Mutations in hepatocyte nuclear factor 4α (HNF4α) gene associated with diabetes result in greater loss of HNF4α function in pancreatic β-cells than in nonpancreatic β-cells and in reduced activation of the apolipoprotein CIII promoter in hepatic cells

2002 ◽  
Vol 80 (7) ◽  
pp. 423-430 ◽  
Author(s):  
Bénédicte Oxombre ◽  
Ericka Moerman ◽  
Jerôme Eeckhoute ◽  
Pierre Formstecher ◽  
Bernard Laine
Keyword(s):  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Apolipoprotein Ciii ◽  
Hepatic Cells ◽  
Hepatocyte Nuclear Factor 4Α

scholarly journals Hepatocyte Nuclear Factor-4α Is Essential for Glucose-stimulated Insulin Secretion by Pancreatic β-Cells

2005 ◽  
Vol 281 (8) ◽  
pp. 5246-5257 ◽  
Author(s):  
Atsuko Miura ◽  
Kazuya Yamagata ◽  
Masafumi Kakei ◽  
Hiroyasu Hatakeyama ◽  
Noriko Takahashi ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Hepatocyte Nuclear Factor 4Α ◽  
Glucose Stimulated Insulin Secretion

Antagonism between apolipoprotein AI regulatory protein 1, Ear3/COUP-TF, and hepatocyte nuclear factor 4 modulates apolipoprotein CIII gene expression in liver and intestinal cells

1992 ◽  
Vol 12 (4) ◽  
pp. 1708-1718
Author(s):  
M Mietus-Snyder ◽  
F M Sladek ◽  
G S Ginsburg ◽  
C F Kuo ◽  
J A Ladias ◽  
...  
Keyword(s):  
Gene Expression ◽  
Regulatory Protein ◽  
Lipid Binding ◽  
Hepatic Tissue ◽  
Nuclear Factor ◽  
Apolipoprotein Ai ◽  
Hepatocyte Nuclear Factor ◽  
Apolipoprotein Ciii ◽  
Caco2 Cells ◽  
Hepatocyte Nuclear Factor 4

Apolipoprotein CIII (apoCIII), a lipid-binding protein involved in the transport of triglycerides and cholesterol in the plasma, is synthesized primarily in the liver and the intestine. A cis-acting regulatory element, C3P, located at -90 to -66 upstream from the apoCIII gene transcriptional start site (+1), is necessary for maximal expression of the apoCIII gene in human hepatoma (HepG2) and intestinal carcinoma (Caco2) cells. This report shows that three members of the steroid receptor superfamily of transcription factors, hepatocyte nuclear factor 4 (HNF-4), apolipoprotein AI regulatory protein 1 (ARP-1), and Ear3/COUP-TF, act at the C3P site. HNF-4 activates apoCIII gene expression in HepG2 and Caco2 cells, while ARP-1 and Ear3/COUP-TF repress its expression in the same cells. HNF-4 activation is abolished by increasing amounts of ARP-1 or Ear3/COUP-TF, and repression by ARP-1 or Ear3/COUP-TF is alleviated by increasing amounts of HNF-4. HNF-4 and ARP-1 bind with similar affinities to the C3P site, suggesting that their opposing transcriptional effects may be mediated by direct competition for DNA binding. HNF-4 and ARP-1 mRNAs are present within the same cells in the liver and intestine, and protein extracts from hepatic tissue, HepG2, and Caco2 cells contain significantly more HNF-4 than ARP-1 or Ear3/COUP-TF binding activities. These findings suggest that the transcription of the apoCIII gene in vivo is dependent, at least in part, upon the intracellular balance of these positive and negative regulatory factors.

scholarly journals H19 Is Expressed in Hybrid Hepatocyte Nuclear Factor 4α + Periportal Hepatocytes but Not Cytokeratin 19 + Cholangiocytes in Cholestatic Livers

2018 ◽  
Vol 2 (11) ◽  
pp. 1356-1368 ◽  
Author(s):  
YanChao Jiang ◽  
Yi Huang ◽  
ShiYing Cai ◽  
YongFeng Song ◽  
James L. Boyer ◽  
...  
Keyword(s):  
Cytokeratin 19 ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Hepatocyte Nuclear Factor 4Α

Stability of fatty acyl-coenzyme a thioester ligands of hepatocyte nuclear factor-4α and peroxisome proliferator-activated receptor-α

Lipids ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 559-568 ◽  
Author(s):  
Friedhelm Schroeder ◽  
Huan Huang ◽  
Heather A. Hostetler ◽  
Anca D. Petrescu ◽  
Rachel Hertz ◽  
...  
Keyword(s):  
Coenzyme A ◽  
Fatty Acyl ◽  
Peroxisome Proliferator ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Peroxisome Proliferator Activated Receptor ◽  
Hepatocyte Nuclear Factor 4Α ◽  
Acyl Coenzyme A

scholarly journals Hepatocyte nuclear factor 4α regulates megalin expression in proximal tubular cells

2019 ◽  
Vol 17 ◽  
pp. 87-92 ◽  
Author(s):  
Shota Sasaki ◽  
Ayami Hara ◽  
Masakiyo Sakaguchi ◽  
Masaomi Nangaku ◽  
Yusuke Inoue
Keyword(s):  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Proximal Tubular ◽  
Hepatocyte Nuclear Factor 4Α
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