pinellia ternata
Recently Published Documents


TOTAL DOCUMENTS

190
(FIVE YEARS 58)

H-INDEX

17
(FIVE YEARS 3)

2022 ◽  
Vol 67 (4) ◽  
pp. 10-17
Author(s):  
Changyang Li ◽  
Hongxiu Lu ◽  
Xianghu Jiang ◽  
Xuefeng Guo ◽  
Hua Zhong ◽  
...  

It has been recognized that Citrus reticulata and Pinellia ternata have a good therapeutic effect on NSCLC. However, the potential mechanism of C. reticulata and P. ternata in the treatment of NSCLC based on network pharmacology analysis is not clear. The “Drug-Component-Target-Disease” network was constructed by Cytoscape, and the protein interaction (PPI) network was constructed by STRING. Our study indicated that 18 active ingredients of C. reticulata and P. Ternata were screened from the TCMSP database, and 56 target genes of C. reticulata and P. Ternata for the treatment of NSCLC were identified, and we constructed the “Drug-Component-Target-Disease” network. In this study, we screened 56 PPI core genes to establish a PPI network. We concluded that the network pharmacology mechanism of the effect of C. reticulata and P. Ternata  on NSCLC may be closely related to the protein expressed by TP53, ESR1, FOS, NCOA3 and MAPK8, and these may play the therapeutic roles by regulating the IL-17 signaling pathway, antigen processing and presentation, microRNAs in cancer and endocrine resistance.


3 Biotech ◽  
2021 ◽  
Vol 11 (11) ◽  
Author(s):  
Hamza Armghan Noushahi ◽  
Zhenxing Zhu ◽  
Aamir Hamid Khan ◽  
Umair Ahmed ◽  
Muhammad Haroon ◽  
...  

Author(s):  
Yuan Zhao ◽  
Xiao–Meng Qin ◽  
Xue–Ping Tian ◽  
Tao Yang ◽  
Rong Deng ◽  
...  

Abstract Background Pinellia ternata (Thunb.) Breit. is a commonly used herb in traditional Chinese medicine, and the main raw material of various Chinese patent medicines. Continuous cropping obstacle (CCO) is the main factor leading to the decline of crop yields and quality. Methods Metagenomics sequencing technology was used to analyze the microbial community and functional genes of continuous cropping (CC) and control (CK) soils of P. ternata. In addition, differences in physicochemical properties, enzyme activities, microbial community composition and the abundance of functional genes in CC and CK were evaluated, as well as the relationship between these factors and CCO. Results Results indicated that CC of P. ternata led to the decline of rhizosphere soil pH, nutrient imbalance and enzyme activity reduction. Metagenomic analysis indicted that CC also changed the composition of the microbial community, causing an increase in the relative abundance of pathogenic microorganisms such as Fusarium, Klebsiella oxytoca and Pectobacterium carotovorum in the P. ternata rhizosphere. The relative abundance of potentially beneficial Burkholderia and Bradyrhizobium was recorded to decrease. Results also showed that there were considerable differences in CC and CK about the abundances of functional genes related to soil enzymes and the degradation of P. ternata allelochemicals, as well as the microbial groups which they belong. These results clarified the effects of CC on the microbial community structure and functional genes of soil. In addition, Burkholderia and Bradyrhizobium might play important roles in enhancing soil fertility and reducing the toxicity of phenolic acids in rhizosphere soil. Conclusions CC of P. ternata changed the physicochemical properties, microbial community and functional genes of rhizosphere soil. Burkholderia and Bradyrhizobium for enhancing soil fertility and reducing the toxicity of phenolic acids might be potentially beneficial. These results provide theoretical guidance for bioremediation of CCO soil of P. ternata and other staple crops. Graphic abstract


2021 ◽  
Vol 5 (4) ◽  
pp. 12-16
Author(s):  
Xu Ding ◽  
Quanhua Song ◽  
Wei Hu

Based on literature reviews and analysis of research reports on Pinellia ternata found locally and abroad in recent years, this article summarizes and arranges them. The research on Pinellia ternata mainly focuses on its cultivation, tissue culture, and so on. There are only a few research on its active components and its regulation mechanism. The wild resources of Pinellia ternata are gradually decreasing, hence it is urgent to take effective measures to protect these wild resources as well as to establish germplasm resources bank and nursery. In order to meet the needs of the domestic market, it is necessary to investigate the distribution of wild Pinellia ternata resources, explore the best growing environment and conditions, artificially cultivate Pinellia ternata, as well as implement resource industrialization, sustainable development, and utilization.


2021 ◽  
Vol 166 ◽  
pp. 113490
Author(s):  
Han Zhang ◽  
Zeyu Zhang ◽  
Yujie Xiong ◽  
Jiang Shi ◽  
Cheng Chen ◽  
...  

Plant Disease ◽  
2021 ◽  
Author(s):  
Fanfan Wang ◽  
ting Mao ◽  
Tao Tang ◽  
Jie Guo ◽  
Yuanyuan Duan ◽  
...  

