Assessment of inhibition kinetics of the growth of strain P5 on pentachlorophenol under steady-state conditions in a nutristat

1996 ◽  
Vol 165 (3) ◽  
pp. 194-200
Author(s):  
M. Rutgers ◽  
Daniel D. Gooch ◽  
Anton M. Breure ◽  
Johan G. Van Andel
Keyword(s):  
Steady State ◽  
Inhibition Kinetics ◽  
Kinetics Of

Assessment of inhibition kinetics of the growth of strain P5 on pentachlorophenol under steady-state conditions in a nutristat

1996 ◽  
Vol 165 (3) ◽  
pp. 194-200 ◽  
Author(s):  
Michiel Rutgers ◽  
Daniel D. Gooch ◽  
Anton M. Breure ◽  
Johan G. Van Andel
Keyword(s):  
Steady State ◽  
Inhibition Kinetics ◽  
Kinetics Of

Inhibition kinetics of phenol degradation from unstable steady-state data

1997 ◽  
Vol 54 (6) ◽  
pp. 567-576 ◽  
Author(s):  
M. Schröder ◽  
C. Müller ◽  
C. Posten ◽  
W.-D. Deckwer ◽  
V. Hecht
Keyword(s):  
Steady State ◽  
Phenol Degradation ◽  
Inhibition Kinetics ◽  
State Data ◽  
Unstable Steady State ◽  
Kinetics Of

Kinetic analysis of mechanism of intestinal Na+-dependent sugar transport

1985 ◽  
Vol 248 (5) ◽  
pp. C498-C509 ◽  
Author(s):  
D. Restrepo ◽  
G. A. Kimmich
Keyword(s):  
Experimental Data ◽  
Steady State ◽  
Sugar Transport ◽  
Enzyme Kinetic ◽  
Steady State Distribution ◽  
State Distribution ◽  
Least Squares Fit ◽  
Coupling Stoichiometry ◽  
Rate Limiting ◽  
Kinetics Of

Zero-trans kinetics of Na+-sugar cotransport were investigated. Sugar influx was measured at various sodium and sugar concentrations in K+-loaded cells treated with rotenone and valinomycin. Sugar influx follows Michaelis-Menten kinetics as a function of sugar concentration but not as a function of Na+ concentration. Nine models with 1:1 or 2:1 sodium:sugar stoichiometry were considered. The flux equations for these models were solved assuming steady-state distribution of carrier forms and that translocation across the membrane is rate limiting. Classical enzyme kinetic methods and a least-squares fit of flux equations to the experimental data were used to assess the fit of the different models. Four models can be discarded on this basis. Of the remaining models, we discard two on the basis of the trans sodium dependence and the coupling stoichiometry [G. A. Kimmich and J. Randles, Am. J. Physiol. 247 (Cell Physiol. 16): C74-C82, 1984]. The remaining models are terter ordered mechanisms with sodium debinding first at the trans side. If transfer across the membrane is rate limiting, the binding order can be determined to be sodium:sugar:sodium.

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