Analysis of somatic hybrids between two sterile dihaploid Solanum tuberosum L. breeding lines. Restoration of fertility and complementation of G. pallida Pa2 and Pa3 resistance

1996 ◽  
Vol 92 (3-4) ◽  
pp. 403-410 ◽  
Author(s):  
J. O. Rasmussen ◽  
J. P. Nepper ◽  
O. S. Rasmussen
Author(s):  
Jarosław Przetakiewicz ◽  
Anna Nadolska-Orczyk ◽  
Dominik Kuć ◽  
Wacław Orczyk

AbstractIntraspecific somatic hybrids between 16 different diploid breeding lines of Solanum tuberosum L. were produced by PEG-induced fusion. Manually selected heterokaryons were cultured in a Millicells-CM using a post-fusion protoplast mixture. Plants were regenerated from calli derived from heterokaryons obtained from 10 out of 38 combinations of diploid lines. Of the tested putative somatic hybrids, 14.2% were diploid, 72.8% were tetraploid and 13% pentaploid. The DNA amplification pattern obtained with RAPD or semi-random primers confirmed that 6 fusion combinations were hybrids. In most cases, the morphological traits were intermediate to those of the diploid fusion partners. About 23.0% of the tested somatic hybrids showed variation in their morphology. Of the tested somatic hybrids, 78.0% flowered and 86.0% tuberized. The cytoplasm of 9 diploid lines and 6 somatic hybrid combinations was analysed. Two of the diploid lines had W/S chloroplasts and α or ε mitochondria; the remainder contained T chloroplasts and β mitochondria. All the analysed somatic hybrids carried T chloroplasts and β mitochondria.


1997 ◽  
Vol 47 (3) ◽  
pp. 229-236
Author(s):  
Tetsuya Yamada ◽  
Shuji Misoo ◽  
Takashige Ishii ◽  
Yasuhiro Ito ◽  
Keigo Takaoka ◽  
...  

2008 ◽  
Vol 116 (5) ◽  
pp. 691-700 ◽  
Author(s):  
Ramona Thieme ◽  
Elena Rakosy-Tican ◽  
Tatjana Gavrilenko ◽  
Olga Antonova ◽  
Jörg Schubert ◽  
...  

1997 ◽  
Vol 74 (2) ◽  
pp. 75-86 ◽  
Author(s):  
David S. Douches ◽  
William W. Kirk ◽  
Kazimierz Jastrzebski ◽  
Christopher Long ◽  
Ray Hammerschmidt

Plant Science ◽  
1991 ◽  
Vol 75 (1) ◽  
pp. 107-115 ◽  
Author(s):  
Sylvia Waara ◽  
Anita Wallin ◽  
Tage Eriksson

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