Pinellia ternata (Thunb.) Makino ex Briet. (banxia, crow-dipper) is a perennial herbaceous plant native to China, Japan, and Korea. A member of the family Araceae, it is considered an invasive weed in parts of Europe and North America. In August 2020, P. ternata plants showing blight symptoms (8% incidence in a 30-ha field) were observed, near Qianjiang City (30°50′N, 112°92′E), Hubei Province, China. Brown water-soaked lesions first appeared on flowers followed by flower blight and leaf and stem rot during periods of more than 80% humidity (Supplementary figure 1). White, cottony mycelia grew from rotted tissues and produced sporangiophores with brown to black sporangiola. To identify the causal agent, 12 diseased samples were surface disinfested with 0.5% sodium hypochlorite and 75% ethyl alcohol, then plated on potato dextrose agar (PDA) maintained at 25°C. Ten fungal isolates were selected by hyphal tip isolation and placed on fresh PDA. White fungal colonies grew rapidly that later turned pale yellow and produced abundant sporangiola in 13 days. Sporangiophores were smooth, hyaline, aseptate, and produced monosporous sporangiola. Sporangiola were ellipsoid, indehiscent, pediculate, brown to dark brown, 8 to 16 × 14 to 21 μm (n = 50) in size, with visible longitudinal striations . Sporangia with a few or many sporangiospores were subglobose, pale brown to brown, and 55 to 165 μm (n = 40) in diameter. Sporangiospores were broadly ellipsoid, brown to pale brown, striate, 8 to 12 × 15 to 25 μm (n=30) in size, with hyaline polar appendages. Based on these morphological characteristics, the fungus was identified as Choanephora cucurbitarum (Berk. & Ravenel) Thaxt. (Kirk 1984). To confirm the identification, the strain QJFY1 was chosen for DNA sequencing. The internal transcribed spacer (ITS) region of rDNA and large subunit (LSU) region of ribosomal RNA were amplified with primers ITS1/ITS4 and NL1/LR3 (Walther et al. 2013) and the amplicons were sequenced. BLAST analysis of the 593bp sequences (accession no. MW295532) and the 699bp sequence(accession no. MW341527)showed ≥99.5% identity with C. cucurbitarum strains CBS 674.93 (GenBank accession no. JN943006.1 and JN939195.1; Supplementary figure 2). Based on morphological and molecular characteristics, the fungus was identified as C. cucurbitarum. Koch’s postulates were fulfilled by inoculating flowers of three healthy 30-day-old P. ternata plants with 50 μL of inoculum suspension (1 x 104 conidia/ml) obtained from 13-day old cultures of C. cucurbitarum isolate QJFY1. Another three plants treated with sterile distilled water served as controls. All plants were placed in a greenhouse with relative humidity of 90% for 2 days and thereafter placed in the glasshouse at 25 ± 1°C. After three days, symptoms similar to those seen under field conditions, were observed on inoculated plants and non-inoculated plants remained healthy. C. cucurbitarum was reisolated and identified by molecular characteristics (ITS and LSU) from inoculated plants. The experiment was repeated thrice with similar results. To our knowledge, this is the first report of Choanephora blight caused by C. cucurbitarum on P. ternata in China and worldwide. Hubei Province is one of the most important banxia producing areas in China and C. cucurbitarum can pose a new threat to banxia production. Our results provide a basis to develop effective measures to manage this disease. References: Kirk, P. M. 1984. Mycol. Pap. 152:1. Walther, G., et al. 2013. Persoonia 30:11. http://dx.doi.org/10.3767/003158513X665070 Acknowledgements Science Funds for Young Scholar of Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences (grant no. 2019ZYCJJ01), Key Research and Development Program of Hubei Province (grant no. 2020BCA059), Support Plan of Hubei Academy of Agricultural Sciences (grant no. 2019fcxjh09), Key Laboratory of Integrated Management of Crops of Central China, Ministry of Agriculture, P. R.China / Hubei Key Laboratory of Crop Disease, Insect Pests and Weeds Control (grant no. 2019ZTSJJ6), Hubei Agricultural Science and Technology Innovation Center Project (grant no.2019-620-003-001)


2021 ◽  
Author(s):  
Yongli SITU ◽  
Li-na LONG ◽  
Hai-jian LI ◽  
Zhi-xin FANG ◽  
Hong NIE

