Hybrid maize breeding with doubled haploids: V. Selection strategies for testcross performance with variable sizes of crosses and S1 families

2009 ◽  
Vol 120 (4) ◽  
pp. 699-708 ◽  
Author(s):  
Thilo Wegenast ◽  
H. Friedrich Utz ◽  
C. Friedrich H. Longin ◽  
Hans Peter Maurer ◽  
Baldev S. Dhillon ◽  
...  
2010 ◽  
Vol 121 (7) ◽  
pp. 1391-1393 ◽  
Author(s):  
Thilo Wegenast ◽  
H. Friedrich Utz ◽  
C. Friedrich H. Longin ◽  
Hans Peter Maurer ◽  
Baldev S. Dhillon ◽  
...  

2008 ◽  
Vol 117 (2) ◽  
pp. 251-260 ◽  
Author(s):  
Thilo Wegenast ◽  
C. Friedrich H. Longin ◽  
H. Friedrich Utz ◽  
Albrecht E. Melchinger ◽  
Hans Peter Maurer ◽  
...  

2006 ◽  
Vol 112 (5) ◽  
pp. 903-912 ◽  
Author(s):  
C. Friedrich H. Longin ◽  
H. Friedrich Utz ◽  
Jochen C. Reif ◽  
Wolfgang Schipprack ◽  
Albrecht E. Melchinger

2007 ◽  
Vol 115 (4) ◽  
pp. 519-527 ◽  
Author(s):  
C. Friedrich H. Longin ◽  
H. Friedrich Utz ◽  
Jochen C. Reif ◽  
Thilo Wegenast ◽  
Wolfgang Schipprack ◽  
...  

2006 ◽  
Vol 54 (3) ◽  
pp. 343-350 ◽  
Author(s):  
C. F. H. Longin ◽  
H. F. Utz ◽  
A. E. Melchinger ◽  
J.C. Reif

The optimum allocation of breeding resources is crucial for the efficiency of breeding programmes. The objectives were to (i) compare selection gain ΔGk for finite and infinite sample sizes, (ii) compare ΔGk and the probability of identifying superior hybrids (Pk), and (iii) determine the optimum allocation of the number of hybrids and test locations in hybrid maize breeding using doubled haploids. Infinite compared to finite sample sizes led to almost identical optimum allocation of test resources, but to an inflation of ΔGk. This inflation decreased as the budget and the number of finally selected hybrids increased. A reasonable Pk was reached for hybrids belonging to the q = 1% best of the population. The optimum allocations for Pk(q) and ΔGkwere similar, indicating that Pk(q) is promising for optimizing breeding programmes.


Genetics ◽  
2018 ◽  
Vol 210 (3) ◽  
pp. 1125-1138 ◽  
Author(s):  
Joseph L. Gage ◽  
Michael R. White ◽  
Jode W. Edwards ◽  
Shawn Kaeppler ◽  
Natalia de Leon

2006 ◽  
Vol 28 (1) ◽  
pp. 169-175 ◽  
Author(s):  
Kalinka Carla Padovani de Carvalho Salgado ◽  
das Graças Guimarães Carvalho Vieira ◽  
Édila Vilela de Resende Von Pinho ◽  
Cláudia Teixeira Guimarães ◽  
Renzo Garcia Von Pinho ◽  
...  

One of the main features that confer high quality to the seed is its genetic purity, in which one of the major causes of contamination is the self-pollination of the female parent. Up to date, there is no accurate and fast methods for detecting such contamination. Thus, this work was carried out to certify the genetic purity in seeds of hybrid maize using different biochemical and DNA-based markers. Two single-cross hybrids and their parental lines derived from the maize breeding program at UFLA were evaluated by isoenzymatic pattern of alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (ACP), glutamate-oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), 6-phosphoglucomate dehydrogenase (PGDH), catalase (CAT) and ß-glucosidade (ßGLU) and by microsatellites markers. The enzymatic systems that were able to distinguish the hybrids from their parental line were the catalase, the isocitrate dehydrogenase and the esterase. The esterase showed a Mendelian segregation pattern for UFLA 8/3 hybrid, that enables a safer genetic purity certificate. Microsatellites were able to differentiate the hybrid lines and the respective parental lines. Moreover, this technique was fast, precise and without environment effects. For microsatellites, the amplification pattern was identical when young leaves or seeds were used as DNA source. The possibility of using seeds as DNA source would accelerate and facilitate the role process of the genetic purity analysis.


Sign in / Sign up

Export Citation Format

Share Document