Two critical factors are required for efficient transformation of multiple soybean cultivars: Agrobacterium strain and orientation of immature cotyledonary explant

2003 ◽  
Vol 107 (3) ◽  
pp. 439-447 ◽  
Author(s):  
Tae-Seok Ko ◽  
Sangman Lee ◽  
Sergei Krasnyanski ◽  
Schuyler S. Korban
Keyword(s):  
Critical Factors ◽  
Agrobacterium Strain ◽  
Soybean Cultivars ◽  
Efficient Transformation ◽  
Cotyledonary Explant

High efficiency Agrobacterium-mediated transformation of Pinellia ternata using petiole explants from submerged cultures

Biologia ◽  
2015 ◽  
Vol 70 (10) ◽  
Author(s):  
Yongbo Duan ◽  
Fenglan Zhao ◽  
Qunlong Li ◽  
Shuangqian Shen ◽  
Zhengchun Li ◽  
...  
Keyword(s):  
High Efficiency ◽  
Functional Characterization ◽  
Transformation System ◽  
Submerged Cultures ◽  
Agrobacterium Strain ◽  
Petiole Explants ◽  
Pinellia Ternata ◽  
Efficient Transformation ◽  
Transgenic Lines ◽  
Selection Medium

AbstractThe lack of an efficient transformation system for Pinellia ternata is a major bottleneck in the functional annotation of genes and development of increased stress resistance of this medicinal herb. Here we describe the efficient transformation of P. ternata using petioles from submerged cultures as explants.We used the Agrobacterium strain EHA105, which harbored a pCAMBIA2300-35S-GUS-CaMVterm plasmid containing β-glucuronidase (GUS) as the reporter gene and nptII as the selectable marker. The recovery of transgenic plants was achieved by callus induction on the selection medium followed by shoot induction on the regeneration medium under selection pressure. Pre-culture of explants and ultrasonic treatment during inoculation enhanced the transformation efficiency. Transformation frequency reached 19% based on a GUS assay of independently derived, putative transgenic lines. This transformation system should facilitate the functional characterization of genes of interest and genetic advancements in P. ternata.

Refining our understanding of the “elephant in the room”

2020 ◽  
Vol 43 ◽  
Author(s):  
Andrew Whiten
Keyword(s):  
Social Cognition ◽  
Cultural Evolution ◽  
Cognitive Abilities ◽  
Critical Factors ◽  
Technological Culture

Abstract The authors do the field of cultural evolution a service by exploring the role of non-social cognition in human cumulative technological culture, truly neglected in comparison with socio-cognitive abilities frequently assumed to be the primary drivers. Some specifics of their delineation of the critical factors are problematic, however. I highlight recent chimpanzee–human comparative findings that should help refine such analyses.

EVALUATION OF MECHANICAL SYSTEMS USED IN PERFUSION

1972 ◽  
Vol 68 (2_Supplb) ◽  
pp. S44-S73 ◽  
Author(s):  
Eugene F. Bernstein
Keyword(s):  
Mechanical Systems ◽  
Organ Perfusion ◽  
Critical Factors ◽  
System 2 ◽  
Common Problems ◽  
Mechanical Components ◽  
Perfusion Experiment ◽  
Selection Of

ABSTRACT Among the critical factors in organ perfusion are (1) the mechanical components of the system, (2) the composition of the perfusate, and (3) the perfusing conditions. In this review, particular consideration is given to the pump, the oxygenator, and cannulas in such systems. Emphasis is placed upon the selection of pertinent equipment for the goals of a particular perfusion experiment, based upon the criteria of adequacy of the perfusion. Common problems in organ perfusion are summarized, and potential solutions to the perfusion problem, involving either biologic or mechanical extracorporeal systems, are suggested.

Sign in / Sign up

Export Citation Format

Share Document