Detection of two quantitative trait loci for resistance to ascochyta blight in an intra-specific cross of chickpea (Cicer arietinum L.): development of SCAR markers associated with resistance

Hossain, S., Panozzo, J. F., Pittock, C. and Ford, R. 2011. Quantitative trait loci analysis of seed coat color components for selective breeding in chickpea (Cicer arietinumL.). Can. J. Plant Sci. 91: 49–55. Chickpea (Cicer arietinum L.) is an annual grain legume, grown worldwide for human consumption with the potential to attract premium prices in markets such as India, Bangladesh and southern Asia. The ability to accurately select for seed coat color, an important export quality trait, would greatly benefit chickpea breeding programs. In order to determine the major genomic loci governing the color trait, the color components of CIE L* (luminance), CIE a* (red/green color) and CIE b* (blue/yellow color), C* (chroma or saturation of the color) and h° (hue or purity of the color) were mapped, and associated molecular markers were identified. A linkage map was constructed with 80 SSR markers distributed over 10 linkage groups at an average marker density of 2.8 cM. Two major quantitative trait loci (QTL), which accounted for up to 36 and 49% of the genetic variance and several smaller genetic effects were determined to govern the color components. These were consistent across two differing environments. Once validated, the markers that are close to and flanking these QTL and significantly associated with the minor gene effects will be useful in future color selective breeding programs.

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Mapping quantitative trait loci in chickpea associated with time to flowering and resistance to Didymella rabiei the causal agent of Ascochyta blight

Theoretical and Applied Genetics ◽

10.1007/s00122-006-0390-3 ◽

2006 ◽

Vol 113 (7) ◽

pp. 1357-1369 ◽

Cited By ~ 74

Author(s):

Judith Lichtenzveig ◽

David J. Bonfil ◽

Hong-Bin Zhang ◽

Dani Shtienberg ◽

Shahal Abbo

Keyword(s):

Quantitative Trait Loci ◽

Quantitative Trait ◽

Ascochyta Blight ◽

Causal Agent ◽

Trait Loci

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Lens orientalis Contributes Quantitative Trait Loci and Candidate Genes Associated With Ascochyta Blight Resistance in Lentil

Frontiers in Plant Science ◽

10.3389/fpls.2021.703283 ◽

2021 ◽

Vol 12 ◽

Author(s):

Rama Harinath Reddy Dadu ◽

Ido Bar ◽

Rebecca Ford ◽

Prabhakaran Sambasivam ◽

Janine Croser ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Candidate Genes ◽

Quantitative Trait ◽

Mapping Population ◽

Ascochyta Blight ◽

Genotyping By Sequencing ◽

Defense Responses ◽

Phenotypic Variance ◽

Genetic Dissection ◽

Trait Loci

Australian lentil production is affected by several major biotic constraints including Ascochyta blight (AB), caused by Ascochyta lentis, a devastating fungal disease. Cultivation of AB resistant cultivars, alongside agronomic management including fungicide application, is the current most economically viable control strategy. However, the breakdown of AB resistance in cultivars, such as Northfield and Nipper, suggests the need for introgression of new and diverse resistance genes. Successful introgression entails an understanding of the genetic basis of resistance. In this context, a biparental mapping population derived from a cross between a recently identified AB resistant accession ILWL 180 (Lens orientalis) and a susceptible cultivar ILL 6002 was produced. A genetic linkage map was constructed from single-nucleotide polymorphism markers generated using a genotyping-by-sequencing transcript approach. Genetic dissection of the mapping population revealed a major quantitative trait loci (QTL) region nested with three QTLs on linkage group 5 and explained 9.5–11.5 percent (%) of phenotypic variance for AB resistance. Another QTL was identified on LG2 with phenotypic variance of 9.6%. The identified QTL regions harbored putative candidate genes potentially associated with defense responses to A. lentis infection. The QTL analysis and the candidate gene information are expected to contribute to the development of diagnostic markers and enable marker-assisted resistance selection in lentil breeding programmes.

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