Artesunate inhibits Staphylococcus aureus biofilm formation by reducing alpha-toxin synthesis

2020 ◽  
Author(s):  
Yan Qian ◽  
Li Xia ◽  
Lai Wei ◽  
Di Li ◽  
Weiwei Jiang
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Alpha Toxin

scholarly journals Alpha-Toxin Is Required for Biofilm Formation by Staphylococcus aureus

2003 ◽  
Vol 185 (10) ◽  
pp. 3214-3217 ◽  
Author(s):  
Nicky C. Caiazza ◽  
G. A. O'Toole
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Clinical Settings ◽  
Alpha Toxin ◽  
Flow Conditions ◽  
Alpha Hemolysin ◽  
Integral Role ◽  
Hemolytic Toxin ◽  
Plastic Surfaces

ABSTRACT Staphylococcus aureus is a common pathogen associated with nosocomial infections. It can persist in clinical settings and gain increased resistance to antimicrobial agents through biofilm formation. We have found that alpha-toxin, a secreted, multimeric, hemolytic toxin encoded by the hla gene, plays an integral role in biofilm formation. The hla mutant was unable to fully colonize plastic surfaces under both static and flow conditions. Based on microscopy studies, we propose that alpha-hemolysin is required for cell-to-cell interactions during biofilm formation.

scholarly journals Alpha-Toxin Promotes Staphylococcus aureus Mucosal Biofilm Formation

2012 ◽  
Vol 2 ◽  
Author(s):  
Michele J. Anderson ◽  
Ying-Chi Lin ◽  
Aaron N. Gillman ◽  
Patrick J. Parks ◽  
Patrick M. Schlievert ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Alpha Toxin

scholarly journals Alpha-Toxin Contributes to Biofilm Formation among Staphylococcus aureus Wound Isolates

Toxins ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 157 ◽  
Author(s):  
Michele Anderson ◽  
Emily Schaaf ◽  
Laura Breshears ◽  
Heidi Wallis ◽  
James Johnson ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Alpha Toxin

scholarly journals XerC Contributes to Diverse Forms of Staphylococcus aureus Infection viaagr-Dependent andagr-Independent Pathways

2016 ◽  
Vol 84 (4) ◽  
pp. 1214-1225 ◽  
Author(s):  
Danielle N. Atwood ◽  
Karen E. Beenken ◽  
Allister J. Loughran ◽  
Daniel G. Meeker ◽  
Tamara L. Lantz ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Protein A ◽  
Limited Capacity ◽  
Alpha Toxin ◽  
Extracellular Proteases ◽  
Internal Organs ◽  
Content Type ◽  
Aureus Infection

We demonstrate that mutation ofxerC, which reportedly encodes a homologue of anEscherichia colirecombinase, limits biofilm formation in the methicillin-resistantStaphylococcus aureusstrain LAC and the methicillin-sensitive strain UAMS-1. This was not due to the decreased production of the polysaccharide intracellular adhesin (PIA) in either strain because the amount of PIA was increased in a UAMS-1xerCmutant and undetectable in both LAC and its isogenicxerCmutant. Mutation ofxerCalso resulted in the increased production of extracellular proteases and nucleases in both LAC and UAMS-1, and limiting the production of either class of enzymes increased biofilm formation in the isogenicxerCmutants. More importantly, the limited capacity to form a biofilm was correlated with increased antibiotic susceptibility in both strains in the context of an established biofilmin vivo. Mutation ofxerCalso attenuated virulence in a murine bacteremia model, as assessed on the basis of the bacterial loads in internal organs and overall lethality. It also resulted in the decreased accumulation of alpha toxin and the increased accumulation of protein A. These findings suggest thatxerCmay impact the functional status ofagr. This was confirmed by demonstrating the reduced accumulation of RNAIII and AgrA in LAC and UAMS-1xerCmutants. However, this cannot account for the biofilm-deficient phenotype ofxerCmutants because mutation ofagrdid not limit biofilm formation in either strain. These results demonstrate thatxerCcontributes to biofilm-associated infections and acute bacteremia and that this is likely due toagr-independent and -dependent pathways, respectively.

Biological Evaluation of Selected 1,2,3-triazole Derivatives as Antibacterial and Antibiofilm Agents

2020 ◽  
Vol 20 (24) ◽  
pp. 2186-2191
Author(s):  
Lialyz Soares Pereira André ◽  
Renata Freire Alves Pereira ◽  
Felipe Ramos Pinheiro ◽  
Aislan Cristina Rheder Fagundes Pascoal ◽  
Vitor Francisco Ferreira ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Public Health Problem ◽  
Biological Evaluation ◽  
Major Public Health Problem ◽  
Community Environments ◽  
Scanning Electron

Background: Resistance to antimicrobial agents is a major public health problem, being Staphylococcus aureus prevalent in infections in hospital and community environments and, admittedly, related to biofilm formation in biotic and abiotic surfaces. Biofilms form a complex and structured community of microorganisms surrounded by an extracellular matrix adhering to each other and to a surface that gives them even more protection from and resistance against the action of antimicrobial agents, as well as against host defenses. Methods: Aiming to control and solve these problems, our study sought to evaluate the action of 1,2,3- triazoles against a Staphylococcus aureus isolate in planktonic and in the biofilm form, evaluating the activity of this triazole through Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests. We have also performed cytotoxic evaluation and Scanning Electron Microscopy (SEM) of the biofilms under the treatment of the compound. The 1,2,3-triazole DAN 49 showed bacteriostatic and bactericidal activity (MIC and MBC 128 μg/mL). In addition, its presence interfered with the biofilm formation stage (1/2 MIC, p <0.000001) and demonstrated an effect on young preformed biofilm (2 MICs, p <0.05). Results: Scanning Electron Microscopy images showed a reduction in the cell population and the appearance of deformations on the surface of some bacteria in the biofilm under treatment with the compound. Conclusion: Therefore, it was possible to conclude the promising anti-biofilm potential of 1,2,3-triazole, demonstrating the importance of the synthesis of new compounds with biological activity.

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