◽  
Bagher Golzarroshan ◽  
Monika Jain ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Amyloid Formation ◽  
Rna Recognition ◽  
Rna Recognition Motifs ◽  
Recognition Motifs

scholarly journals RNA recognition motifs involved in nuclear import of RNA-binding proteins

RNA Biology ◽  
2010 ◽  
Vol 7 (3) ◽  
pp. 339-344 ◽  
Author(s):  
Alejandro Cassola ◽  
Griselda Noé ◽  
Alberto C. Frasch
Keyword(s):  
Nuclear Import ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Rna Recognition ◽  
Rna Recognition Motifs ◽  
Recognition Motifs

Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins

2009 ◽  
Vol 27 (7) ◽  
pp. 667-670 ◽  
Author(s):  
Debashish Ray ◽  
Hilal Kazan ◽  
Esther T Chan ◽  
Lourdes Peña Castillo ◽  
Sidharth Chaudhry ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Rna Recognition ◽  
Systematic Analysis

scholarly journals Drosophila Nanos acts as a molecular clamp that modulates the RNA-binding and repression activities of Pumilio

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Chase A Weidmann ◽  
Chen Qiu ◽  
René M Arvola ◽  
Tzu-Fang Lou ◽  
Jordan Killingsworth ◽  
...  
Keyword(s):  
Regulatory Network ◽  
Translational Regulation ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Rna Recognition ◽  
Recognition Sequence ◽  
Repression Complex ◽  
Neuronal Functions ◽  
Rna Complexes

Collaboration among the multitude of RNA-binding proteins (RBPs) is ubiquitous, yet our understanding of these key regulatory complexes has been limited to single RBPs. We investigated combinatorial translational regulation by Drosophila Pumilio (Pum) and Nanos (Nos), which control development, fertility, and neuronal functions. Our results show how the specificity of one RBP (Pum) is modulated by cooperative RNA recognition with a second RBP (Nos) to synergistically repress mRNAs. Crystal structures of Nos-Pum-RNA complexes reveal that Nos embraces Pum and RNA, contributes sequence-specific contacts, and increases Pum RNA-binding affinity. Nos shifts the recognition sequence and promotes repression complex formation on mRNAs that are not stably bound by Pum alone, explaining the preponderance of sub-optimal Pum sites regulated in vivo. Our results illuminate the molecular mechanism of a regulatory switch controlling crucial gene expression programs, and provide a framework for understanding how the partnering of RBPs evokes changes in binding specificity that underlie regulatory network dynamics.

scholarly journals Identification of the RNA recognition element of the RBPMS family of RNA-binding proteins and their transcriptome-wide mRNA targets

RNA ◽  
2014 ◽  
Vol 20 (7) ◽  
pp. 1090-1102 ◽  
Author(s):  
T. A. Farazi ◽  
C. S. Leonhardt ◽  
N. Mukherjee ◽  
A. Mihailovic ◽  
S. Li ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Rna Recognition ◽  
Recognition Element ◽  
Mrna Targets
Sign in / Sign up

Export Citation Format

Share Document