Abstract Background NUDT21 belongs to NUDT families, which is thought to play an essential role in cancer growth and progression in recent years. Abnormal NUDT21 expression is closely related to lung adenocarcinoma (LUAD). However, the expression level, gene regulation network, and prognostic value of NUDT21 in LUAD remain unclear. Besides, the active compounds of Pinellia ternata against LUAD are still not clear yet. Therefore, an in-depth study of the expression and gene regulation network of NUDT21 is of great theoretical significance and clinical demand for discovering new targets and strategies for the treatment of LUAD and the further improvement of the therapeutic effect of LUAD. Also, the targeted NUDT21 active ingredients of Pinellia ternata were sought to provide a theoretical basis for its clinical application in the treatment of LUAD. Methods A variety of online analysis tools were used in this study, including cBioPortal, ONCOMINE, GeneMANIA, GEPIA, Metascape, UALCAN, LinkedOmics, Metascape, TIMER, TRRUST, The Human Protein Atlas, TCMSP, and AutoDock Vina. Results The levels of transcription and expression of NUDT21 were significantly increased in patients with LUAD. Gene altered of NUDT21 was up to 12% in LUAD patients. However, the promoter methylation level of NUDT21 in LUAD was lower compared to normal human. LUAD patients' survival with the low expression level of NUDT21 was better prognostic value than LUAD patients with high expression level. Forty-eight nodes and 572 edges were found in the PPI network constructed with NUDT21 and its neighboring genes. Regulatory region DNA binding, transcription regulatory region DNA binding, and regulatory region nucleic acid binding were the primary function of NUDT21 and its neighboring genes. The KEGG pathway of NUDT21 and its neighboring genes were mainly involved in the apelin signaling pathway, PI3K-Akt signaling pathway, and axon guidance. Our results showed that DNMT1, HDAC1, and MYC were the critical transcription factor targets involved in the network of NUDT21 and its neighboring genes. We also found that CDK1, ATM and PLK1 were main kinase targets in the NUDT21 kinase-target network. The NUDT21 miRNA-target network was associated with MIR-302C, MIR-9, and MIR-330. Moreover, the expression of NUDT21 was positively related to the infiltration of CD8 + T cells, macrophages, neutrophils and dendritic cell. 13 active compounds of Pinellia ternata were retrieved from the TCMSP. Among them, baicalein was the best combination with NUDT21. Conclusions Our results revealed the expression and potential regulatory network of NUDT21 in LUAD, laying a foundation for further research on the role of NUDT21 in cancer. Furthermore, we offer new therapeutic targets and prognostic biomarkers for the reference. Finally, we provide potential therapeutic drugs from traditional Chinese medicine in the treatment of LUAD.


Plant Disease ◽  
2021 ◽  
Author(s):  
Fanfan Wang ◽  
Tao Tang ◽  
ting Mao ◽  
Jie Guo ◽  
XiaoLiang Guo ◽  
...  

Banxia [Pinellia ternata (Thunb.) Breit., Araceae] is a perennial herbaceous plant, where the tuber is commonly used in traditional Chinese herbal medicine. In the summer of 2020, an outbreak of soft rot of Banxia was observed in Zhugentan Town (30°50′N, 112°91′E), Qianjiang City, Hubei Province, with about 56% percentage of infected plants. Symptomatic plants initially appeared with small water-soaked spots on leaves that progressed into extensive translucent spots when facing a light source. The bacteria further spread to the stems and tubers. Infected tubers appeared normal, but inner macerated inclusions exuded when touched. The whole plant was macerated and collapsed within a few days. Ten leaves with typical symptoms were obtained from a diseased field, by surface sterilizing in 75% ethanol for 30 s and 0.3% NaClO for 5 min, washing the tissue sections three times in sterile water. Small pieces of tissue (5 × 5 mm) were removed from lesion borders, plated on nutrient ager medium, and cultivated at 37 ℃ for 48 h. Five representative isolates were selected for further identification. Colonies were all smooth and transparent. In addition, these strains were Gram-negative, and had the ability to reduce D-arabinose, melibiose, galactose, raffinose, rhamnose, inositol, and mannitol, but not reduce 5-keto-D-gluconate, L-xylose, amygdalin, and sorbitol. Genomic DNA was extracted from isolate stain ZG5. The 16S rDNA gene, recombinase A (recA) gene, and DNA polymerase III subunits gamma and tau (dnaX) were amplified by PCR with the primers 27f/1492r (Weisburg et al. 1991), recF/recR (Waleron et al. 2002), and dnaXf/dnaXr (Sławiak et al. 2009), respectively. The PCR products were sequenced, then submitted to GenBank (GenBank MW332472, MW349833, MW349834, respectively). BLAST search showed that the sequences of 16S rDNA, recA, and dnaX respectively matched ≥99% with D. fangzhongdai strains DSM 101947 (CP025003), QZH3 (CP031507), and PA1 (CP020872). Pathogenicity tests were performed on 10 healthy, 3-month-old P. ternate plants. Five plants were injected with 20 μl of bacterial suspension (108 CFU/ml) of isolate ZG5, and other plants were injected with sterile water as a negative control. All tested plants were incubated at 28 ℃ and individually covered with a plastic bag. After 24 h, soft rot symptoms all appeared on the pathogen-inoculated leaves, whereas no symptoms on the control leaves. The pathogenicity test was repeated three times and obtained same results. Koch’s postulates were fulfilled by reisolating D. fangzhongdai from inoculated plants. Meanwhile, PCR were performed on the reisolated bacteria as above described, and the pathogen was identified and confirmed as D. fangzhongdai. Here we report that D. fangzhongdai causes soft rot of P. ternata in China. The disease progressed very rapidly, and reduced the yield and quality of tubers. Thus, more research is needed to implement effective strategies to manage this disease.


Sign in / Sign up

Export Citation Format

Share Document